LOCUS       sequence-20227-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKgfplux2
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..91)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
       61 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      121 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      181 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      241 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      301 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      361 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      421 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      481 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      541 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
      601 gctgttagcg ggcccattta gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
      661 atatct
//
