LOCUS       sequence-202307-       11205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and 
            Mxi1 domain controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9-Mxi1
FEATURES             Location/Qualifiers
     source          1..11205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..52
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     44..61
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     45..61
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        136..802
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     672..695
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             825..845
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4962..4982
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5016..5138
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      5151..5338
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5206..5230)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(5415..5433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5454..5470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5454..5470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5467..5489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5478..5494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5502..5508,5509..5526,5527..5532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5547..5568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5685..5702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5856..6444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5936..5955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6615..7406,7407..7475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7238..7257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7476..7580)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7617..8120
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8161..8179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8217..8239)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8339..8358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(8852..9946)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9947..10354)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(10654..11109)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10791..10812)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11003..11022
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
       61 tgaattgtaa tacgactcac tatagggcga attggagctc actcggaatt cagtctacta
      121 gtcagttcga gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata
      181 gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc
      241 gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt
      301 gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc
      361 agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag
      421 gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca
      481 acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac
      541 gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa
      601 aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa
      661 gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa
      721 attctacttt tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa
      781 taaacacaca taaacaaaca aaacccgccg ccaccatgga cgtcccaaag aagaagagaa
      841 aggtaggcag cgacaagaag tattctatcg gactggccat cgggactaat agcgtcgggt
      901 gggccgtgat cactgacgag tacaaggtgc cctctaagaa gttcaaggtg ctcgggaaca
      961 ccgaccggca ttccatcaag aaaaatctga tcggagctct cctctttgat tcaggggaga
     1021 ccgctgaagc aacccgcctc aagcggactg ctagacggcg gtacaccagg aggaagaacc
     1081 ggatttgtta ccttcaagag atattctcca acgaaatggc aaaggtcgac gacagcttct
     1141 tccataggct ggaagaatca ttcctcgtgg aagaggataa gaagcatgaa cggcatccca
     1201 tcttcggtaa tatcgtcgac gaggtggcct atcacgagaa atacccaacc atctaccatc
     1261 ttcgcaaaaa gctggtggac tcaaccgaca aggcagacct ccggcttatc tacctggccc
     1321 tggcccacat gatcaagttc agaggccact tcctgatcga gggcgacctc aatcctgaca
     1381 atagcgatgt ggataaactg ttcatccagc tggtgcagac ttacaaccag ctctttgaag
     1441 agaaccccat caatgcaagc ggagtcgatg ccaaggccat tctgtcagcc cggctgtcaa
     1501 agagccgcag acttgagaat cttatcgctc agctgccggg tgaaaagaaa aatggactgt
     1561 tcgggaacct gattgctctt tcacttgggc tgactcccaa tttcaagtct aatttcgacc
     1621 tggcagagga tgccaagctg caactgtcca aggacaccta tgatgacgat ctcgacaacc
     1681 tcctggccca gatcggtgac caatacgccg accttttcct tgctgctaag aatctttctg
     1741 acgccatcct gctgtctgac attctccgcg tgaacactga aatcaccaag gcccctcttt
     1801 cagcttcaat gattaagcgg tatgatgagc accaccagga cctgaccctg cttaaggcac
     1861 tcgtccggca gcagcttccg gagaagtaca aggaaatctt ctttgaccag tcaaagaatg
     1921 gatacgccgg ctacatcgac ggaggtgcct cccaagagga attttataag tttatcaaac
     1981 ctatccttga gaagatggac ggcaccgaag agctcctcgt gaaactgaat cgggaggatc
     2041 tgctgcggaa gcagcgcact ttcgacaatg ggagcattcc ccaccagatc catcttgggg
     2101 agcttcacgc catccttcgg cgccaagagg acttctaccc ctttcttaag gacaacaggg
     2161 agaagattga gaaaattctc actttccgca tcccctacta cgtgggaccc ctcgccagag
     2221 gaaatagccg gtttgcttgg atgaccagaa agtcagaaga aactatcact ccctggaact
     2281 tcgaagaggt ggtggacaag ggagccagcg ctcagtcatt catcgaacgg atgactaact
     2341 tcgataagaa cctccccaat gagaaggtcc tgccgaaaca ttccctgctc tacgagtact
     2401 ttaccgtgta caacgagctg accaaggtga aatatgtcac cgaagggatg aggaagcccg
     2461 cattcctgtc aggcgaacaa aagaaggcaa ttgtggacct tctgttcaag accaatagaa
     2521 aggtgaccgt gaagcagctg aaggaggact atttcaagaa aattgaatgc ttcgactctg
     2581 tggagattag cggggtcgaa gatcggttca acgcaagcct gggtacctac catgatctgc
     2641 ttaagatcat caaggacaag gattttctgg acaatgagga gaaagaggac atccttgagg
     2701 acattgtcct gactctcact ctgttcgagg accgggaaat gatcgaggag aggcttaaga
     2761 cctacgccca tctgttcgac gataaagtga tgaagcaact taaacggaga agatataccg
     2821 gatggggacg ccttagccgc aaactcatca acggaatccg ggacaaacag agcggaaaga
     2881 ccattcttga tttccttaag agcgacggat tcgctaatcg caacttcatg caacttatcc
     2941 atgatgattc cctgaccttt aaggaggaca tccagaaggc ccaagtgtct ggacaaggtg
     3001 actcactgca cgagcatatc gcaaatctgg ctggttcacc cgctattaag aagggtattc
     3061 tccagaccgt gaaagtcgtg gacgagctgg tcaaggtgat gggtcgccat aaaccagaga
     3121 acattgtcat cgagatggcc agggaaaacc agactaccca gaagggacag aagaacagca
     3181 gggagcggat gaaaagaatt gaggaaggga ttaaggagct cgggtcacag atccttaaag
     3241 agcacccggt ggaaaacacc cagcttcaga atgagaagct ctatctgtac taccttcaaa
     3301 atggacgcga tatgtatgtg gaccaagagc ttgatatcaa caggctctca gactacgacg
     3361 tggacgccat cgtccctcag agcttcctca aagacgactc aattgacaat aaggtgctga
     3421 ctcgctcaga caagaaccgg ggaaagtcag ataacgtgcc ctcagaggaa gtcgtgaaaa
     3481 agatgaagaa ctattggcgc cagcttctga acgcaaagct gatcactcag cggaagttcg
     3541 acaatctcac taaggctgag aggggcggac tgagcgaact ggacaaagca ggattcatta
     3601 aacggcaact tgtggagact cggcagatta ctaaacatgt agcccaaatc cttgactcac
     3661 gcatgaatac caagtacgac gaaaacgaca aacttatccg cgaggtgaag gtgattaccc
     3721 tgaagtccaa gctggtcagc gatttcagaa aggactttca attctacaaa gtgcgggaga
     3781 tcaataacta tcatcatgct catgacgcat atctgaatgc cgtggtggga accgccctga
     3841 tcaagaagta cccaaagctg gaaagcgagt tcgtgtacgg agactacaag gtctacgacg
     3901 tgcgcaagat gattgccaaa tctgagcagg agatcggaaa ggccaccgca aagtacttct
     3961 tctacagcaa catcatgaat ttcttcaaga ccgaaatcac ccttgcaaac ggtgagatcc
     4021 ggaagaggcc gctcatcgag actaatgggg agactggcga aatcgtgtgg gacaagggca
     4081 gagatttcgc taccgtgcgc aaagtgcttt ctatgcctca agtgaacatc gtgaagaaaa
     4141 ccgaggtgca aaccggaggc ttttctaagg aatcaatcct ccccaagcgc aactccgaca
     4201 agctcattgc aaggaagaag gattgggacc ctaagaagta cggcggattc gattcaccaa
     4261 ctgtggctta ttctgtcctg gtcgtggcta aggtggaaaa aggaaagtct aagaagctca
     4321 agagcgtgaa ggaactgctg ggtatcacca ttatggagcg cagctccttc gagaagaacc
     4381 caattgactt tctcgaagcc aaaggttaca aggaagtcaa gaaggacctt atcatcaagc
     4441 tcccaaagta tagcctgttc gaactggaga atgggcggaa gcggatgctc gcctccgctg
     4501 gcgaacttca gaagggtaat gagctggctc tcccctccaa gtacgtgaat ttcctctacc
     4561 ttgcaagcca ttacgagaag ctgaagggga gccccgagga caacgagcaa aagcaactgt
     4621 ttgtggagca gcataagcat tatctggacg agatcattga gcagatttcc gagttttcta
     4681 aacgcgtcat tctcgctgat gccaacctcg ataaagtcct tagcgcatac aataagcaca
     4741 gagacaaacc aattcgggag caggctgaga atatcatcca cctgttcacc ctcaccaatc
     4801 ttggtgcccc tgccgcattc aagtacttcg acaccaccat cgaccggaaa cgctatacct
     4861 ccaccaaaga agtgctggac gccaccctca tccaccagag catcaccgga ctttacgaaa
     4921 ctcggattga cctctcacag ctcggagggg atgagggagc tcccaagaaa aagcgcaagg
     4981 taggtagttc caagcttggc ggcagcggcg gcagcatgga acgtgtgaga atgattaatg
     5041 tgcaaaggct gttagaagcc gcagagtttt tagaaagaag agaaagagaa tgcgaacacg
     5101 ggtatgccag ttctttccct agcatgccct ctcccagagg ctaactcgag gcgaatttct
     5161 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     5221 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     5281 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
     5341 tgccgagcaa atgcctgcaa atcgctcccc atttctctag ataagactgc agagttcggt
     5401 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     5461 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5521 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5581 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5761 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc cttttcatca
     7621 cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag
     7681 aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc
     7741 tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat
     7801 gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca
     7861 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata
     7921 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc
     7981 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa
     8041 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg
     8101 taagttacag gcaagcgatc cgtctaagaa accattatta tcatgacatt aacctataaa
     8161 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8221 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     8281 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     8341 gcatcagagc agattgtact gagagtgcac catatcgact acgtcgttaa ggccgtttct
     8401 gacagagtaa aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt
     8461 gacttaaact ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt
     8521 tttttagaga aaatcctcca atatataaat taggaatcat agtttcatga ttttctgtta
     8581 cacctaactt tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct
     8641 ttttccttat cacgttgagc cattagtatc aatttgctta cctgtattcc tttacatcct
     8701 cctttttctc cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg
     8761 aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta
     8821 tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg
     8881 cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac
     8941 ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca
     9001 atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg accttattct
     9061 ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt
     9121 ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg
     9181 cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg
     9241 ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg
     9301 taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca
     9361 aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga
     9421 aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag
     9481 cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc actaattctc
     9541 tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa
     9601 gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga
     9661 tttttagtaa accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag
     9721 cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa
     9781 cacctgtagc atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat
     9841 tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac
     9901 caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc
     9961 cttgaaatat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag
    10021 acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca
    10081 agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt
    10141 ccggcgctct cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca
    10201 ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg
    10261 cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc
    10321 ttgcatctta cgatacctga gtattcccac agttaactgc ggtcaagata tttcttgaat
    10381 caggcgcctt agaccgctcg gccaaacaac caattacttg ttgagaaata gagtataatt
    10441 atcctataaa tataacgttt ttgaacacac atgaacaagg aagtacagga caattgattt
    10501 tgaagagaat gtggattttg atgtaattgt tgggattcca tttttaataa ggcaataata
    10561 ttaggtatgt agatatacta gaagttctcc tcgacggtcg atatgcggtg tgaaataccg
    10621 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
    10681 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    10741 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
    10801 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
    10861 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
    10921 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    10981 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    11041 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    11101 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    11161 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaag
//
