LOCUS       sequence-202203-        9898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Confers constitutive expression of KKH SaCas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Shen MW, Arbab M, Hsu JY, Worstell D, Culbertson SJ, Krabbe O, Cassa
            CA, Liu DR, Gifford DK, Sherwood RI
  TITLE     Predictable and precise template-free CRISPR editing of pathogenic 
            variants.
  JOURNAL   Nature. 2018 Nov;563(7733):646-651. doi: 10.1038/s41586-018-0686-x. 
            Epub 2018 Nov 7.
  PUBMED    30405244
REFERENCE   2  (bases 1 to 9898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0686-x"; journalName: "Nature"; date: "2018-11";
            volume: "563"; issue: "7733"; pages: "646-651"
COMMENT     Sequence Label: p2T-CAG-KKHSaCas9-BlastR
FEATURES             Location/Qualifiers
     source          1..9898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..853)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      878..887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(893..926)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        934..1313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1316..1591
                     /label=chicken beta-actin promoter
     intron          1592..2600
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2524..2546
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2608..2627
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2693..2702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5867..5887
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5894..5959
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6019..6140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6323..6444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6602..7000)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(7019..7066)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7114..7443)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7128..7263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7182..7201)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7428..7448
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7458..7645,7646..7657)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7714..7734)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7714..7732)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7753..7769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7753..7769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7766..7788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7777..7793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7801..7807,7808..7825,7826..7831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7846..7867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7984..8001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8155..8743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8235..8254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8914..9705,9706..9774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9537..9556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9775..9879)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatcga tctcgaagga tctggaggcc accatggtgt cgataacttc
      901 gtatagcata cattatacga agttatcgtg ctcgacattg attattgact agttattaat
      961 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     1021 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     1081 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggact
     1141 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     1201 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     1261 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag
     1321 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     1381 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggcgcgc
     1441 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     1501 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     1561 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt cgccccgtgc
     1621 cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1681 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1741 gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg
     1801 cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     1861 gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1921 cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg
     1981 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg
     2041 cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     2101 tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     2161 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     2221 gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     2281 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tggcggagcc
     2341 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg gtgcggcgcc
     2401 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     2461 catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga cggggcaggg
     2521 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     2581 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2641 ttggcaaaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
     2701 ggggaaacgg aactacatcc tggggcttga cattgggata accagcgttg gctacggaat
     2761 tattgattat gagacacgcg atgtgattga cgccggggtt aggctgttca aagaggccaa
     2821 cgttgaaaac aacgagggaa gacggagtaa gcgcggagca agaagactca agcgcagacg
     2881 gagacatcgg attcagaggg tgaaaaagct gctcttcgat tacaatctcc tgaccgatca
     2941 tagtgagctg agcggaatca acccctacga ggcgcgagtg aaagggcttt cccagaagct
     3001 gtccgaagag gagttctccg ccgcgttgct gcacctggcc aaacggaggg gggttcacaa
     3061 tgtaaacgaa gtggaggagg acacgggcaa tgaacttagt acgaaagaac agatcagtag
     3121 gaactctaag gctctcgaag agaaatacgt cgctgagttg cagcttgaga gactgaaaaa
     3181 agacggcgaa gtacgcggat ctattaatag gttcaagact tcagattacg taaaggaagc
     3241 caagcagctc ctgaaagtac agaaagcgta ccatcagctc gatcagagct tcatcgatac
     3301 ctacatagat ttgctggaga cacggaggac atactacgag ggcccagggg aaggatctcc
     3361 ttttgggtgg aaggacatca aggaatggta cgagatgctt atgggacatt gtacatattt
     3421 tccggaggag ctcaggagcg tcaagtacgc ctacaatgcc gacctgtaca atgccctcaa
     3481 tgacctcaat aacctcgtga ttaccaggga cgagaacgag aagctggagt actatgaaaa
     3541 gttccagatt atcgagaatg tgtttaagca gaagaagaag ccgacactta agcagattgc
     3601 aaaggaaatc ctcgtgaatg aggaagatat caagggatac agagtgacaa gtacaggcaa
     3661 gcccgagttc acaaatctga aggtgtacca cgatattaag gacataaccg cacgaaagga
     3721 gataatcgaa aacgctgagc tcctcgatca gatcgcaaaa attcttacca tctaccagtc
     3781 tagtgaggac attcaggagg aactgactaa tctgaacagt gagctcaccc aagaggaaat
     3841 tgagcagatt tcaaacctga aaggctacac cgggacgcac aatctgagcc tcaaagcaat
     3901 caacctcatt ctggatgaac tttggcacac aaatgacaac caaattgcca tattcaaccg
     3961 cctgaaactg gtgccaaaaa aagtggatct gtcacagcaa aaggaaatcc ctacaacctt
     4021 ggttgacgat tttattctgt cccccgttgt caagcggagc ttcatccagt caatcaaggt
     4081 gatcaatgcc atcattaaaa aatacggatt gccaaacgat ataattatcg agcttgcacg
     4141 agagaagaac tcaaaggacg cccagaagat gattaacgaa atgcagaagc gcaaccgcca
     4201 gacaaacgaa cgcatagagg aaattataag aacaaccggc aaagagaatg ccaagtatct
     4261 gatcgagaaa atcaagctgc acgacatgca agaaggcaag tgcctgtact ctctggaagc
     4321 tatcccactc gaagatctgc tgaataatcc attcaattac gaggtggacc acatcatccc
     4381 tagatccgta agctttgaca attccttcaa taacaaagtt ctggttaaac aggaggaaaa
     4441 ttctaaaaaa gggaaccgga ccccgttcca gtacctgagc tccagtgaca gcaagattag
     4501 ctacgagact tttaagaaac atattctgaa tctggccaaa ggcaaaggca ggatcagcaa
     4561 gaccaagaag gagtacctcc tcgaagaacg cgacattaac agatttagtg tgcagaaaga
     4621 tttcatcaac cgaaaccttg tcgatactcg gtacgccacg agaggcctga tgaatctcct
     4681 caggagctac ttccgcgtca ataatctgga cgttaaagtc aagagcataa atgggggatt
     4741 caccagcttt ctgaggagaa agtggaagtt taagaaggaa cgaaacaaag gatacaagca
     4801 ccatgctgag gatgctttga tcatcgctaa cgcggacttt atctttaagg aatggaaaaa
     4861 gctggataag gcaaagaaag tgatggaaaa ccagatgttc gaggagaagc aggcagagtc
     4921 aatgcctgag atcgagacag agcaggaata caaggaaatt ttcatcaccc ctcatcagat
     4981 taaacacata aaggacttca aagactataa atactctcat agggtggaca aaaaacccaa
     5041 tcgcaagctc attaatgaca ccctgtactc aacacggaag gatgataaag gtaatacctt
     5101 gattgtgaat aatcttaatg gattgtatga caaagataac gacaagctca agaagctgat
     5161 caacaagtct ccagagaagc tccttatgta tcaccacgac ccacagactt atcagaaatt
     5221 gaaactgatc atggagcaat acggggatga gaagaaccca ctctacaaat attatgagga
     5281 aacaggtaat tacctgacca agtactccaa gaaggataac ggaccagtga tcaaaaagat
     5341 aaagtactat ggcaacaaac ttaatgcgca tttggacata actgacgatt accccaattc
     5401 tcgaaacaag gttgtgaagc tctccctgaa gccttataga tttgacgtgt acctggataa
     5461 tggggtttat aaattcgtca ccgtgaaaaa tctggacgtg atcaaaaagg agaactatta
     5521 tgaagtaaac tcaaagtgct atgaggaggc gaagaagctg aagaagatct ccaatcaggc
     5581 cgagttcatc gcttccttct ataagaacga tctcatcaag atcaatggag agctttatcg
     5641 cgtcattggt gtgaacaatg acttgctgaa caggatcgaa gtcaatatga tagacattac
     5701 ctaccgggag tatctcgaaa acatgaatga taaacggccg cctcacatca tcaagacaat
     5761 cgcatctaaa actcagtcaa taaaaaagta ctctaccgat atcctgggga atctctatga
     5821 agtgaagtca aagaagcacc cacaaatcat taaaaaaggt ggatccccca agaagaagag
     5881 gaaagtctcg agcgactaca aagaccatga cggtgattat aaagatcatg acatcgatta
     5941 caaggatgac gatgacaagt aaagcggccg caccggtgat atcaagcttg tttaaacggc
     6001 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     6061 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     6121 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     6181 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgcga
     6241 taaggatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6301 gcgcagcctg aaacagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6361 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6421 attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg
     6481 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     6541 ggcggcggtg gaatcgaaat ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg
     6601 cttagccctc ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct
     6661 gtccatcact gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc
     6721 ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag
     6781 tcaggttgcc agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc
     6841 ccccagtaaa atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg
     6901 cgctggcgac gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt
     6961 caatgagggt ggattcttct tgagacaaag gcttggccat ggtttagttc ctcaccttgt
     7021 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcaga
     7081 tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa
     7141 gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat
     7201 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     7261 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     7321 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     7381 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     7441 cacattccac agaattcaag tgatctccaa aaaataagta ctttttgact gtaaataaaa
     7501 ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt
     7561 attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag
     7621 taatcaagta aaattactca agtactttac acctctggtt cttgaccccc taccttcagc
     7681 aagcccagca gatccgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     7741 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7801 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7861 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7921 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7981 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8041 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8101 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8161 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8221 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8281 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8341 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8401 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8461 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8521 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8581 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8641 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8701 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8761 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8821 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8881 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8941 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9001 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9061 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtg
//
