LOCUS       sequence-202126-       12038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express Flag-tagged and catalytically-inactive cGAS 
            and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, 
            Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
  TITLE     Transmission of innate immune signaling by packaging of cGAMP in 
            viral particles.
  JOURNAL   Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
            Epub 2015 Jul 30.
  PUBMED    26229115
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab3628"; journalName: "Science"; date: 
            "2015-09-11- 11"; volume: "349"; issue: "6253"; pages: "1232-6"
COMMENT     Sequence Label: pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227A
FEATURES             Location/Qualifiers
     source          1..12038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          120..185
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             315..335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    760..894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(797..816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     851..870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1062..1695
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1742..1867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2360..2593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2778..2822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3115..3232
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3331..3634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3635..3838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3789..3809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3878..4576
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4586..4642
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4658..4681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6519..6699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8227..8246)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8460..8479)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8579..8601
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8639..8657)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8725..8829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8830..8898,8899..9690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9048..9067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9861..10449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10350..10369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10603..10620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10690..10710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10695..11024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10875..11010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
       61 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
      121 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
      181 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
      241 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
      301 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
      361 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
      421 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
      481 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
      541 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
      601 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
      661 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      721 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      781 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      841 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
      901 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
      961 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     1021 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     1081 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     1141 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     1201 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     1261 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     1321 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     1381 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     1441 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     1501 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     1561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     1621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     1681 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     1741 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     1801 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     1861 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     1921 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1981 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     2041 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     2101 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     2161 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     2221 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     2281 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     2341 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     2401 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     2461 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     2521 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     2581 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     2641 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     2701 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     2761 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     2821 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     2881 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     2941 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     3001 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     3061 tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa
     3121 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     3181 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     3241 ttacagggac agcagagatc cactttggct gatacgcgta tagtaatcaa ttacggggtc
     3301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     3361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     3841 tttagtgaac cgtcagatcc cgcgggctag cgccaccatg agcgagctga ttaaggagaa
     3901 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     3961 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     4021 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     4081 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     4141 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     4201 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     4261 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     4321 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     4381 ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa
     4441 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     4501 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     4561 ggggcacaag cttaatggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     4621 cgtggaggag aaccctggac ctggatccac tagtatggac tacaaagacg atgacgacaa
     4681 gatgcagcct tggcacggaa aggccatgca gagagcttcc gaggccggag ccactgcccc
     4741 caaggcttcc gcacggaatg ccaggggcgc cccgatggat cccaacgagt ctccggctgc
     4801 ccccgaggcc gccctgccta aggcgggaaa gttcggcccc gccaggaagt cgggatcccg
     4861 gcagaaaaag agcgccccgg acacccagga gaggccgccc gtccgcgcaa ctggggcccg
     4921 cgccaaaaag gcccctcagc gcgcccagga cacgcagccg tctgacgcca ccagcgcccc
     4981 tggggcagag gggctggagc ctcctgcggc tcgggagccg gctctttcca gggctggttc
     5041 ttgccgccag aggggcgcgc gctgctccac gaagccaaga cccccgcccg ggccctggga
     5101 cgtgcccagc cccggcctgc cggtctcggc ccccattctc gtacggaggg atgcggcgcc
     5161 tggggcctcg aagctccggg cggttttgga gaagttgaag ctcagccgcg atgatatctc
     5221 cacggcggcg gggatggtga aaggggttgt ggaccacctg ctgctcagac tgaagtgcga
     5281 ctccgcgttc agaggcgtcg ggctgctgaa caccgggagc tactatgagc acgtgaagat
     5341 ttctgcacct aatgcatttg ctgtcatgtt taaactggaa gtccccagaa ttcaactaga
     5401 agaatattcc aacactcgtg catattactt tgtgaaattt aaaagaaatc cgaaagaaaa
     5461 tcatctgagt cagtttttag aaggtgaaat attatcagct tctaagatgc tgtcaaagtt
     5521 taggaaaatc attaaggaag aaattaacga cattaaagat acagatgtca tcatgaagag
     5581 gaaaagagga gggagccctg ctgtaacact tcttattagt gaaaaaatat ctgtggatat
     5641 aaccctggct ttggaatcaa aaagtagctg gcctgctagc acccaagaag gcctgcgcat
     5701 tcaaaactgg ctttcagcaa aagttaggaa gcaactacga ctaaagccat tttaccttgt
     5761 acccaagcat gcaaaggaag gaaatggttt ccaagaagaa acatggcggc tatccttctc
     5821 tcacatcgaa aaggaaattt tgaacaatca tggaaaatct aaaacgtgct gtgaaaacaa
     5881 agaagagaaa tgttgcagga aagattgttt aaaactaatg aaataccttt tagaacagct
     5941 gaaagaaagg tttaaagaca aaaaacatct ggataaattc tcttcttatc atgtgaaaac
     6001 tgccttcttt cacgtatgta cccagaaccc tcaagacagt cagtgggacc gcaaagacct
     6061 gggcctctgc tttgataact gcgtgacata ctttcttcag tgcctcagga cagaaaaact
     6121 tgagaattat tttattcctg aattcaatct attctctagc aacttaattg acaaaagaag
     6181 taaagaattt ctgacaaagc aaattgaata tgaaagaaac aatgagtttc cagtttttga
     6241 tgaattttga ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     6301 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6361 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6421 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca aaatcgtcga
     6481 gagatgctgc atataagcag ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6721 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     6781 ataatagatt tagcaggaat tgaactagga gtggagcaca caggcaaagc tgcagaagta
     6841 cttggaagaa gccaccagag atactcacga ttctgcacat acctggctaa tcccagatcc
     6901 taaggattac attaagttta ctaacattta tataatgatt tatagtttaa agtataaact
     6961 tatctaattt actattctga cagatattaa ttaatcctca aatatcataa gagatgatta
     7021 ctattatccc catttaacac aagaggaaac tgagagggaa agatgttgaa gtaattttcc
     7081 cacaattaca gcatccgtta gttacgactc tatgatcttc tgacacaaat tccatttact
     7141 cctcacccta tgactcagtc gaatatatca aagttatgga cattatgcta agtaacaaat
     7201 taccctttta tatagtaaat actgagtaga ttgagagaag aaattgtttg caaacctgaa
     7261 tagcttcaag aagaagagaa gtgaggataa gaataacagt tgtcatttaa caagttttaa
     7321 caagtaactt ggttagaaag ggattcaaat gcataaagca agggataaat ttttctggca
     7381 acaagactat acaatataac cttaaatatg acttcaaata attgttggaa cttgataaaa
     7441 ctaattaaat attattgaag attatcaata ttataaatgt aatttacttt taaaaaggga
     7501 acatagaaat gtgtatcatt agagtagaaa acaatcctta ttatcacaat ttgtcaaaac
     7561 aagtttgtta ttaacacaag tagaatactg cattcaatta agttgactgc agattttgtg
     7621 ttttgttaaa attagaaaga gataacaaca atttgaatta ttgaaagtaa catgtaaata
     7681 gttctacata cgttcttttg acatcttgtt caatcattga tcgaagttct ttatcttgga
     7741 agaatttgtt ccaaagactc tgaaataagg aaaacaatct attatatagt ctcacacctt
     7801 tgttttactt ttagtgattt caatttaata atgtaaatgg ttaaaattta ttcttctctg
     7861 agatcatttc acattgcaga tagaaaacct gagactgggg taatttttat taaaatctaa
     7921 tttaatctca gaaacacatc tttattctaa catcaatttt tccagtttga tattatcata
     7981 taaagtcagc cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa
     8041 aaatattggg aaaaaattaa aaatagcaat acaacaataa aaaaatacaa atcagaaaaa
     8101 cagcacagta taacaacttt atttagcatt tacaatctat taggtattat aagtaatcta
     8161 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     8221 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     8281 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     8341 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     8401 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     8461 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8521 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8581 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8641 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8701 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8761 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8821 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8881 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8941 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9001 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9061 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9121 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9181 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9241 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9301 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9361 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9421 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9481 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9541 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9601 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9661 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9721 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9781 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9841 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9901 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9961 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10021 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10081 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10141 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10201 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10261 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10321 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10381 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10441 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10501 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10561 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10621 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10681 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10741 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10801 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10861 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10921 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10981 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
    11041 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
    11101 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
    11161 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
    11221 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
    11281 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
    11341 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    11401 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    11461 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    11521 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    11581 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    11641 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    11701 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    11761 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    11821 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    11881 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    11941 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    12001 gatgccgaac gtaccgacga tgatttatac gatacggt
//
