LOCUS       sequence-202157-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in 
            Escherichia coli as a GST fusion protein that can be cut with 
            PreScission(TM) protease to yield active enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ribeiro JM, Canales J, Cabezas A, Rodrigues JR, Pinto RM, 
            Lopez-Villamizar I, Costas MJ, Cameselle JC
  TITLE     Specific cyclic ADP-ribose phosphohydrolase obtained by mutagenic 
            engineering of Mn(2+)-dependent ADP-ribose/CDP-alcohol 
            diphosphatase.
  JOURNAL   Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.1038/s41598-017-18393-9.
  PUBMED    29348648
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-01-18"; volume: "8(1):1036. doi"; pages: " 
            10.1038/s41598-017-18393-9"
COMMENT     Sequence Label: pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggcccctgg gatccccgaa ttcccgggtc gacatggatg ataaacccaa ccctgaagcc
       61 ctaagtgaca gttcagagcg tcttttctcc tttggcgtca tcgcagatgt tcaatttgca
      121 gacttagaag atggctttaa tgcacaagga accaggcggc gatactacag acatagtctt
      181 cttcacttac agggtgccat tgaagactgg aataatgaaa gcagcatgcc ctgttgtgtc
      241 cttcagcttg gagatatcat cgatggatat aatgcacagt ataatgcatc caaaaagtcc
      301 ctagaacttg ttatggacat gttcaagagg cttaaagttc cagttcatca tacatgggga
      361 aaccatgaat tctataactt cagtagagag tatttaacac actctaaact taacactaag
      421 tttctagaag atcagattgt acatcatcct gagaccatgc cttcagaaga ttattatgct
      481 tatcattttg taccattccc taaattccgg ttcattttac ttgatgcata tgacttgagt
      541 gtcttgggcg tggatcagtc ttctccaaaa tacgagcagt gtatgaagat attgagggag
      601 cacaatccaa atacggaatt caatagccct caaggacttt ctgagcccca gtttgtccag
      661 tttaatggag gattcagcca agaacagcta aactggttga atgaagtgct aacattctct
      721 gacacaaacc aagaaaaggt ggtgattgtg agccatcttc ccatttaccc ggacgcgtct
      781 gacaatgctg gcctggcctg gaactacaga gatgccctgg cagtcatttg gtctcatgag
      841 tgtgtggtgt gtttctttgc tggtcacacc catgatggtg gctactctga ggatcctttt
      901 ggtgtatacc acgtcaacct agaaggagtt attgaaacag ctccagacag ccaagccttt
      961 ggcacagttc atgtctatcc tgacaaaatg atgttgaaag ggagaggcag agttccagat
     1021 agaattatga attacaagaa agaaagagca ttccattgtt ag
//
