LOCUS       sequence-202312-        5336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor
            away Vipp1 (sll0617) of Synecocystis PCC6803..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH50
FEATURES             Location/Qualifiers
     source          1..5336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2083..2409
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLET"
     CDS             complement(2862..3653)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gggcgaattc gagctcggta cccgggtaac gaaaggattc ggcgggtagg gcaaaatctt
       61 gtcagcagac taggaatagc aattaagccc aagcccaact actttaactg ttcaagttgt
      121 ttttccctca tttcactggg ctttttaata tttggctttg gagccatccc caataatttt
      181 tgattaattt gttccctggt gtttttgcta taactagccc agggatcggg caaaaacata
      241 tcaccgatgc tgataaaagc cagaccagca atcaccgctg gaatggcaag ggagcgcaaa
      301 attttaaata gacttgccat aacgctatgc ctccacaatc aaatctcgat tggactacct
      361 gtgtttccat tctagggtag ggcaccatgg cgaaagccac taaaaccaac tccaaggctg
      421 tctagtgtct gcgggcttgg tttacactgg atcaacatag ggccaactcc ttagtccccc
      481 ttatgcggac ttctgtacag acccccattc gttaaccccc agcgattttg agaggataag
      541 taagacatgg gattatttga ccgtttaggc cgcgtcgtcc gggctaacct caacgacctc
      601 gtcagtaaag ctgaagatcc agaaaaagtt ctggaacagg ccgtgatcga tatgcaggaa
      661 gacctggtgc aactccgcca ggccgttgcc cgcacgatcg ccgaagaaaa acgaacagag
      721 caacgtctta accaagacac ccaggaagcc aagaaatggg aagaccgggc aaaattagcc
      781 ctcaccaatg gggaagaaaa tttggctagg gaagccctgg cccgcaaaaa aagtctgaca
      841 gatacggcgg cggcgtacca aacccagcta gcccaacaga ggaccatgtc ggagaatctc
      901 cgtcgcaacc tcgcggctct agaagcaaaa atttccgaag ctaaaaccaa gaaaaatatg
      961 ttgcaggcca gggccaaagc ggccaaggct aatgctgaac tgcagcaaac cctcgggggc
     1021 ttaggtacta gcagtgctac tagtgctttt gaacggatgg agaacaaggt actggatatg
     1081 gaagccactt cccaagcggc aggggagtta gccggctttg gcatcgagaa ccagtttgcc
     1141 cagttggaag ccagcagtgg ggtagaagac gagttggccg ccctgaaagc ttccatggcc
     1201 ggtggagcat taccgggaac ctctgccgct acgccccaac tcgaagcggc tcctgttgat
     1261 tcctcagtac cagctaataa tgccagtcaa gatgatgcgg tgattgacca ggaattggac
     1321 gacctacgtc gtcggttaaa taatctgagt ggtggtggag gtagcaaagg cgaggagctc
     1381 tttactggcg ttgtccccat tctcgtcgaa ctagacgggg atgttaatgg ccataagttc
     1441 tccgtgagtg gcgaagggga aggtgatgcc acgtatggca aactgacgct gaaatttatc
     1501 tgtaccaccg ggaaattgcc ggttccttgg ccaacactcg ttaccacctt tggctatggc
     1561 gtccaatgct ttgcccgtta ccccgatcac atgaagcagc atgacttctt caaaagcgcc
     1621 atgccagagg gttatgtcca agaacgcacg atcttcttca aggacgatgg caactacaag
     1681 acccgagctg aagtgaagtt tgagggagat acgttggtga atcgcatcga actgaaaggg
     1741 atcgacttta aagaggatgg caatattctg gggcataaac tggagtacaa ctacaattcg
     1801 cataacgtgt acattatggc ggataagcag aagaatggca tcaaggtcaa cttcaagatt
     1861 cggcacaata tcgaagatgg ttcagtgcaa ctagcggacc actatcagca gaacacaccg
     1921 attggcgatg gtcctgtcct gttgccggat aaccactatc tgagcactca atcgaaactg
     1981 tctaaggatc ccaacgagaa acgcgatcac atggtgttgc tcgaatttgt gactgctgca
     2041 ggcattaccc atggaatgga cgaactgtac aaaggaagtg gtggcgttca agtggagaca
     2101 atttctcctg gcgacggtcg tacctttccc aaacggggac aaacgtgcgt ggtccattat
     2161 accggtatgc tggaagatgg caaaaaattt gatagcagcc gtgatcgtaa taaacccttt
     2221 aaatttatgc tagggaaaca agaggtaatt cgggggtggg aagagggagt ggcccaaatg
     2281 tctgtcgggc agcgagctaa attgactatc tctcctgatt acgcatatgg cgcgactggg
     2341 catcccggca tcattccccc tcatgccacc ctagtctttg atgttgaatt gttaaaatta
     2401 gaaacataat tggtttaact cagccagtaa aaatccccca ttaacctagg ttttggggga
     2461 aattgatcag attggcggat ttggccgggg ttcaggttgt aaaccgtttt gtgaaaaaat
     2521 ttttaaaata aaaaagggga cctctagggt ccccaattaa ttagtaatat aatctattaa
     2581 aggtcattca aaaggtcatc caccggatca gcttagtaaa gccctcgcta gattttaatg
     2641 cggatgttgc gattacttcg ccaactattg cgataacaag aaaaagccag cctttcatga
     2701 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt
     2761 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaacgct tgagttaagc
     2821 cgcgccgcga agcggcgtcg gcttgaacga attgttagac attatttgcc gactaccttg
     2881 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     2941 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     3001 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     3061 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     3121 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     3181 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     3241 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     3301 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     3361 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     3421 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     3481 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     3541 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     3601 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt
     3661 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     3721 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     3781 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     3841 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     3901 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     3961 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgaggattga
     4021 attacaaatg ctagtgggtc agactccagc aactatgatt aggggttcga gtttccctac
     4081 aggaagcaat caaagcctcg atcacccaca gccagaactg ggcaatatta ccagctagac
     4141 ttggttacac cgggcaaaag accctggtgg gcccattccc gtaaaacatt gcggcaaagg
     4201 ccaaagtcac ggtagtagga acggggacga cctgtaacct gacagcggtt acggcgacgg
     4261 ttgggggcgc tattgcgggg taaatcctgc aatttttggt gaacagctaa tttgtcttct
     4321 agggtttcag cttgacggaa ttcttctttg agcgcttccc gtttagcagc atactttgct
     4381 accaagcggc tacgacgctt atcccgctcg atcatggatt tttttgccat cggggtttaa
     4441 ctttatttct ttagtctaaa aattgagcac tccccatcat atcacactgc ttggggacac
     4501 aaaggtttat gaaaggtaat ggaactagac gaacacggag ggattcgaac ccccgaccct
     4561 caggaccgga acctgatgct ctatccactg agctacgtgt ccaggacaga cttgggctga
     4621 ccctttcatc ttgcgaaaag aattcacacc agctatcagc gtaacatttc ctgcaccgat
     4681 tcggaagcta actgggcacc attttccagt aaccaagctt cctgactggg agctaacccc
     4741 ggattgtcac caaaactgag attttccacc tttgcgccac tgaaattgac gttttggaga
     4801 gtggctcccc gcaaatccgc ttcccgtagg tctgtgccga ttaaagttgc tagggctaaa
     4861 ctacttttac gaaaatcaac ttggctgaga ttagcccctt ctaggtaagc gccactcagg
     4921 tctgcacccc gaaaattgga ccggcggaga tcggtattac tcaaatcgct gtcggtcaga
     4981 atagctcccc ggaaattgct gtccattagt tggccattac taaggtcaat gctgttgagg
     5041 ttagcaccca caaatttgcc tcccgccaaa tcccgttgcg ggtccaaacc gactaacttc
     5101 cccagggtaa ggcaatccaa ctggggggag ttgatgatta attgcaggcg atcgccaagg
     5161 ttcccggtgt ctgcttccgt tacggtgcga ttattttcga cattgtatag aggttgccag
     5221 ataatttgag gatcggcatc gactgatttg ctcaggagcc attgacccca acaaagggcc
     5281 ggagttaggt aattttccgc tataaactta catgcaagct tgagtattct atagtg
//
