LOCUS       sequence-202108-        8744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd
            generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8744)
  TITLE     David Root CRISPR plasmids
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pXPR_016
FEATURES             Location/Qualifiers
     source          1..8744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        87..1265
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          318..1256
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1213..1233
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1275..2300
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    2319..2907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2372..2392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2790..2801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2979..3212
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3290..3411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3327..3346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3381..3400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3451..3586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3606..3625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3607..3625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3635..3652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3635..3651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3644..3666)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3793..4248
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3880..3899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4090..4111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4274..4378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4379..4447,4448..5239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4597..4616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5410..5998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5899..5918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6152..6169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6286..6307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6322..6327,6328..6345,6346..6352)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6360..6376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6365..6387
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6384..6400
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6384..6400
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6419..6439
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6421..6439
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6467..6693
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6694..6874
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6921..7046
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7539..7772
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7957..8001
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8179..8427
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8179..8199
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8350..8369
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8444..8519
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8575..8692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac gaccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     1321 tcgaaaagtt cgacagcgtg tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     1381 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     1441 gtttctacaa agatcgttat gttcatcggc actttgcatc ggccgcgctc ccgattccgg
     1501 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     1561 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     1621 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     1681 tcggaccgca aggaatcggt caatacacta cgtggcgtga tttcatatgc gcgattgctg
     1741 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     1801 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     1861 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     1921 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     1981 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     2041 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     2101 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     2161 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     2221 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     2281 gtccgagggc aaaggaatag tcgacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     2341 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2401 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2461 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2521 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2581 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2641 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2701 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     2761 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2821 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2881 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     2941 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3001 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     3061 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3121 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3181 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     3241 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3301 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3361 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3421 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3481 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3541 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     3601 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     3661 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3721 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3781 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4021 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4081 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4141 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4201 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     4261 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4321 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4381 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4441 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4501 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4561 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4621 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4681 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4741 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4801 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4861 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4921 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4981 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5041 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5101 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5161 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5221 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5401 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5461 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5521 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5581 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5641 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5701 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5761 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5821 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5881 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5941 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6001 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6061 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6121 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6181 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6241 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6301 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6361 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     6421 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     6481 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     6541 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     6601 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     6661 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     6721 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6781 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6841 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     6901 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     6961 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7021 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7081 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7141 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7201 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7261 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7321 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     7381 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7441 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     7501 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7561 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7621 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7681 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7741 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7801 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7861 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     7921 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     7981 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8041 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8101 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8161 accccgaggg gacccagaga gggcctattt cccatgattc cttcatattt gcatatacga
     8221 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
     8281 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     8341 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     8401 tatatcttgt ggaaaggacg aaacaccgga gacggacgtc tctgttttag agctagaaat
     8461 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     8521 tttttactag tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa
     8581 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     8641 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     8701 ttacagggac agcagagatc cactttggcg ccggctcgag gggg
//
