LOCUS       sequence-202313-        4324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HU-FRB construct (anchor) expressed in trans from the slr0168 
            neutral site of Synechocystis PCC6803. Used to anchor away any FKBP 
            tagged target in presence of rapamycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH51
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1349..2164)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1349..2164)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2072..2091
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2156..2175
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gccggtactg ccgggcctct tgcgggattc ggcgataatc tcccctgggt cggtttttga
       61 ctggggaaag aagagaaaca cggcctgtgc tagtatctaa ggcatgaatt ttcgtgaagt
      121 taattataac agtcagtatt ttgcggctgg ctaggtgttt aggagtggca acagcatcat
      181 gacgagccat cagctcaacg ggcaacggag ttaccttcag ccgatccgca tcggggtgat
      241 tggggtgggc aatatgggac agcaccatac cagagtcctg agcctgatga aggatgtgga
      301 gtttgtgggt attgccgatg tcaatgtgga acggggccta gacaccgcca gtaagtatcg
      361 ggtgcatttt tttgaagatt atcaggagat gttgccccat gtggatgcgg tttgtgtggc
      421 ggttcccact aggctccatc acgatgtggg catgaattgt ttgcaaaata atgtccatac
      481 tctgattgaa aaacccattg ccgctagcat tgctgaagcg gaatccctgg ttaatgccgc
      541 cgccgatgcc aattgcattc tccaagtggg gcacattgaa cgcttcaacc cggcattttt
      601 agagctaacc aaaattctca aaacggaaga gttattggcg atcgaagccc atcgcatgag
      661 tccctattcc cagcgggcca atgatgtctc cgtggtattg gatttgatga tccatgacat
      721 tgacctgttg ctggaattgg tgggttcgga agtggttaaa ctgtccgcca gtggcagtcg
      781 ggcttctggg tcaggatatt tggattatgt caccgctacg ttaggcttct cctccggcat
      841 tgtggccacc ctcaccgcca gtaaggtcac ccatcgtaaa attcgttcca tcgccgccca
      901 ctgcaaaaat tccctcaccg aagcggattt tctcaataac gaaattttga tccatcgcca
      961 aaccaccgct gattggagcg cggactatgg ccaggtattg tatcgccagg atggtctaat
     1021 cgaaaaggtt tacaccagta atattgaacc tctccacgct gaattagaac attttattca
     1081 ttgtgttagg ggaggtgatc aaccctcagt ggggggagaa caggccctca aggccctgaa
     1141 gttagccagt ttaattgaag aaatggccct ggacagtcag gaatggcatg ggggggaagt
     1201 tgtgacagaa tatcaagatg ccaccctggc cctcagtgcg agtgtttaaa tcaacttaat
     1261 taatgcaatt attgcgagtt caaactcatt tctgtcctgg ctggcgaacg agccgctgcc
     1321 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1381 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1441 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1501 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1561 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     1621 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     1681 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     1741 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     1801 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     1861 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     1921 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     1981 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     2041 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2101 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     2161 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     2221 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttgttgaa
     2281 taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac agggtaaaat
     2341 ccaagttttc gtaaaggatt gtgacaaaat cccgcaaaac tgcaaaaatt cgtggggaaa
     2401 agtgatccta cttccacact ttgtgcatag aataaggcag tgaaacagcc agtctgcggg
     2461 gatttcaggg gtttttaatg ccaaatttct gtgtctatcc gggctgtgga gcctcccgat
     2521 gaccctgtta tggggagaaa atgcctaaaa aatgagtcta ttccaacaaa ctcactctca
     2581 aggagatttt ttcagatgaa taaaggcgaa ttagtccatg cggtcacgga caaagcgaaa
     2641 gagcaactgg gggaaagcat cactaaaaag gaggtagatg cggtaatttc agccgccatt
     2701 gattgcatca tggaagtggt ggccgaaggg gaaaaagtaa ccctagtggg ctttggttcc
     2761 tttgaagcca gggaacgtaa ggaacgggag ggccgtaacc ccaaaactgg ggataaaatg
     2821 ctcattccag ccactaaggt gccggctttc tccgcaggca aaatgttcaa agataaagtt
     2881 gctcctgaga aaaaaggaag tggtatgtgg catgaagggc tagaagaggc atctcgtcta
     2941 tattttggcg agcggaatgt taaaggcatg tttgaagttt tggaacctct gcatgctatg
     3001 atggaacgcg gtccccaaac attaaaggaa accagtttta accaagcata tgggcgtgat
     3061 ttaatggaag cccaggaatg gtgtcgcaag tatatgaagt ctggtaatgt taaggatctg
     3121 ttgcaagcct gggatctata ctatcatgtg ttccgccgta ttagtaagta attggtttaa
     3181 ctcagccagt aaaaatcccc cattaaccta ggttttgggg gaaattgatg ggatttggcc
     3241 ggggttcagg acagctatga cctgacttac atcgaagatc atgacaacta ttacgacatt
     3301 atcctcaaag gggacgaagc cgcagttcgc caaattaaga gggttgcttt gccctccgaa
     3361 ggggattatt cggcggttta taatcccggt ggccccggca atgatccaga gaatggtccc
     3421 ccagggccct ttactgtgtc cagtagtccc caggtaatta aggtaacgga taccatcggc
     3481 cagcccacca aagtctccta tgtggaagtg gatggccccg tattgcgtaa tcccttcagt
     3541 ggtactccca ttgggcaaga ggtgggttta gcggttaaag atctggccac aggtcatgaa
     3601 atttatcagt acactgaccc agatgggaag gtattttatg cttcctttgc tgccgctgat
     3661 gaccaagcca cggatttaac cacggcgatc gccaatccca cggccatcga tttaattaac
     3721 gccaggggat ttacggcggg tagttccgtc accgtatcgg gttcctacag tcgggaagcc
     3781 ttttttgatg gatccatggg tttttatcga cttctggacg ataacggtgc agtgctagat
     3841 cccttaacag gtggtgtaat caacccagga caggtaggtt atcaagaagc agctttggca
     3901 gatagcaatc gtttgcaagc cactggctcc accctaacgg cagaagacct agaaaccaga
     3961 gcattttcct tcaatatttt gggtggcgag ttgtatgcgc catttttaac ggttaatgac
     4021 agtctttccg gtattaatca gacttatttt gcctttgggt cggccaaccc agatggcatc
     4081 agccacagca caaacttggg acccaacgtg attggttttg aagattttct cggcggagga
     4141 gacctagatt tcgacgacat cattgtcaga tttacgctga cttagctcag gggcattatc
     4201 ggagcaagcc gatattcagg taagatgggg cggtgctttt gtgggttgat ccattagctt
     4261 taaccccatt gtctccactg acggtaatag ttttcccgct atatgcgttg atgcaatttc
     4321 tatg
//
