LOCUS       sequence-202123-       10448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control lentivector. Coexpression of mTagBFP and gene of interest 
            from CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, 
            Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
  TITLE     Transmission of innate immune signaling by packaging of cGAMP in 
            viral particles.
  JOURNAL   Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
            Epub 2015 Jul 30.
  PUBMED    26229115
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab3628"; journalName: "Science"; date: 
            "2015-09-11- 11"; volume: "349"; issue: "6253"; pages: "1232-6"
COMMENT     Sequence Label: pTRIP-CMV-mTagBFP2-2A
FEATURES             Location/Qualifiers
     source          1..10448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          41..106
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             236..256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    681..815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(718..737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     772..791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             983..1616
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1663..1788
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2281..2514
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2699..2743
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3036..3153
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3252..3555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3556..3759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3710..3730
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3799..4497
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4507..4563
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             4850..5030
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6558..6577)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6791..6810)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6910..6932
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6970..6988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7056..7160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7161..7229,7230..8021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7379..7398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8192..8780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8681..8700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8934..8951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9021..9041)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9026..9355
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9206..9341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
       61 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
      121 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
      181 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
      241 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
      301 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
      361 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
      421 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
      481 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
      541 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
      601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      661 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      781 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
      841 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
      901 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
      961 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     1021 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     1081 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     1141 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     1201 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     1261 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     1321 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     1381 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     1441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1621 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1681 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1741 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1801 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1861 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1921 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1981 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     2041 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     2101 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     2161 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     2221 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     2281 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     2341 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     2401 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     2461 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     2521 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2581 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2641 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2701 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2761 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2821 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2881 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2941 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     3001 tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     3061 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     3121 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     3181 ccactttggc tgatacgcgt atagtaatca attacggggt cattagttca tagcccatat
     3241 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3301 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3421 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3481 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3541 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3601 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     3661 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     3721 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
     3781 ccgcgggcta gcgccaccat gagcgagctg attaaggaga acatgcacat gaagctgtac
     3841 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
     3901 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
     3961 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
     4021 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     4081 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     4141 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
     4201 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
     4261 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
     4321 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
     4381 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
     4441 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatgga
     4501 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     4561 cctggatcca ctagtgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     4621 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     4681 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4741 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4801 aaaatcgtcg agagatgctg catataagca gctgcttttt gcttgtactg ggtctctctg
     4861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     5041 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5101 gccttgacat tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag
     5161 ctgcagaagt acttggaaga agccaccaga gatactcacg attctgcaca tacctggcta
     5221 atcccagatc ctaaggatta cattaagttt actaacattt atataatgat ttatagttta
     5281 aagtataaac ttatctaatt tactattctg acagatatta attaatcctc aaatatcata
     5341 agagatgatt actattatcc ccatttaaca caagaggaaa ctgagaggga aagatgttga
     5401 agtaattttc ccacaattac agcatccgtt agttacgact ctatgatctt ctgacacaaa
     5461 ttccatttac tcctcaccct atgactcagt cgaatatatc aaagttatgg acattatgct
     5521 aagtaacaaa ttaccctttt atatagtaaa tactgagtag attgagagaa gaaattgttt
     5581 gcaaacctga atagcttcaa gaagaagaga agtgaggata agaataacag ttgtcattta
     5641 acaagtttta acaagtaact tggttagaaa gggattcaaa tgcataaagc aagggataaa
     5701 tttttctggc aacaagacta tacaatataa ccttaaatat gacttcaaat aattgttgga
     5761 acttgataaa actaattaaa tattattgaa gattatcaat attataaatg taatttactt
     5821 ttaaaaaggg aacatagaaa tgtgtatcat tagagtagaa aacaatcctt attatcacaa
     5881 tttgtcaaaa caagtttgtt attaacacaa gtagaatact gcattcaatt aagttgactg
     5941 cagattttgt gttttgttaa aattagaaag agataacaac aatttgaatt attgaaagta
     6001 acatgtaaat agttctacat acgttctttt gacatcttgt tcaatcattg atcgaagttc
     6061 tttatcttgg aagaatttgt tccaaagact ctgaaataag gaaaacaatc tattatatag
     6121 tctcacacct ttgttttact tttagtgatt tcaatttaat aatgtaaatg gttaaaattt
     6181 attcttctct gagatcattt cacattgcag atagaaaacc tgagactggg gtaattttta
     6241 ttaaaatcta atttaatctc agaaacacat ctttattcta acatcaattt ttccagtttg
     6301 atattatcat ataaagtcag ccttcctcat ctgcaggttc cacaacaaaa atccaaccaa
     6361 ctgtggatca aaaatattgg gaaaaaatta aaaatagcaa tacaacaata aaaaaataca
     6421 aatcagaaaa acagcacagt ataacaactt tatttagcat ttacaatcta ttaggtatta
     6481 taagtaatct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     6541 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     6601 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     6661 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     6721 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     6781 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     6841 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6901 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6961 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7021 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9001 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9061 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9181 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9301 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9361 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     9421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     9481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     9541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     9601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     9661 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     9721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     9781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     9841 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     9901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     9961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
    10021 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
    10081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
    10141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
    10201 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    10261 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
    10321 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
    10381 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    10441 ggtgtgac
//
