LOCUS       sequence-202173-        7106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Kennedy G, Sugden B
  TITLE     EBNA-1, a bifunctional transcriptional activator.
  JOURNAL   Mol Cell Biol. 2003 Oct;23(19):6901-8.
  PUBMED    12972608
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2003-10"; volume: "23(19)"; pages: "6901-8"
COMMENT     Sequence Label: pCMMP-MCS-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..7106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        85..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    814..1171
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1236..1652
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1582..1604
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1620..1636
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1911..2477
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1927..2479
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2071..2088)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2298..2317
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2491..2493,2494..2496,2497..3210)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2536..2557)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2797..2816)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3144..3165
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4487..4503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4487..4503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4500..4522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4511..4527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4535..4541,4542..4559,4560..4565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4580..4601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4718..4735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4889..5477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4969..4988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5648..6439,6440..6508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6271..6290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6509..6613)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6681..6699
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6737..6759)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6859..6878
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7072..7094
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7086..7103
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7087..7103
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccggcgc cagtcctccg atagactgcg tcgcccgggt acccgtgttc tcaataaacc
      661 ctcttgcagt tgcatccgac tcgtggtctc gctgttcctt gggagggtct cctctgagtg
      721 attgactacc cgtcagcggg gtctttcatt tggaggttcc accgagattt ggagacccct
      781 gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcaactt atctgtgtct
      841 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
      901 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa
      961 ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtcctaaa
     1021 atcccgatcg tttaggactc tttggtgcac cccccttaga ggagggatat gtggttctgg
     1081 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg
     1141 accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga
     1201 ctgtgtttct gtatttgtct gaaaatatgg gcccgggcta gactgttacc actcccttaa
     1261 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1321 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1381 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1441 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1501 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1561 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1621 tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg
     1681 gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc
     1741 cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc
     1801 tggcggcagc ctaccaagaa caactggacc gaccggtcaa ttggttaacg ctgagcttcg
     1861 aagccggcgc ggccgcacgc gtatgcatgt atacctcgag gatccggaat tccgcccccc
     1921 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     1981 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2041 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2101 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2161 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2221 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     2281 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     2341 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     2401 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     2461 gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc
     2521 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2581 tctggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2641 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     2701 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2761 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2821 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2881 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2941 gtctatatca tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     3001 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3061 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     3121 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3181 actctcggca tggacgagct gtacaagtaa tgaattaatt aagaattccg gattagtcca
     3241 atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
     3301 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
     3361 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     3421 ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca
     3481 gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3541 cagggccaag aacagttgga acagcagaat atgggccaaa caggatatct gtggtaagca
     3601 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc
     3661 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3721 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3781 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg
     3841 tcgcccgggt acccgtgttc tcaataaacc ctcttgcagt tgcatccgac tcgtggtctc
     3901 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcag
     3961 tttctcccac ctacacaggt ctcactaaca ttcctgatgt gccgcaggga ctccgtcagc
     4021 ccggtttttg tttataataa aatgcaagaa cagtgttccc ttcaagccag actacatcct
     4081 gactctcggc tttataaaag aatgttgaag ggctctgtgg actatctgcc acacgacttt
     4141 taagattttt atgcctcctg gatgagggat ttagtcaatc tatcctcgtc tattttgctg
     4201 gcttctccgt attttaaatt tctagtttgc actcccttcc tgagagcacg gcgattgcag
     4261 agtagttaat actctgaggg caggcttctg tgaaaaggtt gcctgggctc agtgtgagat
     4321 tttgccataa aaaggggtcc tgcccctgtg tacagacaga tcggaatcta gagtgcatac
     4381 tcagagtccc cgcggttccg gggctctgat ctcagggcat ctttgcctag agatcctcta
     4441 cgccggacgc atcgtggccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
     4501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     6721 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6781 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6841 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     6901 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     6961 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     7021 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     7081 gacgttgtaa aacgacggcc agtgcc
//
