LOCUS       sequence-202265-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gene expression vector for phospho-MARK3 expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pLX304-MARK3
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2844..2868)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2870..2911
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2953..3541
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3006..3026)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3424..3435)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3613..3846
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3924..4045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3961..3980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4015..4034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4085..4220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4240..4259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4241..4259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4269..4286)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4269..4285)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4278..4300)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4427..4882
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4514..4533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4724..4745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4908..5012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5013..5081,5082..5873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5231..5250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6044..6632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6533..6552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6786..6803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6920..6941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6956..6961,6962..6979,6980..6986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6994..7010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6999..7021
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7018..7034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7018..7034
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7053..7073
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7055..7073
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7101..7327
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7328..7508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7555..7680
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8173..8406
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8591..8635
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8810..9310
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9229..9247
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9322..9717
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9777..9894
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctc caccatggag tgcagtaaaa tgtccactag gaccccattg
      661 ccaacggtga atgaacgaga cactgaaaac cacacgtcac atggagatgg gcgtcaagaa
      721 gttacctctc gtaccagccg ctcaggagct cggtgtagaa actctatagc ctcctgtgca
      781 gatgaacaac ctcacatcgg aaactacaga ctgttgaaaa caatcggcaa ggggaatttt
      841 gcaaaagtaa aattggcaag acatatcctt acaggcagag aggttgcaat aaaaataatt
      901 gacaaaactc agttgaatcc aacaagtcta caaaagctct tcagagaagt aagaataatg
      961 aagattttaa atcatcccaa tatagtgaag ttattcgaag tcattgaaac tgaaaaaaca
     1021 ctctacctaa tcatggaata tgcaagtgga ggtgaagtat ttgactattt ggttgcacat
     1081 ggcaggatga aggaaaaaga agcaagatct aaatttagac agattgtgtc tgcagttcaa
     1141 tactgccatc agaaacggat cgtacatcga gacctcaagg ctgaaaatct attgttagat
     1201 gccgatatga acattaaaat agcagatttc ggttttagca atgaatttac tgttggcggt
     1261 aaactcgaca cgttttgtgg cagtcctcca tacgcagcac ctgagctctt ccagggcaag
     1321 aaatatgacg ggccagaagt ggatgtgtgg agtctggggg tcattttata cacactagtc
     1381 agtggctcac ttccctttga tgggcaaaac ctaaaggaac tgagagagag agtattaaga
     1441 gggaaataca gaattccctt ctacatgtct acagactgtg aaaaccttct caaacgtttc
     1501 ctggtgctaa atccaattaa acgcggcact ctagagcaaa tcatgaagga caggtggatc
     1561 aatgcagggc atgaagaaga tgaactcaaa ccatttgttg aaccagagct agacatctca
     1621 gaccaaaaaa gaatagatat tatggtggga atgggatatt cacaagaaga aattcaagaa
     1681 tctcttagta agatgaaata cgatgaaatc acagctacat atttgttatt ggggagaaaa
     1741 tcttcagagc tggatgctag tgattccagt tctagcagca atctttcact tgctaaggtt
     1801 aggccgagca gtgatctcaa caacagtact ggccagtctc ctcaccacaa agtgcagaga
     1861 agtgtttctt caagccaaaa gcaaagacgc tacagtgacc atgctggacc agctattcct
     1921 tctgttgtgg cgtatccgaa aaggagtcag accagcactg cagatagtga cctcaaagaa
     1981 gatggaattt cctcccggaa atcaagtggc agtgctgttg gaggaaaggg aattgctcca
     2041 gccagtccca tgcttgggaa tgcaagtaat cctaataagg cggatattcc tgaacgcaag
     2101 aaaagctcca ctgtccctag tagtaacaca gcatctggtg gaatgacacg acgaaatact
     2161 tatgtttgca gtgagagaac tacagctgat agacactcag tgattcagaa tggcaaagaa
     2221 aacagcacta ttcctgatca gagaactcca gttgcttcaa cacacagtat cagtagtgca
     2281 gccaccccag atcgaatccg cttcccaaga ggcactgcca gtcgtagcac tttccacggc
     2341 cagccccggg aacggcgaac cgcaacatat aatggccctc ctgcctctcc cagcctgtcc
     2401 catgaagcca caccattgtc ccagactcga agccgaggct ccactaatct ctttagtaaa
     2461 ttaacttcaa aactcacaag gagtcgcaat gtatctgctg agcaaaaaga tgaaaacaaa
     2521 gaagcaaagc ctcgatccct acgcttcacc tggagcatga aaaccactag ttcaatggat
     2581 cccggggaca tgatgcggga aatccgcaaa gtgttggacg ccaataactg cgactatgag
     2641 cagagggagc gcttcttgct cttctgcgtc cacggagatg ggcacgcgga gaacctcgtg
     2701 cagtgggaaa tggaagtgtg caagctgcca agactgtctc tgaacggggt ccggtttaag
     2761 cggatatcgg ggacatccat agccttcaaa aatattgctt ccaaaattgc caatgagcta
     2821 aagctgtaac ccagtgatta tgaacccagc tttcttgtac aaagtggttg gtaagcctat
     2881 ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg
     2941 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3001 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3061 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3121 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3181 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3241 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3301 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3361 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3421 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3481 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3541 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3601 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     3661 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3841 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     3901 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     3961 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4021 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4081 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4141 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4201 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     4261 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4321 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4381 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4441 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4501 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4561 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4621 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4681 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4741 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4801 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4861 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4921 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4981 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5041 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5101 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5161 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5221 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5281 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5341 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5401 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5461 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5521 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5581 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5641 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5701 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5761 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5821 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5881 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5941 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6901 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6961 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7021 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     7081 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7141 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7201 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7261 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     7321 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7381 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7441 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7501 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7561 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7621 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7681 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7741 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7801 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7861 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7921 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7981 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8041 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8101 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8161 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8221 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8281 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8341 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8401 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8461 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8521 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8581 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     8641 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     8701 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     8761 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct tgcgcctttt
     8821 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     8881 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     8941 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     9001 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     9061 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     9121 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     9181 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     9241 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     9301 ctctccccag ggggtaccac catggccaag cctttgtctc aagaagaatc caccctcatt
     9361 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     9421 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     9481 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     9541 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     9601 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     9661 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg
     9721 cagctgcagt agtaagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
     9781 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     9841 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     9901 tattacaggg acagcagaga tccactttgg cgccggctcg aggggg
//
