LOCUS       sequence-202168-        5250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I 
            ccdb cassette for repeat cloning, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3306
FEATURES             Location/Qualifiers
     source          1..5250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..66
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(841..1146)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(943..962)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             2130..2279
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2741..2845
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3913..4501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4402..4421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4655..4672
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gattataagg atcacgatgg cgactacaag gaccacgata ttgactacaa agacgatgac
       61 gataaaatgg ctcctaagaa aaagcgcaaa gtcggtatcc atggcgttcc cgctagcgtg
      121 tcccggaccc ggctgccatc tccccctgcg ccctctcctg cgttctcggc gggcagcttc
      181 agcgatctgc tccgtccgtt cgatccgtcg cttcttgata catcgcttct tgattcgatg
      241 cctgccgtcg gcacgccgca tacagcggct gccccagcag agtgggatga ggcgcaatcg
      301 gctctgcgtg cagccgatga cccgccaccc accgtgcgtg tcgctgtcac tgccgcgcgg
      361 ccgccgcgcg ccaagccggc cccgcgacgg cgtgctgcgc aaccctccga cgcttcgccg
      421 gccgcgcagg ttgatctacg cacgctcggc tacagtcagc agcagcaaga gaagatcaaa
      481 ccgaaggtgc gttcgacagt ggcgcagcac cacgaggcac tggtgggcca tgggtttaca
      541 cacgcgcaca tcgttgcgct cagccaacac ccggcagcgt tagggaccgt cgctgtcacg
      601 tatcagcaca taatcacggc gttgccagag gcgacacacg aagacatcgt tggcgtcggc
      661 aaacagtggt ccggcgcacg cgccctggag gccttgctca cggatgcggg ggagttgaga
      721 ggtccgccgt tacagttgga cacaggccaa cttgtgaaga ttgcaaaacg tggcggcgtg
      781 accgcaatgg aggcagtgca tgccagcagg aatgcgttaa caggtgcccc cctggagacg
      841 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
      901 atcagccact tcttccccga taacggaaac cggcacactg gccatatcgg tggtcatcat
      961 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     1021 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     1081 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     1141 ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
     1201 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
     1261 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
     1321 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctcct
     1381 tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc
     1441 aaatacgcat actgttatct ggcttcgtct ccaacgacca cctcgtcgcc ttggcctgcc
     1501 tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg ccggaattga
     1561 tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcc gactacgcgc
     1621 aagtggttcg cgtgctggag tttttccagt gccactccca cccagcgtac gcatttgatg
     1681 aggccatgac gcagttcggg atgagcagga acgggttggt acagctcttt cgcagagtgg
     1741 gcgtcaccga actcgaagcc cgcggtggaa cgctcccccc agcctcgcag cgttgggacc
     1801 gtatcctcca ggcatcaggg atgaaaaggg ccaaaccgtc ccctacttca gctcaaacac
     1861 cggatcaggc gtctttgcat gcattcgccg attcgctgga gcgtgacctt gatgcgccca
     1921 gcccaatgca cgagggagat cagacgcggg caagcagccg taaacggtcc cgatcggatc
     1981 gtgctgtcac cggcccctcc gcacagcagg ctgtcgaggt gcgcgttccc gaacagcgcg
     2041 atgcgctgca tttgcccctc agctggaggg taaaacgccc gcgtaccagg atctggggcg
     2101 gcctcccgga tccgggttca ggacgggctg acgcattgga cgattttgat ctggatatgc
     2161 tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat gcccttgatg
     2221 actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg gacatgctga
     2281 ttaactctag atgactcgag gttcgagtat tatggcattg ggaaaactgt ttttcttgta
     2341 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga
     2401 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt
     2461 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg
     2521 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat
     2581 atgaggagta aaacacttgt agttgtgagc tcgcgcgtag tcctcggtaa tatcgcagaa
     2641 caaaagtacc tgatatcgag tgtacttcaa gtcacaccgg cgagtgacta gcaggtgatg
     2701 aaaagcctta gggtcaggtg gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt
     2761 atttttctaa atacattcaa atatgtatcc gctaatgaga caataaccct gataaatgct
     2821 tcaattatat tgaaaaagga agagtatgag tatccaacat ttccgtgtcg cccttattcc
     2881 cttttttgcg gccttctgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2941 agatgctgaa gatcagttgg gtgcccgagt gggttacatt gaactggatc tcaacagcgg
     3001 caaaatctta gagagttttc gccccgaaga acgcttccca atgatgagca ctttcaaagt
     3061 tctgctatgt ggcgcggtgt tatcccgtat tgatgctggg caagagcaac tcggtcgccg
     3121 catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac
     3181 ggatggcatg acagtaagag aattatgtag tgctgccata accatgagtg ataacactgc
     3241 ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa
     3301 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3361 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaactctcc gcaaactatt
     3421 aactggcgaa ctacttactc ttgcttcacg ccagcaactc attgactgga tggaggcgga
     3481 caaagttgca ggaccacttc tgcgctcggc acttccggct ggctggttta ttgctgataa
     3541 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3601 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3661 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagacgaagg
     3721 gaaataaata gtagcccgcc tgatgtgcgg gctttttttt ggtggtggtt accagtgtcg
     3781 cttagctacc gtaggtcctc ttaggcggtc cgattagcga catagtcatc aatgccttga
     3841 cggcaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctactaactc tttttccgaa
     4021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgctcac agcccagctt
     4261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4561 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4621 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4681 agagcgccca cctgcaacgc cggcgtaata tggcgcgcca gctggcttgt ggggaccaga
     4741 caaaaaagga atggtgcaga attgttaggc gcacctacca aaagcatctt tgcctttatt
     4801 gcaaagataa agcagattcc tctagtacaa gtggggaaca aaataacgtg gaaaagagct
     4861 gtcctgacag cccactcact aatgcgtatg acgaacgcag tgacgaccac aaaacagctg
     4921 gcttgtgggg accagacaaa aaaggaatgg tgcagaattg ttaggcgcac ctaccaaaag
     4981 catctttgcc tttattgcaa agataaagca gattcctcta gtacaagtgg ggaacaaaat
     5041 aacgtggaaa agagctgtcc tgacagccca ctcactaatg cgtatgacga acgcagtgac
     5101 gaccacaaaa gaattccctc tatataagaa ggcattcatt cccatttgaa ggatcatcag
     5161 atactgaacc aatatttctc actctaagaa attaagagct ttgtattctt caatgagagg
     5221 ctaagacctg caggaaaaat ggcttcctcc
//
