LOCUS       sequence-202198-        9890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian
            cells, with an Igk-leader. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Pratt CP, He J, Wang Y, Barth AL, Bruchez MP
  TITLE     Fluorogenic Green-Inside Red-Outside (GIRO) Labeling Approach 
            Reveals Adenylyl Cyclase-Dependent Control of BKalpha Surface 
            Expression.
  JOURNAL   Bioconjug Chem. 2015 Sep 16;26(9):1963-71. doi: 
            10.1021/acs.bioconjchem.5b00409. Epub 2015 Sep 2.
  PUBMED    26301573
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.bioconjchem.5b00409"; journalName: "Bioconjug Chem"; 
            date: "2015-09-16- 16"; volume: "26"; issue: "9"; pages: "1963-71"
COMMENT     Sequence Label: pcDNA3.1-kappa-HA-dL5-BKa
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        110..491
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        492..695
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     646..666
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        740..758
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     824..883
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             887..913
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             929..1648
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
     polyA_signal    5122..5256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5356..5373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5362..5586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5632..6060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5719..5738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5929..5950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6069..6089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6074..6403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6254..6389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6316..6335
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6470..7264
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6524..6543)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7134..7153
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7438..7559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7608..7624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7608..7624)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7621..7643)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7632..7648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7656..7662,7663..7680,7681..7686))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7701..7722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7839..7856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8010..8598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8090..8109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8769..9560,9561..9629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9392..9411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9630..9734)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9809..9828)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
       61 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      121 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      181 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      361 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      421 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      481 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      541 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      601 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      661 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
      721 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt
      781 taaacttaag cttgccaccg gatccggctt ggggatatcc accatggaga cagacacact
      841 cctgctatgg gtactgctgc tctgggttcc aggttccact ggtgactatc catatgatgt
      901 tccagattat gctggggccc agccggccca ggccgtcgtt acccaagaac ctagtgttac
      961 cgttagccca ggtggtactg ttatacttac ttgtggaagt ggtacgggtg ccgtcacatc
     1021 tggtcattat gcaaattggt ttcaacaaaa accaggacaa gctccaagag ctttgatttt
     1081 tgatactgat aagaagtatt cttggacccc aggtagattt tctggatctt tgctgggagc
     1141 aaaggcagct ttgacaatat cagatgctca gcctgaggac gaagccgagt attactgttc
     1201 tcttagcgac gtggatggct acttgtttgg cggtggaaca caactgacgg ttctgtccgg
     1261 tggtggcggc tctggtggcg gtggcagcgg cggtggtggt tccggaggcg gcggttctca
     1321 ggctgtggtg actcaggagc cgtcagtgac tgtgtcccca ggagggacag tcattctcac
     1381 ttgtggctcc ggcactggag ctgtcaccag tggtcattat gccaactggt tccagcagaa
     1441 gcctggccaa gcccccaggg cacttatatt tgacaccgac aagaagtatt cctggacccc
     1501 tggccgattc tcaggctccc tccttggggc caaggctgcc ctgaccatct cggatgcgca
     1561 gcctgaagat gaggctgagt attactgttc gctctccgac gttgacggtt atctgttcgg
     1621 aggaggcacc cagctgaccg tcctctccac tagtatggat gcgctcatca taccggtgac
     1681 catggaggtg ccgtgcgaca gccggggcca acgcatgtgg tgggctttct tggcctcctc
     1741 catggtgact ttcttcgggg gcctcttcat catcttgctc tggcggacgc tcaagtacct
     1801 gtggaccgtt tgctgccact gcgggggcaa gacgaaggag gcccagaaga taaacaatgg
     1861 ctccagccag gcagatggta ctctcaagcc agtggacgaa aaagaggagg tggtggcagc
     1921 cgaggtcggc tggatgacat ctgtgaagga ctgggcaggg gtgatgatat ccgcccagac
     1981 actgactggc agagtcctgg ttgtgttagt ctttgctctc agcattggtg ccctcgtaat
     2041 atacttcata gactcgtcaa acccaataga atcctgccag aatttctaca aagatttcac
     2101 attacagatc gacatggctt tcaacgtgtt cttcctcctc tactttggct tgcggtttat
     2161 tgcagccaac gataagctgt ggttctggct ggaagtgaat tcagtagtag atttcttcac
     2221 agtccctcct gtgtttgtgt ctgtgtactt aaacagaagt tggcttggct tgagattttt
     2281 aagagctctc agactgatac agttttcaga gattttgcag tttctgaata tccttaaaac
     2341 aagtaactcc atcaagctgg tgaatctgct ctccatattt atcagcacgt ggctgactgc
     2401 agctggattc atccacttgg tggagaattc aggggaccca tgggaaaatt tccaaaacaa
     2461 ccaggcactt acgtactggg aatgtgtcta cttactcatg gtcacaatgt ctacagtggg
     2521 ttatggggac gtttatgcaa aaaccacact tggacgcctc ttcatggtct tcttcatcct
     2581 cgggggactg gccatgtttg ccagctacgt ccctgaaatc atagagttaa taggaaaccg
     2641 caagaaatac gggggctcct atagcgcggt tagtggaaga aagcacattg tagtctgtgg
     2701 acacattact ctggagagtg tctctaactt cctgaaggac tttctgcaca aggaccggga
     2761 tgatgtcaac gtggagattg tctttcttca caacatctcc cctaaccttg agcttgaggc
     2821 tctgttcaaa cggcatttca ctcaggtgga gttttatcag ggctctgtcc tcaatccaca
     2881 tgatcttgcc agagtcaaga tagagtcagc agatgcatgc ctgatccttg ccaataagta
     2941 ttgcgctgac ccggatgcag aagatgcctc caacatcatg agagtgatct ccatcaaaaa
     3001 ctaccaccca aagatcagga tcatcactca gatgctgcag tatcacaaca aggcccatct
     3061 gctcaacatc cccagctgga actggaaaga gggtgatgac gcaatatgcc ttgcagagct
     3121 caagttgggt ttcatagccc agagctgtct ggctcaaggc ctctccacaa tgcttgccaa
     3181 tctcttctct atgaggtcat tcataaagat tgaggaagac acatggcaga aatactactt
     3241 ggaaggagtc tccaatgaaa tgtacacaga atatctctcc agtgccttcg tgggtctgtc
     3301 cttccctact gtttgtgagc tgtgttttgt gaagcttaag ctcctgatga tagccattga
     3361 gtacaagtct gccaacagag agagccgaag ccgaaagcga atattaatta accctgggaa
     3421 ccaccttaag atccaagaag gtactttagg atttttcatc gcaagtgatg ccaaagaagt
     3481 taaaagggca tttttttact gcaaggcctg tcatgatgac gtcacagatc ccaaaagaat
     3541 taaaaaatgt ggctgcaggc ggcttgaaga tgagcagccg ccaaccctgt caccaaaaaa
     3601 aaaacaacgt aatgggggca tgaggaactc gcccaacacc tccccgaagc tgatgaggca
     3661 tgaccccttg ttaattcctg gcaatgatca gattgacaac atggactcca atgtgaaaaa
     3721 gtacgactcc actggaatgt ttcactggtg tgcacccaag gagattgaga aagtcatctt
     3781 gactcgaagt gaagctgcca tgactgtcct gagtggccat gtcgtagtct gcatctttgg
     3841 ggatgtcagc tcagccctga ttggcctccg gaacctggtg atgccacttc gtgctagcaa
     3901 ctttcactat catgagctca aacacattgt gtttgtgggc tccattgagt acctcaagag
     3961 ggagtgggaa acactgcaca acttcccgaa agtgtccata ttgcctggta caccattaag
     4021 tcgggctgat ttaagggctg tcaacatcaa cctctgtgac atgtgcgtta tcctgtcagc
     4081 caatcagaat aatattgatg atacttcgct tcaggacaag gaatgcatct tggcgtcact
     4141 caacatcaaa tctatgcagt ttgatgacag catcggggtc ttgcaggcta attcccaagg
     4201 attcacacct cctggaatgg acagatcatc acccgacaac agcccagtgc acgggatgtt
     4261 acgccagccg tccatcacaa ctggggtcaa cattcccatc atcacggaac tcgctaagcc
     4321 gggcaagttg cctttggtat cagtcaatca ggaaaaaaac agtgggacgc acattctaat
     4381 gataacggag ttggtgaatg ataccaatgt tcagtttttg gaccaagacg atgacgatga
     4441 ccctgacaca gagctgtacc tcacacagcc ctttgcttgt gggacagcat ttgccgtcag
     4501 cgtcctggac tcactcatga gcgcgacata cttcaatgac aatatcctca ccctaatacg
     4561 gaccctggtg acaggaggag ccacaccaga gctcgaggct ctaatagctg aggagaatgc
     4621 acttcgagga ggctacagca ctccgcagac attggccaac agggaccgtt gccgagtggc
     4681 ccagttagcc ctgttagatg gtccctttgc agacttaggg gatggtggtt gttatggtga
     4741 tctgttctgc aaagctctga aaacatataa tatgctttgt tttggaattt accggctgag
     4801 agatgcccac ctcagcaccc ccagccagtg tacaaaaagg tacgtcatca ccaatcctcc
     4861 ctacgagttt gagctcgtac caacagacct gatcttctgc ctgatgcagt ttgaccacaa
     4921 cgctggccaa tcccgggcca gtctgtctca ttcctcccac tcctcacagt cgtccagtaa
     4981 gaagagctcc tccgtccact ccatcccgtc cacagcaaat cggccgaacc ggcccaagtc
     5041 cagggagtcc cgcgacaaac agaacagaaa agaaatggtt tacagataga tcgatagaac
     5101 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
     5161 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5221 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattc ggaggttctc
     5281 atatgggcgg gtcaaccggt atcttactcg aggcagctgt cgactctaga gggcccgttt
     5341 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5401 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5461 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5521 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5581 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     5641 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5701 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5761 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5821 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5881 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5941 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6001 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6061 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     6361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     6421 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     6481 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6541 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6601 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6661 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6721 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6781 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6841 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6901 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6961 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     7021 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7081 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7141 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7201 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7261 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     7321 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7381 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     7441 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7501 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7561 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7621 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7681 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7741 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7801 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7861 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8821 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8881 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8941 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9001 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9061 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9121 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9181 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9241 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9301 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9361 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9421 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9481 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9541 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9601 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9661 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9721 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     9781 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     9841 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
//
