LOCUS       sequence-202135-        8085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector to expression STING R232.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, 
            Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
  TITLE     Transmission of innate immune signaling by packaging of cGAMP in 
            viral particles.
  JOURNAL   Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
            Epub 2015 Jul 30.
  PUBMED    26229115
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab3628"; journalName: "Science"; date: 
            "2015-09-11- 11"; volume: "349"; issue: "6253"; pages: "1232-6"
COMMENT     Sequence Label: pMSCV-hygro-STING R232
FEATURES             Location/Qualifiers
     source          1..8085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             94..610
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    674..1015
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1082..1498
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1428..1450
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1466..1482
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2673..3172
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2708..2731)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3105..3124
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3190..4215
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3190..4215
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4573..5087
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5249..5265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5273..5279,5280..5297,5298..5303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5318..5339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5456..5473)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5627..6215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5707..5726)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6386..7177,7178..7246))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7009..7028
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7247..7351)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7419..7437
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7475..7497)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7597..7616
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7810..7832
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7960..7979
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
       61 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
      121 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
      181 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
      241 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
      301 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
      361 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
      421 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
      481 tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa
      541 tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg
      601 gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc
      661 cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt
      721 ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat
      781 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
      841 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
      901 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
      961 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
     1021 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
     1081 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
     1141 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
     1201 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
     1261 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
     1321 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
     1381 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
     1441 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
     1501 aattagatcc atgccccact ccagcctgca tccatccatc ccgtgtccca ggggtcacgg
     1561 ggcccagaag gcagccttgg ttctgctgag tgcctgcctg gtgacccttt gggggctagg
     1621 agagccacca gagcacactc tccggtacct ggtgctccac ctagcctccc tgcagctggg
     1681 actgctgtta aacggggtct gcagcctggc tgaggagctg cgccacatcc actccaggta
     1741 ccggggcagc tactggagga ctgtgcgggc ctgcctgggc tgccccctcc gccgtggggc
     1801 cctgttgctg ctgtccatct atttctacta ctccctccca aatgcggtcg gcccgccctt
     1861 cacttggatg cttgccctcc tgggcctctc gcaggcactg aacatcctcc tgggcctcaa
     1921 gggcctggcc ccagctgaga tctctgcagt gtgtgaaaaa gggaatttca acgtggccca
     1981 tgggctggca tggtcatatt acatcggata tctgcggctg atcctgccag agctccaggc
     2041 ccggattcga acttacaatc agcattacaa caacctgcta cggggtgcag tgagccagcg
     2101 gctgtatatt ctcctcccat tggactgtgg ggtgcctgat aacctgagta tggctgaccc
     2161 caacattcgc ttcctggata aactgcccca gcagaccggt gaccgtgctg gcatcaagga
     2221 tcgggtttac agcaacagca tctatgagct tctggagaac gggcagcggg cgggcacctg
     2281 tgtcctggag tacgccaccc ccttgcagac tttgtttgcc atgtcacaat acagtcaagc
     2341 tggctttagc cgggaggata ggcttgagca ggccaaactc ttctgccgga cacttgagga
     2401 catcctggca gatgcccctg agtctcagaa caactgccgc ctcattgcct accaggaacc
     2461 tgcagatgac agcagcttct cgctgtccca ggaggttctc cggcacctgc ggcaggagga
     2521 aaaggaagag gttactgtgg gcagcttgaa gacctcagcg gtgcccagta cctccacgat
     2581 gtcccaagag cctgagctcc tcatcagtgg aatggaaaag cccctccctc tccgcacgga
     2641 tttctcttga ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca
     2701 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2761 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2821 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2881 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2941 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     3001 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     3061 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     3121 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat
     3181 cccgccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     3241 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     3301 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     3361 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3421 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3481 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     3541 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     3601 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     3661 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     3721 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     3781 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     3841 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     3901 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     3961 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     4021 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     4081 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     4141 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     4201 agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc
     4261 cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca
     4321 cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag
     4381 tgattcaggc ccttctggat tgtgttggtc cccagggcac gattgtcatg cccacgcact
     4441 cgggtgatct gactgatccc gcagattgga gatcgccgcc cgtgcctgcc gattgggtgc
     4501 agatccgtcg acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag
     4561 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     4621 ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa
     4681 cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     4741 ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa
     4801 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     4861 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     4921 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc
     4981 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     5041 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc
     5101 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa
     5161 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc
     5221 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac
     5281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6061 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6181 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6241 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6301 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6361 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6421 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6481 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6541 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6601 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6661 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6721 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6781 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6841 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6901 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6961 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7021 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7081 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7141 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7201 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7261 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7321 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7381 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7441 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7501 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7561 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7621 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7681 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7741 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7801 cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga
     7861 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     7921 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     7981 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggcg
     8041 attagtccaa tttgttaaag acaggatatc agtggtccag gctct
//
