LOCUS       sequence-202109-        9840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cloning of a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9840)
  TITLE     David Root CRISPR plasmids
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pXPR_045
FEATURES             Location/Qualifiers
     source          1..9840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        89..599
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     518..536
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             614..1102
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    1118..1706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1171..1191)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1589..1600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1709..1725)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1710..1726)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1911..2144
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2216..2337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2253..2272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2307..2326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2377..2512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2532..2551)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2533..2551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2561..2578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2561..2577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2570..2592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2719..3174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2806..2825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3016..3037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3200..3304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3305..3373,3374..4165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3523..3542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4336..4924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4825..4844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5078..5095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5212..5233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5248..5253,5254..5271,5272..5278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5286..5302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5291..5313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5310..5326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5310..5326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5345..5365
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5347..5365
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5393..5619
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5620..5800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5847..5972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6465..6698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6883..6927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7444..7692
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7444..7464
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7615..7634
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9574..9649
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9705..9822
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agcagagatc cactttggcg ccggctcgag ggggcccggg cgggtttatt acagggacag
       61 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
      121 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
      181 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
      241 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
      301 ggttcgcggc gtgccggacg tgacaaacgg aagccgcaag tctcactagt accctcgcag
      361 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
      421 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
      481 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
      541 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
      601 gcgtacggcc accatgaacc cagccatcag cgtcgctctc ctgctctcag tcttgcaggt
      661 gtcccgaggg cagaaggtga ccagcctgac agcctgcctg gtgaaccaaa accttcgcct
      721 ggactgccgc catgagaata acaccaagga taactccatc cagcatgagt tcagcctgac
      781 ccgagagaag aggaagcacg tgctctcagg caccctcggg atacccgagc acacgtaccg
      841 ctcccgcgtc accctctcca accagcccta tatcaaggtc cttaccctag ccaacttcac
      901 caccaaggat gagggcgact acttttgtga gcttcgagtc tcgggcgcga atcccatgag
      961 ctccaataaa agtatcagtg tgtatagaga caaactggtc aagtgtggcg gcataagcct
     1021 gctggttcag aacacatcct ggatgctgct gctgctgctt tccctctccc tcctccaagc
     1081 cctggacttc atttctctgt gaacgcgtta agtcgacaat caacctctgg attacaaaat
     1141 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     1201 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     1261 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     1321 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     1381 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     1441 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     1501 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     1561 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     1621 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     1681 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     1741 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     1801 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     1861 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     1921 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     1981 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2041 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2101 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     2161 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     2221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2341 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     2521 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     2581 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2641 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2701 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2761 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2821 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2881 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2941 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3001 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3061 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3121 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4621 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4921 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4981 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5041 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5101 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5161 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5221 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5281 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5341 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     5401 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     5461 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     5521 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     5581 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     5641 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5701 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5761 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     5821 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     5881 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     5941 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6001 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6061 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6121 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6181 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6241 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6301 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6361 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6421 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6481 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6541 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6601 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6661 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6721 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6781 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6841 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6901 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6961 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7021 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7081 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7141 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag
     7201 cctcgaccac gacgcaatgg agaaagacgg agagcgccaa cggcgtccat ctcgaaggag
     7261 tcgccagcga taaccggagt agttgaaatg gtaataagac gaccaatctg accagcaagg
     7321 aagccaagat gggaaaggtc atgcggcata cgctcggcgc cagtttgaat attagacata
     7381 atttatcctc aagtaagggg gtacacgcgt cagtcgagtc ctgtcgacaa ggtcgggcag
     7441 gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     7501 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     7561 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     7621 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7681 ggacgaaaca ccggagacgg ttgtaaatga gcacacaaaa tacacatgct aaaatattat
     7741 attctatgac ctttataaaa tcaaccaaaa tcttcttttt aataacttta gtatcaataa
     7801 ttagaatttt tatgttcctt tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg
     7861 ctagttttag taactcgcgt tgttttcttc acctttaata atagctactc caccacttgt
     7921 tcctaagcgg tcagctcctg cttcaatcat tttttgagca tcttcaaatg ttctaactcc
     7981 accagctgct ttaactaaag cattgtcttt aacaactgac ttcattagtt taacatcttc
     8041 aaatgttgca cctgattttg aaaatcctgt tgatgtttta acaaattcta atccagcttc
     8101 aacagctatt tcacaagctt tcatgatttc ttcttttgtt aataaacaat tttccataat
     8161 acatttaaca acatgtgatc cagctgcttt ttttacagct ttcatgtctt ctaaaactaa
     8221 ttcataattt ttgtctttta atgcaccaat atttaatacc atatcaattt ctgttgcacc
     8281 atctttaatt gcttcagaaa cttcgaatgc ttttgtagct gttgtgcatg cacctagagg
     8341 aaaacctaca acatttgtta ttcctacatt tgtgcctttt aataattctt tacaatagct
     8401 tgttcaatat gaattaacac aaactgttgc aaaatcaaat tcaattgctt catcacataa
     8461 ttgtttaatt tcagctttcg tagcatcttg ttttaataat gtgtgatcta tatatttgtt
     8521 tagtttcatt ttttctccta tatattcatt tttaatttta attctttaat aatttcgtct
     8581 actttaactt tagcgttttg aacagattca ccaacaccta taaaataaat ttttagttta
     8641 ggttcagttc cacttgggcg aacagcaaat catgacttat cttctaaata aaattttagt
     8701 aagtcttgtc ctggcatatt atacattcca tcgatgtagt cttcaacatt aacaacttta
     8761 agtccagcaa tttgagttaa gggtgttgct ctcaatgatt tcattaatgg ttcaattttt
     8821 aatttctttt cttctggttt aaaattcaag tttaaagtga aagtgtaata tgcacccatt
     8881 tctttaaata aatcttctaa atagtctact aatgttttat tttgtttttt ataaaatcaa
     8941 gcagcctctg ctattaatat agaagcttgt attccatctt tatctctagc tgagtcatca
     9001 attacatatc cataactttc ttcataagca aaaacaaaat ttaatccgtt atcttcttct
     9061 ttagcaattt ctctacccat tcatttaaat ccagttaaag tttttacaat attaactcca
     9121 tatttttcat gagcgattct atcacccaaa tcacttgtta caaaacttga atatagagcc
     9181 ggattttttg gaatgctatt taagcgtttt agatttgata attttcaatc aattaaaatt
     9241 ggtcctgttt gatttccatc taatcttaca aaatgaccat catgttttat tgccattcca
     9301 aatctgtcag catctgggtc attcataata ataatatctg catcatgttt aataccatat
     9361 tcaagcggta tttttcatgc aggatcaaat tctggatttg gatttacaac atttttaaat
     9421 gtttcatctt caaatgcatg ctcttcaacc tcaataacgt tatatcctga ttcacgtaat
     9481 atttttgggg taaatttagt tcctgttcca ttaactgcgc taaaaataat ttttaaatct
     9541 tttttagctt cttgctcttt tttgtacgtc tctgttttag agctagaaat agcaagttaa
     9601 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
     9661 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9721 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9781 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
//
