LOCUS       sequence-202125-       12091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express Flag-tagged and shRNA-resistant cGAS and 
            mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, 
            Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
  TITLE     Transmission of innate immune signaling by packaging of cGAMP in 
            viral particles.
  JOURNAL   Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
            Epub 2015 Jul 30.
  PUBMED    26229115
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab3628"; journalName: "Science"; date: 
            "2015-09-11- 11"; volume: "349"; issue: "6253"; pages: "1232-6"
COMMENT     Sequence Label: pTRIP-CMV-mTagBFP2-2A-FLAG-ntcGAS
FEATURES             Location/Qualifiers
     source          1..12091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3254..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3558..3761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3712..3732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3801..4499
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4509..4565
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4581..4604
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6442..6622
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8150..8169)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8383..8402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8502..8524
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8562..8580)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8648..8752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8753..8821,8822..9613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8971..8990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9784..10372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10273..10292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10526..10543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10613..10633)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10618..10947
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10798..10933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttttaagt gtataatgyc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtatagtaat caattacggg gtcattagtt catagcccat
     3241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3781 tcccgcgggc tagcgccacc atgagcgagc tgattaagga gaacatgcac atgaagctgt
     3841 acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc
     3901 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg
     3961 ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc
     4021 agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca
     4081 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     4141 gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc
     4201 agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc
     4261 tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg
     4321 ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact
     4381 atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc
     4441 acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg
     4501 gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg
     4561 gacctggatc cactagtatg gactacaaag acgatgacga caagatgcag ccttggcacg
     4621 gaaaggccat gcagagagct tccgaggccg gagccactgc ccccaaggct tccgcacgga
     4681 atgccagggg cgccccgatg gatcccaacg agtctccggc tgcccccgag gccgccctgc
     4741 ctaaggcggg aaagttcggc cccgccagga agtcgggatc ccggcagaaa aagagcgccc
     4801 cggacaccca ggagaggccg cccgtccgcg caactggggc ccgcgccaaa aaggcccctc
     4861 agcgcgccca ggacacgcag ccgtctgacg ccaccagcgc ccctggggca gaggggctgg
     4921 agcctcctgc ggctcgggag ccggctcttt ccagggctgg ttcttgccgc cagaggggcg
     4981 cgcgctgctc cacgaagcca agacccccgc ccgggccctg ggacgtgccc agccccggcc
     5041 tgccggtctc ggcccccatt ctcgtacgga gggatgcggc gcctggggcc tcgaagctcc
     5101 gggcggtttt ggagaagttg aagctcagcc gcgatgatat ctccacggcg gcggggatgg
     5161 tgaaaggggt tgtggaccac ctgctgctca gactgaagtg cgactccgcg ttcagaggcg
     5221 tcgggctgct gaacaccggg agctactatg agcacgtgaa gatttctgca cctaatgaat
     5281 ttgatgtcat gtttaaactg gaagtcccca gaattcaact agaagaatat tccaacactc
     5341 gtgcatatta ctttgtgaaa tttaaaagaa atccgaaaga aaatcatctg agtcagtttt
     5401 tagaaggtga aatattaagc gcaagcaaaa tgctcagtaa gtttaggaaa atcattaagg
     5461 aagaaattaa cgacattaaa gatacagatg tcatcatgaa gaggaaaaga ggagggagcc
     5521 cagcagttac tttattaata agtgaaaaaa tatctgtgga tataaccctg gctttggaat
     5581 caaaaagtag ctggcctgct agcacccaag aaggcctgcg cattcaaaac tggctttcag
     5641 caaaagttag gaagcagttg aggttgaaac ctttctacct tgtacccaag catgcaaagg
     5701 aaggaaatgg tttccaagaa gaaacatggc ggctatcctt ctctcacatc gaaaaggaaa
     5761 ttttgaacaa tcatggaaaa tctaaaacgt gctgtgaaaa caaagaggaa aagtgctgta
     5821 gaaaggattg tttaaaacta atgaaatacc ttttagaaca gctgaaagaa aggtttaaag
     5881 acaaaaaaca tctggataaa ttctcttctt atcatgtgaa aactgccttc tttcacgtat
     5941 gtacccagaa ccctcaagac agtcagtggg accgcaaaga cctgggcctc tgtttcgaca
     6001 attgtgtcac ttactttctt cagtgcctca ggacagaaaa acttgagaat tattttattc
     6061 ctgaattcaa tctattctct agcaacttaa ttgacaaaag aagtaaagaa tttctgacaa
     6121 agcaaattga atatgaaaga aacaatgagt ttccagtttt tgatgaattt tgactcgaga
     6181 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6241 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6361 actggaaggg ctaattcact cccaacgaag acaaaatcgt cgagagatgc tgcatataag
     6421 cagctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6601 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6661 cttgcaaaga aatgaatatc agagagtgag aggccttgac attataatag atttagcagg
     6721 aattgaacta ggagtggagc acacaggcaa agctgcagaa gtacttggaa gaagccacca
     6781 gagatactca cgattctgca catacctggc taatcccaga tcctaaggat tacattaagt
     6841 ttactaacat ttatataatg atttatagtt taaagtataa acttatctaa tttactattc
     6901 tgacagatat taattaatcc tcaaatatca taagagatga ttactattat ccccatttaa
     6961 cacaagagga aactgagagg gaaagatgtt gaagtaattt tcccacaatt acagcatccg
     7021 ttagttacga ctctatgatc ttctgacaca aattccattt actcctcacc ctatgactca
     7081 gtcgaatata tcaaagttat ggacattatg ctaagtaaca aattaccctt ttatatagta
     7141 aatactgagt agattgagag aagaaattgt ttgcaaacct gaatagcttc aagaagaaga
     7201 gaagtgagga taagaataac agttgtcatt taacaagttt taacaagtaa cttggttaga
     7261 aagggattca aatgcataaa gcaagggata aatttttctg gcaacaagac tatacaatat
     7321 aaccttaaat atgacttcaa ataattgttg gaacttgata aaactaatta aatattattg
     7381 aagattatca atattataaa tgtaatttac ttttaaaaag ggaacataga aatgtgtatc
     7441 attagagtag aaaacaatcc ttattatcac aatttgtcaa aacaagtttg ttattaacac
     7501 aagtagaata ctgcattcaa ttaagttgac tgcagatttt gtgttttgtt aaaattagaa
     7561 agagataaca acaatttgaa ttattgaaag taacatgtaa atagttctac atacgttctt
     7621 ttgacatctt gttcaatcat tgatcgaagt tctttatctt ggaagaattt gttccaaaga
     7681 ctctgaaata aggaaaacaa tctattatat agtctcacac ctttgtttta cttttagtga
     7741 tttcaattta ataatgtaaa tggttaaaat ttattcttct ctgagatcat ttcacattgc
     7801 agatagaaaa cctgagactg gggtaatttt tattaaaatc taatttaatc tcagaaacac
     7861 atctttattc taacatcaat ttttccagtt tgatattatc atataaagtc agccttcctc
     7921 atctgcaggt tccacaacaa aaatccaacc aactgtggat caaaaatatt gggaaaaaat
     7981 taaaaatagc aatacaacaa taaaaaaata caaatcagaa aaacagcaca gtataacaac
     8041 tttatttagc atttacaatc tattaggtat tataagtaat ctagccagat cctctacgcc
     8101 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     8161 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     8221 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     8281 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     8341 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     8401 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8461 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8521 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     8581 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     8641 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8761 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8821 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8881 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8941 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9001 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9061 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9121 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9181 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9241 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9301 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9361 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9421 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9481 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9541 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9601 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9661 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10441 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10501 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10561 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
    10621 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10681 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    10741 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    10801 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    10861 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    10921 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
    10981 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
    11041 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
    11101 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
    11161 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
    11221 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
    11281 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
    11341 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
    11401 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
    11461 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
    11521 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
    11581 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
    11641 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
    11701 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
    11761 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
    11821 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
    11881 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
    11941 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
    12001 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
    12061 cagagattta aagctctaag gtaaatataa a
//
