LOCUS       sequence-202264-       11584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MEF2C phosphomimetic mutant serine to aspartic acid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pINDUCER20 MEF2C S222D
FEATURES             Location/Qualifiers
     source          1..11584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2728..2737
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4168..4192)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4245..4271
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4357..5568
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5405..5424
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5627..6244,6245..6250,6251..6286,6287..6289,6290..6325,
                     6326..6328,6329..6364,6365..6373)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6411..6985
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6578..6595)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             7001..7795
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7001..7795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7055..7074)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7665..7684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7974..8562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8027..8047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8445..8456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8637..8870
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8989..9007)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9075..9179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9180..9248,9249..10040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9398..9417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10211..10799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10700..10719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10953..10970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11210..11231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11246..11251,11252..11269,11270..11276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11284..11300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11289..11311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11308..11324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11308..11324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11374..11393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctgagcc accatgggga gaaaaaagat tcagattacg
     2761 aggattatgg atgaacgtaa cagacaggtg acatttacaa agaggaaatt tgggttgatg
     2821 aagaaggctt atgagctgag cgtgctgtgt gactgtgaga ttgcgctgat catcttcaac
     2881 agcaccaaca agctgttcca gtatgccagc accgacatgg acaaagtgct tctcaagtac
     2941 acggagtaca acgagccgca tgagagccgg acaaactcag acatcgtgga gacgttgaga
     3001 aagaagggcc ttaatggctg tgacagccca gaccccgatg cggacgattc cgtaggtcac
     3061 agccctgagt ctgaggacaa gtacaggaaa attaacgaag atattgatct aatgatcagc
     3121 aggcaaagat tgtgtgctgt tccacctccc aacttcgaga tgccagtctc catcccagtg
     3181 tccagccaca acagtttggt gtacagcaac cctgtcagct cactgggaaa ccccaaccta
     3241 ttgccactgg ctcacccttc tctgcagagg aatagtatgt ctcctggtgt aacacatcga
     3301 cctccaagtg caggtaacac aggtggtctg atgggtggag acctcacgtc tggtgcaggc
     3361 accagtgcag ggaacgggta tggcaatccc cgaaacgacc caggtctgct ggtctcacct
     3421 ggtaacttga acaagaatat gcaagcaaaa tctcctcccc caatgaattt aggaatgaat
     3481 aaccgtaaac cagatctccg agttcttatt ccaccaggca gcaagaatac gatgccatca
     3541 gtgtctgagg atgtcgacct gcttttgaat caaaggataa ataactccca gtcggctcag
     3601 tcattggcta ccccagtggt ttccgtagca actcctactt taccaggaca aggaatggga
     3661 ggatatccat cagccatttc aacaacatat ggtacggagt actctctgag tagtgcagac
     3721 ctgtcatctc tgtctgggtt taacaccgcc agcgctcttc accttggttc agtaactggc
     3781 tggcaacagc aacacctaca taacatgcca ccatctgccc tcagtcagtt gggagcttgc
     3841 actagcactc atttatctca gagttcaaat ctctccctgc cttctactca aagcctcaac
     3901 atcaagtcag aacctgtttc tcctcctaga gaccgtacca ccaccccttc gagataccca
     3961 caacacacgc gccacgaggc ggggagatct cctgttgaca gcttgagcag ctgtagcagt
     4021 tcgtacgacg ggagcgaccg agaggatcac cggaacgaat tccactcccc cattggactc
     4081 accagacctt cgccggacga aagggaaagt ccctcagtca agcgcatgcg actttctgaa
     4141 ggatgggcaa cataaggatc ccccgggacc cagctttctt gtacaaagtg gttgatatcc
     4201 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaataccca tacgacgtcc
     4261 cagactacgc ttagtaatga ttaattaaac tagaaattct accgggtagg ggaggcgctt
     4321 ttcccaaggc agtctggagt aacccaccca agatctggcc tccgcgccgg gttttggcgc
     4381 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     4441 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     4501 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     4561 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     4621 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     4681 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     4741 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     4801 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     4861 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     4921 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     4981 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     5041 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     5101 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     5161 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     5221 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     5281 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     5341 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     5401 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     5461 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     5521 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac
     5581 cgagctcgga tctccacccc gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga
     5641 caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct
     5701 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     5761 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     5821 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     5881 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     5941 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     6001 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     6061 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     6121 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     6181 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     6241 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     6301 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     6361 gctccccggg taactaagta aggatccgcg gccgcactag aggaattccg cccctctccc
     6421 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgtgtttgtc
     6481 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6541 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6601 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6661 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6721 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6781 tggatagttg tggaaagagt caaatggctc tcctcaagcg tagtcaacaa ggggctgaag
     6841 gatgcccaga aggtacccca ttgtatggga atctgatctg gggcctcggt gcacatgctt
     6901 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     6961 tttcctttga aaaacacgat gataagctta ccggtccacc atgattgaac aagatggatt
     7021 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7081 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7141 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     7201 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7261 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7321 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7381 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7441 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7501 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     7561 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7621 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     7681 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     7741 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgatgtac
     7801 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     7861 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     7921 ttgttgttta gtccctccca attcgatatc aagcttatcg atcgatagat cctaatcaac
     7981 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     8041 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     8101 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8161 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     8221 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     8281 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     8341 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     8401 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     8461 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     8521 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     8581 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8641 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8701 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8761 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8821 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8881 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8941 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     9001 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     9061 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9121 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9181 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9241 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9301 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9361 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9421 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9481 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9541 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9601 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9661 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9721 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9781 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9841 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9901 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9961 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10021 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10081 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10141 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10321 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10441 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10621 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10861 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10921 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10981 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    11041 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    11101 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    11161 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11221 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    11281 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    11341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11401 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    11461 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    11521 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11581 tagt
//
