LOCUS       sequence-202213-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman 
            DE
  TITLE     An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves 
            Polycomb Silencing.
  JOURNAL   Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 
            10.1016/j.molcel.2019.03.018.
  PUBMED    30982744
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 
            10.1016/j.molcel.2019.03.018."
COMMENT     Sequence Label: pET30a-ASXL1-HARE-HTH domain
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             55..99
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             355..378
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             388..405
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(458..476)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      472..519
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(643..662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgcaccatc atcatcntcn ttcttctggt ctggtgccac gcggttctgg tatgaaagaa
       61 accgctgctg ctaaattcga acgccagcac atggacagcc cagatctgaa ggacaaacag
      121 aagaggaaga aggagcgcac gtgggccgag gccgcgcgcc tggtgttaga aaactactca
      181 gatgctccaa tgacaccaaa acagattctg caggtcatag aggcagaagg actgaaggaa
      241 atgagaagtg ggacatcccc tcttgcgtgc ctcaatgcca tgctacattc caactcaaga
      301 ggaggagaag ggctgtttta taaattacct ggccgcatta gtcttttcac actcgattac
      361 aaggatgacg atgacaagtg actcgagcac caccaccacc accactgaga tccggctgct
      421 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      481 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      541 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      661 cttcctttct cgccacgttc gccggctttc cccgtcnngc tctaaatcgg gggctccctt
      721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      781 gttcacgtag tgggccatc
//
