LOCUS       sequence-202167-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1510
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             688..744
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             745..4848
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             745..4848
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4849..4896
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4908..5156
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        5236..5340
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6408..6996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6897..6916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7150..7167
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg
       61 tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt tacttcctgc aggaagcatg
      121 gaccactacc tcgacatcag gctcaggcca gacccagagt tcccaccagc ccagctcatg
      181 tccgtcctct tcggcaagct ccaccaggcc ctcgtggccc agggcggcga caggatcggc
      241 gtgtccttcc cagacctcga cgagtccagg tccaggctcg gcgagaggct ccgcatccac
      301 gcctccgccg acgacctcag ggccctcctc gccaggccgt ggctggaggg cctcagggac
      361 cacctccagt tcggcgagcc agccgtggtg ccacacccaa ccccatacag gcaagtgtcc
      421 agggtgcaag ccaagtccaa cccagagagg ctcaggagga ggctcatgag gaggcacgac
      481 ctctccgagg aagaggccag gaagcgcatc ccagacaccg tggccagggc cctcgacctc
      541 ccattcgtga ccctcaggtc ccagtccacc ggccagcact tccgcctctt catcaggcac
      601 ggcccactcc aggtgaccgc cgaggagggc ggctttacct gctacggcct ctccaagggc
      661 ggcttcgtgc cgtggttcgg ctccggcgcc accaacttct ccctcctcaa gcaagccggc
      721 gacgtggagg agaacccagg cccaatggac aagaagtact cgatcggcct cgacatcggg
      781 acgaactcag ttggctgggc cgtgatcacc gacgagtaca aggtgccctc taagaagttc
      841 aaggtcctgg ggaacaccga ccgccattcc atcaagaaga acctcatcgg cgctctcctg
      901 ttcgacagcg gggagaccgc tgaggctacg aggctcaaga gaaccgctag gcgccggtac
      961 acgagaagga agaacaggat ctgctacctc caagagattt tctccaacga gatggccaag
     1021 gttgacgatt cattcttcca ccgcctggag gagtctttcc tcgtggagga ggataagaag
     1081 cacgagcggc atcccatctt cggcaacatc gtggacgagg ttgcctacca cgagaagtac
     1141 cctacgatct accatctgcg gaagaagctc gtggactcca ccgataaggc ggacctcaga
     1201 ctgatctacc tcgctctggc ccacatgatc aagttccgcg gccatttcct gatcgagggg
     1261 gatctcaacc cagacaacag cgatgttgac aagctgttca tccaactcgt gcagacctac
     1321 aaccaactct tcgaggagaa cccgatcaac gcctctggcg tggacgcgaa ggctatcctg
     1381 tccgcgaggc tctcgaagtc caggaggctg gagaacctga tcgctcagct cccaggcgag
     1441 aagaagaacg gcctgttcgg gaacctcatc gctctcagcc tggggctcac cccgaacttc
     1501 aagtcgaact tcgatctcgc tgaggacgcc aagctgcaac tctccaagga cacctacgac
     1561 gatgacctcg ataacctcct ggcccagatc ggcgatcaat acgcggacct gttcctcgct
     1621 gccaagaacc tgtcggacgc catcctcctg tcagatatcc tccgcgtgaa caccgagatc
     1681 acgaaggctc cactctctgc ctccatgatc aagcgctacg acgagcacca tcaggatctg
     1741 accctcctga aggcgctggt ccgccaacag ctcccggaga agtacaagga gattttcttc
     1801 gatcagtcga agaacggcta cgctgggtac atcgacggcg gggcctcaca agaggagttc
     1861 tacaagttca tcaagccaat cctggagaag atggacggca cggaggagct cctggtgaag
     1921 ctcaacaggg aggacctcct gcggaagcag agaaccttcg ataacggcag catcccccac
     1981 caaatccatc tcggggagct gcacgccatc ctgagaaggc aagaggactt ctaccctttc
     2041 ctcaaggata accgggagaa gatcgagaag atcctgacct tcagaatccc atactacgtc
     2101 ggccctctcg cgcgggggaa ctcaagattc gcttggatga cccgcaagtc tgaggagacc
     2161 atcacgccgt ggaacttcga ggaggtggtg gacaagggcg ctagcgctca gtcgttcatc
     2221 gagaggatga ccaacttcga caagaacctg cccaacgaga aggtgctccc taagcactcg
     2281 ctcctgtacg agtacttcac cgtctacaac gagctcacga aggtgaagta cgtcaccgag
     2341 ggcatgcgca agccagcgtt cctgtccggg gagcagaaga aggctatcgt ggacctcctg
     2401 ttcaagacca accggaaggt cacggttaag caactcaagg aggactactt caagaagatc
     2461 gagtgcttcg attcggtcga gatcagcggc gttgaggacc gcttcaacgc cagcctcggg
     2521 acctaccacg atctcctgaa gatcatcaag gataaggact tcctggacaa cgaggagaac
     2581 gaggatatcc tggaggacat cgtgctgacc ctcacgctgt tcgaggacag ggagatgatc
     2641 gaggagcgcc tgaagacgta cgcccatctc ttcgatgaca aggtcatgaa gcaactcaag
     2701 cgccggagat acaccggctg ggggaggctg tcccgcaagc tcatcaacgg catccgggac
     2761 aagcagtccg ggaagaccat cctcgacttc ctcaagagcg atggcttcgc caacaggaac
     2821 ttcatgcaac tgatccacga tgacagcctc accttcaagg aggatatcca aaaggctcaa
     2881 gtgagcggcc agggggactc gctgcacgag catatcgcga acctcgctgg ctcccccgcg
     2941 atcaagaagg gcatcctcca gaccgtgaag gttgtggacg agctcgtgaa ggtcatgggc
     3001 cggcacaagc ctgagaacat cgtcatcgag atggccagag agaaccaaac cacgcagaag
     3061 gggcaaaaga actctaggga gcgcatgaag cgcatcgagg agggcatcaa ggagctgggg
     3121 tcccaaatcc tcaaggagca cccagtggag aacacccaac tgcagaacga gaagctctac
     3181 ctgtactacc tccagaacgg cagggatatg tacgtggacc aagagctgga tatcaaccgc
     3241 ctcagcgatt acgacgtcga tcatatcgtt ccccagtctt tcctgaagga tgactccatc
     3301 gacaacaagg tcctcaccag gtcggacaag aaccgcggca agtcagataa cgttccatct
     3361 gaggaggtcg ttaagaagat gaagaactac tggaggcagc tcctgaacgc caagctgatc
     3421 acgcaaagga agttcgacaa cctcaccaag gctgagagag gcgggctctc agagctggac
     3481 aaggccggct tcatcaagcg gcagctggtc gagaccagac aaatcacgaa gcacgttgcg
     3541 caaatcctcg actctcggat gaacacgaag tacgatgaga acgacaagct gatcagggag
     3601 gttaaggtga tcaccctgaa gtctaagctc gtctccgact tcaggaagga tttccagttc
     3661 tacaaggttc gcgagatcaa caactaccac catgcccatg acgcttacct caacgctgtg
     3721 gtcggcaccg ctctgatcaa gaagtaccca aagctggagt ccgagttcgt gtacggggac
     3781 tacaaggttt acgatgtgcg caagatgatc gccaagtcgg agcaagagat cggcaaggct
     3841 accgccaagt acttcttcta ctcaaacatc atgaacttct tcaagaccga gatcacgctg
     3901 gccaacggcg agatccggaa gagaccgctc atcgagacca acggcgagac gggggagatc
     3961 gtgtgggaca agggcaggga tttcgcgacc gtccgcaagg ttctctccat gccccaggtg
     4021 aacatcgtca agaagaccga ggtccaaacg ggcgggttct caaaggagtc tatcctgcct
     4081 aagcggaaca gcgacaagct catcgccaga aagaaggact gggacccaaa gaagtacggc
     4141 gggttcgaca gccctaccgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc
     4201 aagtccaaga agctcaagag cgtgaaggag ctcctgggga tcaccatcat ggagaggtcc
     4261 agcttcgaga agaacccaat cgacttcctg gaggccaagg gctacaagga ggtgaagaag
     4321 gacctgatca tcaagctccc gaagtactct ctcttcgagc tggagaacgg caggaagaga
     4381 atgctggctt ccgctggcga gctccagaag gggaacgagc tcgcgctgcc aagcaagtac
     4441 gtgaacttcc tctacctggc ttcccactac gagaagctca agggcagccc ggaggacaac
     4501 gagcaaaagc agctgttcgt cgagcagcac aagcattacc tcgacgagat catcgagcaa
     4561 atctccgagt tcagcaagcg cgtgatcctc gccgacgcga acctggataa ggtcctctcc
     4621 gcctacaaca agcaccggga caagcccatc agagagcaag cggagaacat catccatctc
     4681 ttcaccctga cgaacctcgg cgctcctgct gctttcaagt acttcgacac cacgatcgat
     4741 cggaagagat acacctccac gaaggaggtc ctggacgcga ccctcatcca ccagtcgatc
     4801 accggcctgt acgagacgag gatcgacctc tcacaactcg gcggggataa gagacccgca
     4861 gcaaccaaga aggcagggca agcaaagaag aagaagtgac tcgagtatat gaagatgaag
     4921 atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg
     4981 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta
     5041 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca
     5101 gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc
     5161 tcgttaaccg gtgacggacg tgggcaggtg tgatgaaaag ccttagggtc aggtggcact
     5221 tttcggggaa atgtgcgatg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5281 tatccgctaa tgagacaata accctgataa atgcttcaat tatattgaaa aaggaagagt
     5341 atgagtatcc aacatttccg tgtcgccctt attccctttt ttgcggcctt ctgccttcct
     5401 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc
     5461 cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc
     5521 gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc
     5581 cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5641 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5701 tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
     5761 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5821 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5881 cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct
     5941 tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc
     6001 tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6061 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6121 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6181 tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctgatg
     6241 tgcgggcttt tttttggtgg tggttaccag tgtcgcttag ctaccgtagg tcctcttagg
     6301 cggtccgatt agcgacatag tcatcaatgc cttgacggca aaatccctta acgtgagttt
     6361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6481 ttgccggatc aagagctact aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6541 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6721 ggctgaacgg ggggttcgtg ctcacagccc agcttggagc gaacgaccta caccgaactg
     6781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7021 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7081 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7141 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccacctgc tcatgatctg
     7201 caggcgcgcc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt
     7261 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
     7321 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
     7381 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
     7441 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
     7501 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
     7561 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
     7621 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
     7681 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
     7741 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
     7801 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
     7861 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
     7921 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
     7981 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
     8041 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
     8101 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatctccc
     8161 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct
     8221 ctctaccttc tctagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     8281 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg
     8341 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     8401 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     8461 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     8521 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     8581 gtcgttctag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     8641 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     8701 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     8761 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct
     8821 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     8881 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     8941 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     9001 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     9061 tattttgatc ttgatatact tggatgatgg cat
//
