LOCUS       sequence-202190-        8855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, 
            Feldman JL, Perrimon N, Ting AY
  TITLE     Efficient proximity labeling in living cells and organisms with 
            TurboID.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
  PUBMED    30125270
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201."
COMMENT     Sequence Label: C1(1-29)-TurboID-V5_pLX304
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             796..1752
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1759..1800
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(1805..1829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1862..2450
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1915..1935)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2333..2344)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2522..2755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2833..2954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2870..2889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2924..2943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2994..3129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3149..3168)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3150..3168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3178..3195)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3178..3194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3187..3209)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3336..3791
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3423..3442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3633..3654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3817..3921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3922..3990,3991..4782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4953..5541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5442..5461
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5695..5712
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5829..5850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5865..5870,5871..5888,5889..5895)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5903..5919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5908..5930
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5927..5943
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5927..5943
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5962..5982
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5964..5982
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6010..6236
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6237..6417
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6464..6589
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7082..7315
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7500..7544
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7719..8219
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8138..8156
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8231..8626
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8686..8803
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg accctgtggt cgtgctgggg
      661 ctgtgcctga gctgtctgct gctgctgagc ctgtggaagc agtcctacgg aggcggggga
      721 tcaggaagcg gcggccgcgg atcaggaagc ggatcaggaa gcggatcagg aagcggatca
      781 ggaagcggat caggaaaaga caatactgtg cctctgaagc tgatcgctct cctggctaat
      841 ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag ggccgctatc
      901 aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt gcccggaaag
      961 ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct gggacagctg
     1021 gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat
     1081 cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga
     1141 ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt
     1201 tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg
     1261 gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg
     1321 tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat
     1381 gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc
     1441 gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact
     1501 ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc
     1561 ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag acccgtgaag
     1621 ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc
     1681 ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg
     1741 tctgccgaaa aggctagcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     1801 taagacccag ctttcttgta caaagtggtg ctagcgctaa ccggtggcgc gttaagtcga
     1861 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1921 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1981 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2041 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2101 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2161 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2221 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2281 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2341 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2401 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     2461 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     2521 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     2581 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2641 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     2701 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     2761 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     2821 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2941 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     3001 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3061 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3121 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     3181 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3241 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3301 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     3361 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3421 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3481 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3541 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3601 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3661 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3721 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3781 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3841 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3901 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3961 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4021 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4081 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4141 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4201 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4261 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4321 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4381 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4441 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4501 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4561 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4621 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4681 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4741 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4801 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4861 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5341 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5581 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5641 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5701 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5761 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5821 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     5881 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     5941 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6001 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     6061 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     6121 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     6181 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     6241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     6421 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     6481 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     6541 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     6601 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     6661 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     6721 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     6781 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     6841 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     6901 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     6961 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7021 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7081 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     7141 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     7201 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     7261 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     7321 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     7381 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     7441 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     7501 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     7561 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     7621 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     7681 tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc
     7741 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     7801 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     7861 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     7921 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     7981 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     8041 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     8101 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     8161 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     8221 gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     8281 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     8341 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     8401 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     8461 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     8521 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     8581 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta
     8641 gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     8701 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     8761 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     8821 cagcagagat ccactttggc gccggctcga ggggg
//
