LOCUS       sequence-202120-        1224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Chinthalapudi K, Rangarajan ES, Patil DN, George EM, Brown DT, Izard
            T
  TITLE     Lipid binding promotes oligomerization and focal adhesion activity 
            of vinculin.
  JOURNAL   J Cell Biol. 2014 Dec 8;207(5):643-56. doi: 10.1083/jcb.201404128.
  PUBMED    25488920
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-12-8- 8"; volume: "207"; 
            issue: "5"; pages: "643-56"
COMMENT     Sequence Label: pGEX-6P1-Vinculin tail
FEATURES             Location/Qualifiers
     source          1..1224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..684
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ctggaagttc tgttccaggg gcccctggga tccggggagg tgattaacca gccaatgatg
      721 atggctgcca gacagctcca tgatgaagct cgcaaatggt ccagcaaggg caatgacatc
      781 attgcagcag ccaagcgcat ggctctgctg atggctgaga tgtctcggct ggtaagaggg
      841 ggcagtggta ccaagcgggc actcattcag tgtgccaagg acatcgccaa ggcctcagat
      901 gaggtgactc ggttggccaa ggaggttgcc aagcagtgca cagataaacg gattagaacc
      961 aacctcttac aggtatgtga gcgaatccca accataagca cccagctcaa aatcctgtcc
     1021 acagtgaagg ccaccatgct gggccggacc aacatcagtg atgaggagtc tgagcaggcc
     1081 acagagatgc tggttcacaa tgcccagaac ctcatgcagt ctgtgaagga gactgtgcgg
     1141 gaagctgaag ctgcttcaat caaaattcga acagatgctg gatttacact gcgctgggtt
     1201 agaaagactc cctggtacca gtag
//
