LOCUS       sequence-202122-        8085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express STING V55M HAQ for IFN-beta Luciferase reporter assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pMSCV-hygro-STING HAQ+V155M
FEATURES             Location/Qualifiers
     source          1..8085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             39..555
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    619..960
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1027..1443
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1373..1395
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1411..1427
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2618..3117
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2653..2676)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3050..3069
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3135..4160
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3135..4160
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4518..5032
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5194..5210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5218..5224,5225..5242,5243..5248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5263..5284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5401..5418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5572..6160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5652..5671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6331..7122,7123..7191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6954..6973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7192..7296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7364..7382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7420..7442)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7542..7561
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7755..7777
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7905..7924
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
       61 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa
      121 tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat
      181 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      241 gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      301 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      361 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      421 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      481 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
      541 cgggggtctt tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg
      601 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
      661 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
      721 tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg
      781 agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg
      841 tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc
      901 ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg
      961 cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
     1021 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
     1081 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
     1141 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
     1201 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
     1261 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
     1321 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
     1381 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
     1441 gccggaatta gatccatgcc ccactccagc ctgcatccat ccatcccgtg tcccaggggt
     1501 cacggggccc agaaggcagc cttggttctg ctgagtgcct gcctggtgac cctttggggg
     1561 ctaggagagc caccagagca cactctccgg tacctggtgc tccacctagc ctccctgcag
     1621 ctgggactgc tgttaaacgg ggtctgcagc ctggctgagg agctgcacca catccactcc
     1681 aggtaccggg gcagctactg gaggactgtg cgggcctgcc tgggctgccc cctccgccgt
     1741 ggggccctgt tgctgctgtc catctatttc tactactccc tcccaaatgc ggtcggcccg
     1801 cccttcactt ggatgcttgc cctcctgggc ctctcgcagg cactgaacat cctcctgggc
     1861 ctcaagggcc tggccccagc tgagatctct gcagtgtgtg aaaaagggaa tttcaacatg
     1921 gcccatgggc tggcatggtc atattacatc ggatatctgc ggctgatcct gccagagctc
     1981 caggcccgga ttcgaactta caatcagcat tacaacaacc tgctacgggg tgcagtgagc
     2041 cagcggctgt atattctcct cccattggac tgtggggtgc ctgataacct gagtatggct
     2101 gaccccaaca ttcgcttcct ggataaactg ccccagcaga ccgctgaccg tgctggcatc
     2161 aaggatcggg tttacagcaa cagcatctat gagcttctgg agaacgggca gcgggcgggc
     2221 acctgtgtcc tggagtacgc cacccccttg cagactttgt ttgccatgtc acaatacagt
     2281 caagctggct ttagccggga ggataggctt gagcaggcca aactcttctg ccagacactt
     2341 gaggacatcc tggcagatgc ccctgagtct cagaacaact gccgcctcat tgcctaccag
     2401 gaacctgcag atgacagcag cttctcgctg tcccaggagg ttctccggca cctgcggcag
     2461 gaggaaaagg aagaggttac tgtgggcagc ttgaagacct cagcggtgcc cagtacctcc
     2521 acgatgtccc aagagcctga gctcctcatc agtggaatgg aaaagcccct ccctctccgc
     2581 acggatttct cttgactcga ggttaacgaa ttctaccggg taggggaggc gcttttccca
     2641 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     2701 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     2761 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     2821 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     2881 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     2941 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     3001 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     3061 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     3121 tgcatcccgc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     3181 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     3241 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     3301 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     3361 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     3421 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     3481 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     3541 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     3601 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     3661 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     3721 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     3781 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     3841 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     3901 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     3961 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     4021 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     4081 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     4141 gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc
     4201 tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg
     4261 tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt
     4321 cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac
     4381 gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg
     4441 ggtgcagatc cgtcgacctg cagccaagct tatcgataaa ataaaagatt ttatttagtc
     4501 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     4561 cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt
     4621 aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4681 cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta
     4741 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     4801 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     4861 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg
     4921 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     4981 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atgggtaaca
     5041 gtttcttgaa gttggagaac aacattctga gggtaggagt cgaatattaa gtaatcctga
     5101 ctcaattagc cactgttttg aatccacata ctccaatact cctgaaatag ttcattatgg
     5161 acagcgcaga aagagctggg gagaattgtg aaattgttat ccgctcacaa ttccacacaa
     5221 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5281 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5341 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5401 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5581 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5641 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5701 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5761 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5821 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5881 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6001 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7321 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7381 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7441 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7501 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7561 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7621 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7681 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7741 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc
     7801 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     7861 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     7921 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     7981 aggcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact
     8041 caacaatatc accagctgaa gcctatagag tacgagccat agata
//
