LOCUS       sequence-20225-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKlux3
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..109)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 atcgatggtg caaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
       61 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcat
//
