LOCUS       sequence-202270-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector for expression in mammalian cells encoding MEF2C
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pRecLV105 MEF2C
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    93..210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        259..769
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     688..706
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             791..1390
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(791..810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1287..1307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1529..1762
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1834..1955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1871..1890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1925..1944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1995..2130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2150..2169)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2151..2169)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2179..2196)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2179..2195)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2210)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2337..2792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2424..2443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2634..2655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2818..2922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2923..2991,2992..3783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3141..3160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3954..4542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4443..4462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4909..4929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4944..4949,4950..4967,4968..4974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4982..4998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4987..5009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5006..5022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5006..5022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5041..5061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5043..5061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5089..5315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5316..5496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5543..5668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6161..6394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6579..6623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6921..7169
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6921..6941
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7092..7111
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        7325..7704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7705..7908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7859..7879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7905..7929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7987..8011
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(9461..9467,1..18))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttcttgtac aaagtggttg atcgcgtgca tgcgacgtca tagctctctc ccaattctcg
       61 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      121 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      181 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      241 gtttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
      301 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
      361 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
      421 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
      481 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
      541 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
      601 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
      661 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
      721 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc
      781 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
      841 cagggcggta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
      901 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
      961 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     1021 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     1081 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     1141 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     1201 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     1261 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     1321 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     1381 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     1441 tgcatcgtta agagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     1501 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     1561 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1621 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1681 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1741 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     1801 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     1861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1921 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     1981 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2041 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2101 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     2161 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2221 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2281 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     2341 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2401 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2461 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2521 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2581 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2641 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2701 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2761 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     2821 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2881 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2941 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3001 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3061 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3121 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3181 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3241 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3301 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3361 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3421 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3481 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3541 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3601 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3661 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3721 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3781 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3841 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3901 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3961 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4021 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4081 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4561 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4621 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4681 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc tttgagtgag
     4741 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4801 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaaaa tgtgagttag
     4921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     5041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     5101 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     5161 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     5221 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     5281 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     5341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5521 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     5581 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     5641 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     5701 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     5761 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     5821 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     5881 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     5941 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6001 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6061 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6121 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6181 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6241 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6301 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     6361 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     6421 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     6481 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     6541 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     6601 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     6661 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     6721 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     6781 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     6841 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     6901 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     6961 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     7021 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     7081 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     7141 tatatatctt gtggaaagga cgaaacaccg gtgatctata cattgaatca atattggcaa
     7201 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     7261 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     7321 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7381 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7441 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7501 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7561 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     7621 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     7681 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     7741 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     7801 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     7861 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     7921 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagatt
     7981 cgaagaacaa gtttgtacaa aaaagcagaa gggccgtcaa ggcccaccat ggggagaaaa
     8041 aagattcaga ttacgaggat tatggatgaa cgtaacagac aggtgacatt tacaaagagg
     8101 aaatttgggt tgatgaagaa ggcttatgag ctgagcgtgc tgtgtgactg tgagattgcg
     8161 ctgatcatct tcaacagcac caacaagctg ttccagtatg ccagcaccga catggacaaa
     8221 gtgcttctca agtacacgga gtacaacgag ccgcatgaga gccggacaaa ctcagacatc
     8281 gtggagacgt tgagaaagaa gggccttaat ggctgtgaca gcccagaccc cgatgcggac
     8341 gattccgtag gtcacagccc tgagtctgag gacaagtaca ggaaaattaa cgaagatatt
     8401 gatctaatga tcagcaggca aagattgtgt gctgttccac ctcccaactt cgagatgcca
     8461 gtctccatcc cagtgtccag ccacaacagt ttggtgtaca gcaaccctgt cagctcactg
     8521 ggaaacccca acctattgcc actggctcac ccttctctgc agaggaatag tatgtctcct
     8581 ggtgtaacac atcgacctcc aagtgcaggt aacacaggtg gtctgatggg tggagacctc
     8641 acgtctggtg caggcaccag tgcagggaac gggtatggca atccccgaaa ctcaccaggt
     8701 ctgctggtct cacctggtaa cttgaacaag aatatgcaag caaaatctcc tcccccaatg
     8761 aatttaggaa tgaataaccg taaaccagat ctccgagttc ttattccacc aggcagcaag
     8821 aatacgatgc catcagtgtc tgaggatgtc gacctgcttt tgaatcaaag gataaataac
     8881 tcccagtcgg ctcagtcatt ggctacccca gtggtttccg tagcaactcc tactttacca
     8941 ggacaaggaa tgggaggata tccatcagcc atttcaacaa catatggtac cgagtactct
     9001 ctgagtagtg cagacctgtc atctctgtct gggtttaaca ccgccagcgc tcttcacctt
     9061 ggttcagtaa ctggctggca acagcaacac ctacataaca tgccaccatc tgccctcagt
     9121 cagttgggag cttgcactag cactcattta tctcagagtt caaatctctc cctgccttct
     9181 actcaaagcc tcaacatcaa gtcagaacct gtttctcctc ctagagaccg taccaccacc
     9241 ccttcgagat acccacaaca cacgcgccac gaggcgggga gatctcctgt tgacagcttg
     9301 agcagctgta gcagttcgta cgacgggagc gaccgagagg atcaccggaa cgaattccac
     9361 tcccccattg gactcaccag accttcgccg gacgaaaggg aaagtccctc agtcaagcgc
     9421 atgcgacttt ctgaaggatg ggcaacataa ggcctcatgg gcccagc
//
