LOCUS       sequence-202311-        5003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin 
            resistance cassette downstream used to replace the native copy of 
            Vipp1 in Synechocystis (sll0617)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH42
FEATURES             Location/Qualifiers
     source          1..5003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2529..3320)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gggcgaattc gagctcggta cccgggtaac gaaaggattc ggcgggtagg gcaaaatctt
       61 gtcagcagac taggaatagc aattaagccc aagcccaact actttaactg ttcaagttgt
      121 ttttccctca tttcactggg ctttttaata tttggctttg gagccatccc caataatttt
      181 tgattaattt gttccctggt gtttttgcta taactagccc agggatcggg caaaaacata
      241 tcaccgatgc tgataaaagc cagaccagca atcaccgctg gaatggcaag ggagcgcaaa
      301 attttaaata gacttgccat aacgctatgc ctccacaatc aaatctcgat tggactacct
      361 gtgtttccat tctagggtag ggcaccatgg cgaaagccac taaaaccaac tccaaggctg
      421 tctagtgtct gcgggcttgg tttacactgg atcaacatag ggccaactcc ttagtccccc
      481 ttatgcggac ttctgtacag acccccattc gttaaccccc agcgattttg agaggataag
      541 taagacatgg gattatttga ccgtttaggc cgcgtcgtcc gggctaacct caacgacctc
      601 gtcagtaaag ctgaagatcc agaaaaagtt ctggaacagg ccgtgatcga tatgcaggaa
      661 gacctggtgc aactccgcca ggccgttgcc cgcacgatcg ccgaagaaaa acgaacagag
      721 caacgtctta accaagacac ccaggaagcc aagaaatggg aagaccgggc aaaattagcc
      781 ctcaccaatg gggaagaaaa tttggctagg gaagccctgg cccgcaaaaa aagtctgaca
      841 gatacggcgg cggcgtacca aacccagcta gcccaacaga ggaccatgtc ggagaatctc
      901 cgtcgcaacc tcgcggctct agaagcaaaa atttccgaag ctaaaaccaa gaaaaatatg
      961 ttgcaggcca gggccaaagc ggccaaggct aatgctgaac tgcagcaaac cctcgggggc
     1021 ttaggtacta gcagtgctac tagtgctttt gaacggatgg agaacaaggt actggatatg
     1081 gaagccactt cccaagcggc aggggagtta gccggctttg gcatcgagaa ccagtttgcc
     1141 cagttggaag ccagcagtgg ggtagaagac gagttggccg ccctgaaagc ttccatggcc
     1201 ggtggagcat taccgggaac ctctgccgct acgccccaac tcgaagcggc tcctgttgat
     1261 tcctcagtac cagctaataa tgccagtcaa gatgatgcgg tgattgacca ggaattggac
     1321 gacctacgtc gtcggttaaa taatctgagt ggtggtggag gtagcaaagg cgaggagctc
     1381 tttactggcg ttgtccccat tctcgtcgaa ctagacgggg atgttaatgg ccataagttc
     1441 tccgtgagtg gcgaagggga aggtgatgcc acgtatggca aactgacgct gaaatttatc
     1501 tgtaccaccg ggaaattgcc ggttccttgg ccaacactcg ttaccacctt tggctatggc
     1561 gtccaatgct ttgcccgtta ccccgatcac atgaagcagc atgacttctt caaaagcgcc
     1621 atgccagagg gttatgtcca agaacgcacg atcttcttca aggacgatgg caactacaag
     1681 acccgagctg aagtgaagtt tgagggagat acgttggtga atcgcatcga actgaaaggg
     1741 atcgacttta aagaggatgg caatattctg gggcataaac tggagtacaa ctacaattcg
     1801 cataacgtgt acattatggc ggataagcag aagaatggca tcaaggtcaa cttcaagatt
     1861 cggcacaata tcgaagatgg ttcagtgcaa ctagcggacc actatcagca gaacacaccg
     1921 attggcgatg gtcctgtcct gttgccggat aaccactatc tgagcactca atcgaaactg
     1981 tctaaggatc ccaacgagaa acgcgatcac atggtgttgc tcgaatttgt gactgctgca
     2041 ggcattaccc atggaatgga cgaactgtac aaataattgg tttaactcag ccagtaaaaa
     2101 tcccccatta acctaggttt tgggggaaat tgatcagatt ggcggatttg gccggggttc
     2161 aggttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct ctagggtccc
     2221 caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac cggatcagct
     2281 tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat tacttcgcca actattgcga
     2341 taacaagaaa aagccagcct ttcatgatat atctcccaat ttgtgtaggg cttattatgc
     2401 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     2461 ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
     2521 gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     2581 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     2641 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     2701 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     2761 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     2821 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     2881 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     2941 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     3001 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     3061 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     3121 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     3181 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     3241 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     3301 gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     3361 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     3421 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     3481 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     3541 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     3601 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     3661 ctgtcctggc tggcgaacga ggattgaatt acaaatgcta gtgggtcaga ctccagcaac
     3721 tatgattagg ggttcgagtt tccctacagg aagcaatcaa agcctcgatc acccacagcc
     3781 agaactgggc aatattacca gctagacttg gttacaccgg gcaaaagacc ctggtgggcc
     3841 cattcccgta aaacattgcg gcaaaggcca aagtcacggt agtaggaacg gggacgacct
     3901 gtaacctgac agcggttacg gcgacggttg ggggcgctat tgcggggtaa atcctgcaat
     3961 ttttggtgaa cagctaattt gtcttctagg gtttcagctt gacggaattc ttctttgagc
     4021 gcttcccgtt tagcagcata ctttgctacc aagcggctac gacgcttatc ccgctcgatc
     4081 atggattttt ttgccatcgg ggtttaactt tatttcttta gtctaaaaat tgagcactcc
     4141 ccatcatatc acactgcttg gggacacaaa ggtttatgaa aggtaatgga actagacgaa
     4201 cacggaggga ttcgaacccc cgaccctcag gaccggaacc tgatgctcta tccactgagc
     4261 tacgtgtcca ggacagactt gggctgaccc tttcatcttg cgaaaagaat tcacaccagc
     4321 tatcagcgta acatttcctg caccgattcg gaagctaact gggcaccatt ttccagtaac
     4381 caagcttcct gactgggagc taaccccgga ttgtcaccaa aactgagatt ttccaccttt
     4441 gcgccactga aattgacgtt ttggagagtg gctccccgca aatccgcttc ccgtaggtct
     4501 gtgccgatta aagttgctag ggctaaacta cttttacgaa aatcaacttg gctgagatta
     4561 gccccttcta ggtaagcgcc actcaggtct gcaccccgaa aattggaccg gcggagatcg
     4621 gtattactca aatcgctgtc ggtcagaata gctccccgga aattgctgtc cattagttgg
     4681 ccattactaa ggtcaatgct gttgaggtta gcacccacaa atttgcctcc cgccaaatcc
     4741 cgttgcgggt ccaaaccgac taacttcccc agggtaaggc aatccaactg gggggagttg
     4801 atgattaatt gcaggcgatc gccaaggttc ccggtgtctg cttccgttac ggtgcgatta
     4861 ttttcgacat tgtatagagg ttgccagata atttgaggat cggcatcgac tgatttgctc
     4921 aggagccatt gaccccaaca aagggccgga gttaggtaat tttccgctat aaacttacat
     4981 gcaagcttga gtattctata gtg
//
