LOCUS       sequence-202117-        4295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Chinthalapudi K, Rangarajan ES, Brown DT, Izard T
  TITLE     Differential lipid binding of vinculin isoforms promotes 
            quasi-equivalent dimerization.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9539-44. doi: 
            10.1073/pnas.1600702113. Epub 2016 Aug 8.
  PUBMED    27503891
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1600702113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-23- 23"; volume: "113"; issue: "34"; pages: "9539-44"
COMMENT     Sequence Label: pcDNA4-GFP-Metavinculin
FEATURES             Location/Qualifiers
     source          1..4295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..30
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             754..777
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4230..4259
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4275..4292
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catcaccacc accatcatca tcaccaccat atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      241 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      481 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaaggga tccctggaag ttctgttcca ggggcccgcc
      781 gccgctatgc cagtgtttca tacgcgcacg atcgagagca tcctggagcc ggtggcacag
      841 cagatctccc acctggtgat aatgcacgag gagggcgagg tggacggcaa agccattcct
      901 gacctcaccg cgcccgtggc cgccgtgcag gcggccgtca gcaacctcgt ccgggttgga
      961 aaagagactg ttcaaaccac tgaggatcag attttgaaga gagatatgcc accagcattt
     1021 attaaggttg agaatgcttg caccaagctt gtccaggcag ctcagatgct tcagtcagac
     1081 ccttactcag tgcctgctcg agattatcta attgatgggt caaggggcat cctctctgga
     1141 acatcagacc tgctccttac cttcgatgag gctgaggtcc gtaaaattat tagagtttgc
     1201 aaaggaattt tggaatatct tacagtggca gaggtggtgg agactatgga agatttggtc
     1261 acttacacaa agaatcttgg gccaggaatg actaagatgg ccaagatgat tgacgagaga
     1321 cagcaggagc tcactcacca ggagcaccga gtgatgttgg tgaactcgat gaacaccgtg
     1381 aaagagttgc tgccagttct catttcagct atgaagattt ttgtaacaac taaaaactca
     1441 aaaaaccaag gcatagagga agctttaaaa aatcgcaatt ttactgtaga aaaaatgagt
     1501 gctgaaatta atgagataat tcgtgtgtta caactcacct cttgggatga agatgcctgg
     1561 gccagcaagg acactgaagc catgaagaga gcattggcct ccatagactc caaactgaac
     1621 caggccaaag gttggctccg tgaccctagt gcctccccag gggatgctgg tgagcaggcc
     1681 atcagacaga tcttagatga agctggaaaa gttggtgaac tctgtgcagg caaagaacgc
     1741 agggagattc tgggaacttg caaaatgcta gggcagatga ctgatcaagt ggctgacctc
     1801 cgtgccagag gacaaggatc ctcaccggtg gccatgcaga aagctcagca ggtatctcag
     1861 ggtctggatg tgctcacagc aaaagtggaa aatgcagctc gcaagctgga agccatgacc
     1921 aactcaaagc agagcattgc aaagaagatc gatgctgctc agaactggct tgcagatcca
     1981 aatggtggac cggaaggaga agagcagatt cgaggtgctt tggctgaagc tcggaaaata
     2041 gcagaattat gtgatgatcc taaagaaaga gatgacattc tacgttccct tggggaaata
     2101 tctgctctga cttctaaatt agcagatcta cgaagacagg ggaaaggaga ttctccagag
     2161 gctcgagcct tggccaaaca ggtggccacg gccctgcaga acctgcagac caaaaccaac
     2221 cgggctgtgg ccaacagcag accggccaaa gcagctgtac accttgaggg caagattgag
     2281 caagcacagc ggtggattga taatcccaca gtggatgacc gtggagtcgg tcaggctgcc
     2341 atccgggggc ttgtggccga agggcatcgt ctggctaatg ttatgatggg gccttatcgg
     2401 caagatcttc tcgccaagtg tgaccgagtg gaccagctga cagcccagct ggctgacctg
     2461 gctgccagag gggaagggga gagtcctcag gcacgagcac ttgcatctca gctccaagac
     2521 tccttaaagg atctaaaagc tcggatgcag gaggccatga ctcaggaagt gtcagatgtt
     2581 ttcagcgata ccacaactcc catcaagctg ttggcagtgg cagccacggc gcctcctgat
     2641 gcgcctaaca gggaagaggt atttgatgag agggcagcta actttgaaaa ccattcagga
     2701 aagcttggtg ctacggccga gaaggcggct gcggttggta ctgctaataa atcaacagtg
     2761 gaaggcattc aggcctcagt gaagacggcc cgagaactca caccccaggt ggtctcggct
     2821 gctcgtatct tacttaggaa ccctggaaat caagctgctt atgaacattt tgagaccatg
     2881 aagaaccagt ggatcgataa tgttgaaaaa atgacagggc tggtggacga agccattgat
     2941 accaaatctc tgttggatgc ttcagaagaa gcaattaaaa aagacctgga caagtgcaag
     3001 gtagctatgg ccaacattca gcctcagatg ctggttgctg gggcaaccag tattgctcgt
     3061 cgggccaacc ggatcctgct ggtggctaag agggaggtgg agaattccga ggatcccaag
     3121 ttccgtgagg ctgtgaaagc tgcctctgat gaattgagca aaaccatctc cccgatggtg
     3181 atggatgcaa aagctgtggc tggaaacatt tccgaccctg gactgcaaaa gagcttcctg
     3241 gactcaggat atcggatcct gggagctgtg gccaaggtca gagaagcctt ccaacctcag
     3301 gagcctgact tcccgccgcc tccaccagac cttgaacaac tccgactaac agatgagctt
     3361 gctcctccca aaccacctct gcctgaaggt gaggtccctc cacctaggcc tccaccacca
     3421 gaggaaaagg atgaagagtt ccctgagcag aaggccgggg aggtgattaa ccagccaatg
     3481 atgatggctg ccagacagct ccatgatgaa gctcgcaaat ggtccagcaa gccgggcatc
     3541 ccagccgctg aggtgggtat aggtgttgta gctgaggcag atgcggccga tgctgctggc
     3601 ttccctgtcc cccctgacat ggaagacgat tacgaacctg agctgctgtt aatgccatcc
     3661 aatcagccgg tcaaccagcc cattctggcc gcggctcagt ccttgcatcg ggaagctacc
     3721 aagtggtcta gtaagggcaa tgacatcatt gcagcagcca agcgcatggc tctgctgatg
     3781 gctgagatgt ctcggctggt aagagggggc agtggtacca agcgggcact cattcagtgt
     3841 gccaaggaca tcgccaaggc ctcagatgag gtgactcggt tggccaagga ggttgccaag
     3901 cagtgcacag ataaacggat tagaaccaac ctcttacagg tatgtgagcg aatcccaacc
     3961 ataagcaccc agctcaaaat cctgtccaca gtgaaggcca ccatgctggg ccggaccaac
     4021 atcagtgatg aggagtctga gcaggccaca gagatgctgg ttcacaatgc ccagaacctc
     4081 atgcagtctg tgaaggagac tgtgcgggaa gctgaagctg cttcaatcaa aattcgaaca
     4141 gatgctggat ttacactgcg ctgggttaga aagactccct ggtaccagtg agaattcgcg
     4201 gccgctcgag tctagagggc ccgcggttcg aacaaaaact catctcagaa gaggatctga
     4261 atatgcatac cggtcatcat caccatcacc attga
//
