LOCUS       sequence-202305-       11713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous, cobalt-inducible expression of SQE1 from A. thaliana 
            in Synechocystis sp. PCC6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Loeschcke A, Dienst D, Wewer V, Hage-Hulsmann J, Dietsch M, 
            Kranz-Finger S, Huren V, Metzger S, Urlacher VB, Gigolashvili T, 
            Kopriva S, Axmann IM, Drepper T, Jaeger KE
  TITLE     The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis
            sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis.
  JOURNAL   PLoS One. 2017 Dec 27;12(12):e0189816. doi: 
            10.1371/journal.pone.0189816. eCollection 2017.
  PUBMED    29281679
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-12-27"; volume: "12(12):e0189816. doi"; pages: " 
            10.1371/journal.pone.0189816. eCollection 2017"
COMMENT     Sequence Label: pVZ_PcoaT_SQE1
FEATURES             Location/Qualifiers
     source          1..11713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(428..1243)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(428..1243)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1151..1170
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1235..1254
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2101..2495
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            2837..2924
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4163..5134
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5645..6484
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6471..7322
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     misc_feature    7529..7550
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(10357..10375)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10371..10418
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10508..11299)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctagaggatc tgtaatccgg gcagcgcaac ggaacattca tcagtgtaaa aatggaatca
       61 ataaagccct gcgcagcgcg cagggtcagc ctgaatacgc gtttaatgac cagcacagtc
      121 gtgatggcaa ggtcagaata gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca
      181 taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga
      241 gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct
      301 gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa
      361 caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca
      421 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
      481 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
      541 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
      601 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
      661 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
      721 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
      781 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
      841 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
      901 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
      961 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     1021 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     1081 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     1141 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     1201 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     1261 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     1321 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     1381 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     1441 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
     1501 tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag
     1561 gcagacctca gcgctattct gaccttgcca tcacgactgt gctggtcatt aaacgcgtat
     1621 tcaggctgac cctgcgcgct gcgcagggct ttattgattc catttttaca ctgatgaatg
     1681 ttccgttgcg ctgcccggat tacagctgaa agcgaccagg tgctcggcgt ggcaagactc
     1741 gcagcgaacc cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc
     1801 ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg
     1861 gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg
     1921 accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct
     1981 tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg
     2041 aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca
     2101 cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa
     2161 ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa
     2221 atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg
     2281 cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg
     2341 ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa
     2401 accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt
     2461 ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca
     2521 ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc
     2581 gttcaaggta cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca
     2641 ccttggccaa aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt
     2701 gctgctgttc ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt
     2761 cgagcttggc cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt
     2821 gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact
     2881 ttgtagggga gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt
     2941 cgggcggtgc gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg
     3001 cagcatggcg atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc
     3061 cagggccaag gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt
     3121 cttgcacgcc gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga
     3181 tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct
     3241 gccggtcgag ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct
     3301 gaccggtgcc gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc
     3361 gcactgccac ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca
     3421 gtggttcaag cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaccgaagc
     3481 gctcaagccc aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg
     3541 ggcattagag cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg
     3601 catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg
     3661 catccgcacc gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat
     3721 cgacttacag gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat
     3781 ccagaggcat caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac
     3841 tggccgacga agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg
     3901 cggagtggct gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt
     3961 tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg
     4021 ggtggcactg gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc
     4081 tgctgatcgc atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga
     4141 tctggctgcg cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca
     4201 gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca
     4261 gatgatgaac cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg
     4321 gatgaatgcc cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct
     4381 ggtgctggtg gacgacctca gcgagtttga cctggatgac atgaaagccg agggccggga
     4441 gcctgccctg gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga
     4501 cgccgcaggc ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc
     4561 cgacccggcc agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa
     4621 ggacaagcac accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg
     4681 caagaccgcc accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc
     4741 ccagcggcag caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct
     4801 tagccgccac cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa
     4861 gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg
     4921 gggccgcagt gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga
     4981 gcgcaagccc ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct
     5041 gcccagcgtc cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg
     5101 catggacagg ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt
     5161 atactatgag tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg
     5221 gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac
     5281 gtcaaacaag gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga
     5341 cggctgcacc ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg
     5401 tgactgatat gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg
     5461 ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt
     5521 tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg
     5581 cgcagggcgg gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca
     5641 atcaatggct acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc
     5701 gctggactac gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg
     5761 tggtgccggt aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga
     5821 tctgctggag gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc
     5881 gcccaccgcc attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg
     5941 gcaagccgtg gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat
     6001 ggccccggag tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct
     6061 ggacacgctg cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt
     6121 catcggtcgc atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca
     6181 tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag
     6241 ctcggtactg gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga
     6301 ggccgaggaa tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag
     6361 caaggccaac tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt
     6421 gctcaagccc gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc
     6481 ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg
     6541 gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg
     6601 actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc
     6661 tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg
     6721 aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg
     6781 tcaccgctga tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg
     6841 cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga
     6901 aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt
     6961 acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg
     7021 ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca
     7081 gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg
     7141 gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga
     7201 ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct
     7261 ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct
     7321 gacccccccc actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt
     7381 tcctgctaat tggtaatacc atgaaaaata ccatgctcag aaaaggctta acaatatttt
     7441 gaaaaattgc ctactgagcg ctgccgcaca gctccatagg ccgctttcct ggctttgctt
     7501 ccagatgtat gctcttctgc tcctgcatga attcgcggcc gcttctagag actaaagaca
     7561 agtgagatag cagtggcaat ctggctttgc aatcaatgtt tccactaaag cgtttagcgt
     7621 tactgcggct agaagtcctc caccgaggct cccctgaatg gtgatatggg gaatgggact
     7681 ggtcatcagt cgtcgttttg cccccggagc atgactaaaa ccgatcggca ttccgatcac
     7741 aagagccggc tgaatatgtt gttgctctat cagcttacag gcagtgagta aaacagaagg
     7801 ggcatagccg atcgccagca cacatccttg gggaatctgt tgtaaccgct gttgccaatg
     7861 gtcatggtgc caaaaagctt gctcggcttc cctaagccct gtgatgtgag ggtcgtcaat
     7921 cagcgtttta accgtacatc ctaaatgagc taaccgagtt tgatcaagag ccgcagccac
     7981 aaccggaaca tcggtgacga ctggacaccc tgctttcagt gcatctcgtg ccgaggcgat
     8041 cgctccctga ctcaatcgaa cggcgtttac caagctaaca tcaccacagg ccagcactaa
     8101 ttgatgtagt aagtgaatgg taatttcaga gtaagccgat aaatccggta gcaggtgttt
     8161 gagggattcc tgaaaggctt ctggatgagt tgttgtctcc gcatctaggt tcgtccacaa
     8221 ctgatcgagt tttcctaacc cctcctggac atccacatca agctgtttca gttgggccag
     8281 agcttccgct tgggtaatct ggcaactctg gtcgcgtccc agtaatcctt ctaaagcaga
     8341 tgcggtttgg cggagtcgag taatctgctg aatcacagcc tgatattgct gttgcaactg
     8401 caccattagg gtgggatcaa ggctctcttc agaatggcta tccagcagtt gccgaatatg
     8461 agacaactga aagccctgct gtttgagggc aatgactcgt tggagccgtt gtacgtcctg
     8521 ctgagtataa aggcggtagt tgccctctga gcgttgaacg gggggaagca atcccagggt
     8581 gtggtaatgg cgcaccatgc gaggcgtaac gccacctccc actgcatctg tgagttcttt
     8641 aatcgttaag tgattagtct tcatcccttt agtttactca aaaccttgac attgacacta
     8701 atgttaaggt ttaggctgag aaggtaaaaa tccaagttaa aaagcatggc tagccagctg
     8761 tggaactgga tcctgccgct gctgatcagc agcctgctga tcagcttcgt ggcgttctat
     8821 ggcttcttcg tgaaaccgaa acgcaacggc ctgcgccatg atcgcaaaac cgtgagcacc
     8881 gtgaccagcg atgtgggcag cgtgaacatc accggcgata ccgtggcgga tgtgatcgtg
     8941 gtgggcgcgg gcgtggcggg cagcgcgctg gcgtataccc tgggcaaaga taaacgccgc
     9001 gtgcatgtga tcgaacgcga tctgagcgaa ccggatcgca tcgtgggcga actgctgcag
     9061 ccgggcggct atctgaaact gctggaactg ggcatcgaag attgcgtgga agaaatcgat
     9121 gcgcagcgcg tgtatggcta tgcgctgttc aaaaacggca aacgcatccg cctggcgtat
     9181 ccgctggaaa aattccatga agatgtgagc ggccgcagct tccataacgg ccgcttcatc
     9241 cagcgcatgc gcgaaaaagc ggcgagcctg ccgaacgtgc agctggaaca gggcaccgtg
     9301 ctgagcctgc tggaagaaaa cggcaccatc aaaggcgtgc gctataaaaa caaagcgggc
     9361 gaagaacaga ccgcgttcgc ggcgctgacc atcgtgtgcg atggctgctt cagcaacctg
     9421 cgccgcagcc tgtgcaaccc gcaggtggaa gtgccgagct gcttcgtggg cctggtgctg
     9481 gaaaactgca acctgccgta tgcgaaccat ggccatgtgg tgctggcgga tccgagcccg
     9541 atcctgatgt atccgatcag cagcaccgaa gtgcgctgcc tggtggatgt gccgggccag
     9601 aaagtgccga gcatcgcgaa cggcgaaatg aaaaactatc tgaaaaccgt ggtggcgccg
     9661 cagatgccgc atgaagtgta tgatagcttc atcgcggcgg tggataaagg caacatcaaa
     9721 agcatgccga accgcagcat gccggcgagc ccgtatccga ccccgggcgc gctgctgatg
     9781 ggcgatgcgt tcaacatgcg ccatccgctg accggcggcg gcatgaccgt ggcgctggcg
     9841 gatatcgtgg tgctgcgcaa cctgctgcgc ccgctgcgcg atctgagcga tggcgcgagc
     9901 ctgtgcaaat atctggagag cttctatacc ctgcgcaaac cggtggcggc gaccatcaac
     9961 accctggcga acgcgctgta tcaggtgttc tgcagcagcg aaaacgaagc gcgcaacgaa
    10021 atgcgcgaag cgtgcttcga ttatctgggc ctgggcggca tgtgcaccag cggcccggtg
    10081 agcctgctga gcggcctgaa cccgcgcccg ctgaccctgg tgtgccattt cttcgcggtg
    10141 gcggtgtatg gcgtgatccg cctgctgatc ccgttcccga gcccgaaacg catctggctg
    10201 ggcgcgaaac tgatcagcgg cgcgagcggc atcatcttcc cgatcatcaa agcggaaggc
    10261 gtgcgccaga tgttcttccc ggcgaccgtg ccggcgtatt attataaagc gccgaccgtg
    10321 ggcgaaacca aatgcagcta acctaggctg ctgccaccgc tgagcaataa ctagcataac
    10381 cccttggggc ctctaaacgg gtcttgaggg gttttttggg cgactagtag cggccgctgc
    10441 agtcgactct agagcttgag ttaagctgcg ccgcgaagcg gcgtcggctt gaacgaattg
    10501 ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
    10561 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
    10621 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
    10681 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
    10741 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
    10801 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
    10861 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
    10921 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
    10981 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcataaca atggtgactt
    11041 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
    11101 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
    11161 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
    11221 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
    11281 cgatcaccgc ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
    11341 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
    11401 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
    11461 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
    11521 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
    11581 tccgtttcca cagtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
    11641 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
    11701 ccttgctgtt ctt
//
