LOCUS       sequence-20224-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKlux2.1
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccctcgaggt cgacggtatc gataagcttg atatcgaatt caggcttgga ggatacgtat
       61 gactaaaaaa atttcattca ttattaacgg ccaggttgaa atctttcccg aaggtgatga
      121 tttagtgcaa tccattaatt ttggtgataa tagtgtttac ctgccaatat tgaatgactc
      181 tcatgtaaaa aacattattg attgtaatgg aaataacgaa ttacggttgc ataacattgt
      241 caattttctc tatacggtag ggcaaagatg gaaaaatgaa gaatactcaa gacgcaggac
      301 atacattcgt gacttaaaaa aatatatggg atattcagaa gaaatggcta agctagaggc
      361 caattggata tctatgattt tatgttctaa aggcggcctt tatgatgttg tagaaaatga
      421 acttggttct cgccatatca tggatgaatg gctacctcag gatgaaagtt atgttcgggc
      481 ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat gttccattat ctgggatcat
      541 gtctatatta cgcgcaattt taactaagaa tcagtgtatt ataaaaacat cgtcaaccga
      601 tccttttacc gctaatgcat tagcgttaag ttttattgat gtagacccta atcatccgat
      661 aacgcgctct ttatctgtta tatattggcc ccaccaaggt gatacatcac tcgcaaaaga
      721 aattatgcaa catgcggatg ttattgtcgc ttggg
//
