LOCUS       sequence-202174-       11717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hnRNPA1(186-320) fused to mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP
  TITLE     Spatiotemporal Control of Intracellular Phase Transitions Using 
            Light-Activated optoDroplets.
  JOURNAL   Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29.
  PUBMED    28041848
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2017-01-12"; volume: "168(1-2):159-171.e14. doi"; pages: " 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29"
COMMENT     Sequence Label: pHR-HNRNPA1C-mCh-Cry2WT
FEATURES             Location/Qualifiers
     source          1..11717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1028..1039
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1929..3422
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3574..4162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3627..3647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4045..4056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4165..4181)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4166..4182)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4272..4505
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4530..4548)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4531..4548)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4838..4857)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5071..5090)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5190..5212
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5250..5268)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5336..5440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5441..5509,5510..6301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5659..5678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6472..7060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6961..6980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7214..7231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7301..7321)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7306..7635
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7486..7621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8769..8834
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8964..8984
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9409..9543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9446..9465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9500..9519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9711..10344
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10391..10516
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11013..11246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11431..11475
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggct agtgcttcat ccagccaaag aggtcgaagt ggttctggaa
      841 actttggtgg tggtcgtgga ggtggtttcg gtgggaatga caacttcggt cgtggaggaa
      901 acttcagtgg tcgtggtggc tttggtggca gccgtggtgg tggtggatat ggtggcagtg
      961 gggatggcta taatggattt ggtaatgatg gaagcaattt tggaggtggt ggaagctaca
     1021 atgattttgg gaattacaac aatcagtctt caaattttgg acccatgaag ggaggaaatt
     1081 ttggaggcag aagctctggc ccctatggcg gtggaggcca atactttgca aaaccacgaa
     1141 accaaggtgg ctatggcggt tccagcagca gcagtagcta tggcagtggc agaagatttg
     1201 gtggaggaat ggtgtctaaa ggcgaggagg ataacatggc tataatcaag gaatttatga
     1261 ggttcaaggt gcatatggag ggatctgtga acggtcacga gttcgaaatc gagggcgaag
     1321 gcgaggggcg accctatgaa gggacacaga cggccaaact gaaggtgacc aagggtggcc
     1381 ccctgccttt cgcctgggac atcctgtcac cccagtttat gtacggctct aaggcttacg
     1441 ttaagcaccc tgccgatatt ccggactact tgaaactgag cttccctgaa gggttcaagt
     1501 gggaacgggt aatgaatttt gaggatggcg gtgtagtcac agttacccag gacagctctc
     1561 ttcaggacgg agaatttatc tataaggtta aacttcgggg cactaacttc ccatcagacg
     1621 gccccgtgat gcagaagaaa actatgggct gggaagccag ctcagaacgc atgtatcccg
     1681 aggacggagc actgaaggga gaaatcaagc agcgactcaa gctgaaggat gggggacatt
     1741 atgatgctga ggtcaaaacc acctataagg ccaagaaacc cgttcagctt cctggggcat
     1801 acaacgtgaa tatcaaactg gatattacat cccataacga agactatacc atcgtggaac
     1861 agtacgagcg ggccgagggc agacatagca cagggggcat ggatgaattg tacaaggcgg
     1921 ccacgcgtat gaagatggac aaaaagacta tagtttggtt tagaagagac ctaaggattg
     1981 aggataatcc tgcattagca gcagctgctc acgaaggatc tgtttttcct gtcttcattt
     2041 ggtgtcctga agaagaagga cagttttatc ctggaagagc ttcaagatgg tggatgaaac
     2101 aatcacttgc tcacttatct caatccttga aggctcttgg atctgacctc actttaatca
     2161 aaacccacaa cacgatttca gcgatcttgg attgtatccg cgttaccggt gctacaaaag
     2221 tcgtctttaa ccacctctat gatcctgttt cgttagttcg ggaccatacc gtaaaggaga
     2281 agctggtgga acgtgggatc tctgtgcaaa gctacaatgg agatctattg tatgaaccgt
     2341 gggagatata ctgcgaaaag ggcaaacctt ttacgagttt caattcttac tggaagaaat
     2401 gcttagatat gtcgattgaa tccgttatgc ttcctcctcc ttggcggttg atgccaataa
     2461 ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga actagggctg gagaatgagg
     2521 ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc tccaggatgg agcaatgctg
     2581 ataagttact aaatgagttc atcgagaagc agttgataga ttatgcaaag aacagcaaga
     2641 aagttgttgg gaattctact tcactacttt ctccgtatct ccatttcggg gaaataagcg
     2701 tcagacacgt tttccagtgt gcccggatga aacaaattat atgggcaaga gataagaaca
     2761 gtgaaggaga agaaagtgca gatctttttc ttaggggaat cggtttaaga gagtattctc
     2821 ggtatatatg tttcaacttc ccgtttactc acgagcaatc gttgttgagt catcttcggt
     2881 ttttcccttg ggatgctgat gttgataagt tcaaggcctg gagacaaggc aggaccggtt
     2941 atccgttggt ggatgccgga atgagagagc tttgggctac cggatggatg cataacagaa
     3001 taagagtgat tgtttcaagc tttgctgtga agtttcttct ccttccatgg aaatggggaa
     3061 tgaagtattt ctgggataca cttttggatg ctgatttgga atgtgacatc cttggctggc
     3121 agtatatctc tgggagtatc cccgatggcc acgagcttga tcgcttggac aatcccgcgt
     3181 tacaaggcgc caaatatgac ccagaaggtg agtacataag gcaatggctt cccgagcttg
     3241 cgagattgcc aactgaatgg atccatcatc catgggacgc tcctttaacc gtactcaaag
     3301 cttctggtgt ggaactcgga acaaactatg cgaaacccat tgtagacatc gacacagctc
     3361 gtgagctact agctaaagct atttcaagaa cccgtgaagc acagatcatg atcggagcag
     3421 cagcccggga tccaccggtc gccaccggtt cagggagcgg atccggctca gggagtgcgg
     3481 ccgcaactcc cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg
     3541 caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     3601 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3661 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3721 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3781 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3841 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3961 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4021 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4081 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4141 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta attcgagctc
     4201 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4261 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     4321 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4501 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     4561 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     4621 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     4681 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     4741 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     4801 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     4861 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     4921 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     4981 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     5041 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5101 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5161 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5221 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5281 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5341 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5401 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5461 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5521 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5581 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5641 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5701 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5761 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5821 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5881 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5941 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6001 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6061 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6121 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6181 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6241 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6301 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6361 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6421 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6481 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6541 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6601 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6661 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6721 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6781 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6841 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6901 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7021 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7081 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7141 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7201 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7261 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
     7321 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7381 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7441 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7501 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7561 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7621 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
     7681 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     7741 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     7801 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     7861 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     7921 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
     7981 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
     8041 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
     8101 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
     8161 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
     8221 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     8281 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     8341 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     8401 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     8461 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
     8521 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
     8581 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
     8641 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
     8701 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     8761 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     8821 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     8881 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     8941 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     9001 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     9061 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     9121 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     9181 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     9241 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     9301 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     9361 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     9421 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9481 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9541 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     9601 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     9661 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     9721 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     9781 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     9841 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     9901 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     9961 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
    10021 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
    10081 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
    10141 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10321 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
    10381 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
    10441 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
    10501 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
    10561 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
    10621 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
    10681 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
    10741 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
    10801 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
    10861 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
    10921 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    10981 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    11041 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    11101 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    11161 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    11221 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    11281 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    11341 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    11401 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    11461 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    11521 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    11581 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    11641 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    11701 tccattcgat tagtgaa
//
