LOCUS       sequence-20219-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKgfp1
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(107..127)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctagtggatc ccccgggctg caggaattcg atatcaagct tgcatgcctg caggtctgga
       61 catttatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
      121 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      181 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      241 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      301 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      361 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      421 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      481 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      541 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      601 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      661 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      721 accatctaat
//
