LOCUS       sequence-202114-        2208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Chinthalapudi K, Mandati V, Zheng J, Sharff AJ, Bricogne G, Griffin 
            PR, Kissil J, Izard T
  TITLE     Lipid binding promotes the open conformation and tumor-suppressive 
            activity of neurofibromin 2.
  JOURNAL   Nat Commun. 2018 Apr 6;9(1):1338. doi: 10.1038/s41467-018-03648-4.
  PUBMED    29626191
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-6"; volume: "9(1):1338. doi"; pages: " 
            10.1038/s41467-018-03648-4"
COMMENT     Sequence Label: pET32a-Trx-PP-hNF2
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             22..348
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             370..387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             397..420
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 catcaccatc accatcaccc catgagcgat aaaattattc acctgactga cgacagtttt
       61 gacacggatg tactcaaagc ggacggggcg atcctcgtcg atttctgggc agagtggtgc
      121 ggtccgtgca aaatgatcgc cccgattctg gatgaaatcg ctgacgaata tcagggcaaa
      181 ctgaccgttg caaaactgaa catcgatcaa aaccctggca ctgcgccgaa atatggcatc
      241 cgtggtatcc cgactctgct gctgttcaaa aacggtgaag tggcggcaac caaagtgggt
      301 gcactgtcta aaggtcagtt gaaagagttc ctcgacgcta acctggccgg ttctggttct
      361 ggccatatgc accatcatca tcatcattct tctggtctgg aagttctgtt ccaggggccc
      421 atggccgggg ccatcgcttc ccgcatgagc ttcagctctc tcaagaggaa gcaacccaag
      481 acgttcaccg tgaggatcgt caccatggac gccgagatgg agttcaattg cgagatgaag
      541 tggaaaggga aggacctctt tgatttggtg tgccggactc tggggctccg agaaacctgg
      601 ttctttggac tgcagtacac aatcaaggac acagtggcct ggctcaaaat ggacaagaag
      661 gtactggatc atgatgtttc aaaggaagaa ccagtcacct ttcacttctt ggccaaattt
      721 tatcctgaga atgctgaaga ggagctggtt caggagatca cacaacattt attcttctta
      781 caggtaaaga agcagatttt agatgaaaag atctactgcc ctcctgaggc ttctgtgctc
      841 ctggcttctt acgccgtcca ggccaagtat ggtgactacg accccagtgt tcacaagcgg
      901 ggatttttgg cccaagagga attgcttcca aaaagggtaa taaatctgta tcagatgact
      961 ccggaaatgt gggaggagag aattactgct tggtacgcag agcaccgagg ccgagccagg
     1021 gatgaagctg aaatggaata tctgaagata gctcaggacc tggagatgta cggtgtgaac
     1081 tactttgcaa tccggaataa aaagggcaca gagctgctgc ttggagtgga tgccctgggg
     1141 cttcacattt atgaccctga gaacagactg acccccaaga tctccttccc gtggaatgaa
     1201 atccgaaaca tctcgtacag tgacaaggag tttactatta aaccactgga taagaaaatt
     1261 gatgtcttca agtttaactc ctcaaagctt cgtgttaata agctgattct ccagctatgt
     1321 atcgggaacc atgatctatt tatgaggaga aggaaagccg attctttgga agttcagcag
     1381 atgaaagccc aggccaggga ggagaaggct agaaagcaga tggagcggca gcgcctcgct
     1441 cgagagaagc agatgaggga ggaggctgaa cgcacgaggg atgagttgga gaggaggctg
     1501 ctgcagatga aagaagaagc aacaatggcc aacgaagcac tgatgcggtc tgaggagaca
     1561 gctgacctgt tggctgaaaa ggcccagatc accgaggagg aggcaaaact tctggcccag
     1621 aaggccgcag aggctgagca ggaaatgcag cgcatcaagg ccacagcgat tcgcacggag
     1681 gaggagaagc gcctgatgga gcagaaggtg ctggaagccg aggtgctggc actgaagatg
     1741 gctgaggagt cagagaggag ggccaaagag gcagatcagc tgaagcagga cctgcaggaa
     1801 gcacgcgagg cggagcgaag agccaagcag aagctcctgg agattgccac caagcccacg
     1861 tacccgccca tgaacccaat tccagcaccg ttgcctcctg acataccaag cttcaacctc
     1921 attggtgaca gcctgtcttt cgacttcaaa gatactgaca tgaagcggct ttccatggag
     1981 atagagaaag aaaaagtgga atacatggaa aagagcaagc atctgcagga gcagctcaat
     2041 gaactcaaga cagaaatcga ggccttgaaa ctgaaagaga gggagacagc tctggatatt
     2101 ctgcacaatg agaactccga caggggtggc agcagcaagc acaataccat taaaaagctc
     2161 accttgcaga gcgccaagtc ccgagtggcc ttctttgaag agctctag
//
