LOCUS       sequence-202192-        8669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses V5-tagged miniTurbo on the mammalian ER membrane, 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, 
            Feldman JL, Perrimon N, Ting AY
  TITLE     Efficient proximity labeling in living cells and organisms with 
            TurboID.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
  PUBMED    30125270
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201."
COMMENT     Sequence Label: C1(1-29)-miniTurbo-V5_pLX304
FEATURES             Location/Qualifiers
     source          1..8669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             796..1566
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1573..1614
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(1619..1643)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1676..2264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1729..1749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2147..2158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2336..2569
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2647..2768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2684..2703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2738..2757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2808..2943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2963..2982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2964..2982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2992..3009)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2992..3008)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3001..3023)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3150..3605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3237..3256)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3447..3468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3631..3735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3736..3804,3805..4596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4767..5355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5256..5275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5509..5526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5643..5664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5679..5684,5685..5702,5703..5709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5717..5733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5722..5744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5741..5757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5741..5757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5776..5796
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5778..5796
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5824..6050
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6051..6231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6278..6403
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6896..7129
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7314..7358
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7533..8033
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7952..7970
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8045..8440
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8500..8617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg accctgtggt cgtgctgggg
      661 ctgtgcctga gctgtctgct gctgctgagc ctgtggaagc agtcctacgg aggcggggga
      721 tcaggaagcg gcggccgcgg atcaggaagc ggatcaggaa gcggatcagg aagcggatca
      781 ggaagcggat caggaatccc gctgctgaac gctaaacaga ttctgggaca gctggacggc
      841 gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct ggatcgaatc
      901 ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg gagaggaagc
      961 agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat gttttggcgc
     1021 ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt catggcagaa
     1081 gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga cctgtatctg
     1141 caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg cgatgctgca
     1201 cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga aagcgtggtc
     1261 aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa tactctggcc
     1321 gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga aggcctggct
     1381 ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt gaagctgatc
     1441 attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg agccctgctg
     1501 ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct gcggtctgcc
     1561 gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag cacctaagac
     1621 ccagctttct tgtacaaagt ggtgctagcg ctaaccggtg gcgcgttaag tcgacaatca
     1681 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1741 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1801 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1861 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1921 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1981 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2041 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2101 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2161 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2221 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     2281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2341 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     2401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     2581 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     2641 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2701 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2761 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     2821 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2881 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2941 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3001 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3061 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3121 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3361 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3481 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3601 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3661 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3721 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3781 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3841 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3901 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3961 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4021 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4081 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4141 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4201 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4261 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4321 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4381 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4441 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4501 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4561 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4621 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4681 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4741 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5341 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5401 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5461 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5521 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5581 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5641 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5701 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     5761 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     5821 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     5881 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     5941 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     6001 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6241 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6301 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6361 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6421 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6481 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6541 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6601 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6661 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6721 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     6781 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     6841 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     6901 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     6961 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7021 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7081 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7141 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7201 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7261 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7321 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7381 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7441 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7501 ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt
     7561 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     7621 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     7681 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     7741 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     7801 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     7861 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     7921 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     7981 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac
     8041 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     8101 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     8161 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     8221 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     8281 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     8341 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     8401 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga
     8461 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8581 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     8641 agatccactt tggcgccggc tcgaggggg
//
