LOCUS       sequence-202315-        4716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains CurT-mTurquoise2 construct used to replace the native CurT 
            in Synechocystis PCC 6803 via homologous recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH23
FEATURES             Location/Qualifiers
     source          1..4716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(2135..2140,2141..2157,2158..2163)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2352..2885
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gccggtactg ccgggcctct tgcgggatat tacttgccat ggccagttcc cccagaccca
       61 ctgaacaaac caaacaattt tttcagcaat ggcaaaccta tcagcagttg gttgaccaca
      121 attacatggc ccatcggcaa attcaccagg cggtgggggc tttgattcaa caacagttcg
      181 atcgcccgtt tgattttttg gaccttggct gtggggatgc ccaggcgatc gccaaaactt
      241 tgacggggag taaaatcaat tcctatacag gggtagattt atcccccaat gccctggccc
      301 tagcccagaa aaatttggac ccttttcgac aggtggagct aagggaaacg gattttttga
      361 cctatctggg tgaagccaac caaaccgccc cagccagctt tgatctgatt cacgtcggtt
      421 tttctttgca ccatctgctc ccagaagaaa aggaacaatt ttttgcccat tgcttttctc
      481 tgctgcgccc tgggggatgc ctagttatct acgacgtttt tcgtcagccc gagcaaaatc
      541 gacagcagta tattgaagcc tatttagcta aggccgaaag tcaatggact gcccttgccc
      601 ccgaccaact ccaggcgatc gctgcccata taaaggagtg cgatttcccc gaattggtgg
      661 ctaccatggc agaaattggc caaaaagtag gatttatacc accccaagtc ctctttactg
      721 agcctagggg tttccaccac tgctttgcct ttgccattag tcaaagtgga aaagccctgg
      781 cccaagacgg ggtgtaaaaa gcaattttcc gataatatgg gcagaagcta aacagtgccc
      841 caatatggga agctgtgggc cgtaaacatt caatccgaat aataaaggac tggttgaaga
      901 tggaagaaca aaagacagca actgccggga ttaaaaccga tgtaggacca atcactactc
      961 ccaatcccca aaagtcccca atcaccgacc aggcttggca ggaatggcta caaccggtgt
     1021 gggaagtgct gggtaaaatt cctcaaatga ccggggagtt tttcgaggac aacaagcaac
     1081 ccctcatttc cctgggcata atcctgttgg gcattatcag cgtcaaaatc ttgattgctg
     1141 tgctcgatgc cattaatgat attcctttat tggcaccaac tttacagttg atcggcatgg
     1201 gctacaccgc ttggtttatc tggcgttacc tttggaaagc tgaaaagcgc caagagttgg
     1261 cttccgaatt tggtgcacta aaggagcaaa tttttggcgg tggaagcggc agtggtatgg
     1321 tttcgaaggg cgaagaactg tttacgggag tggtgccgat cctggttgag ttggatggcg
     1381 acgtgaacgg acacaagttt agtgtctccg gcgaaggtga gggtgacgct acctacggta
     1441 aactgacgct gaaattcatt tgtactaccg gtaaactccc ggttccatgg cctacgctgg
     1501 ttacgaccct cagctggggt gtccaatgct tcgcccgcta cccggaccac atgaagcagc
     1561 acgacttttt caaaagtgcc atgcctgagg ggtacgttca agaacgcacc attttcttta
     1621 aggatgacgg caattacaaa acacgcgcag aggtgaaatt tgaaggtgat acgttggtca
     1681 atcgaatcga actcaaaggc attgacttta aagaggatgg caatatcctc ggccacaaac
     1741 tggaatacaa ttacttcagc gacaacgtct atatcacagc tgataagcag aaaaatggga
     1801 ttaaagccaa ctttaagatc cggcataaca ttgaagatgg cggcgtgcaa ctggcggatc
     1861 attatcagca gaatactccc atcggcgatg ggcccgtcct gttgccagat aaccattatc
     1921 tgtctaccca aagcaagttg tcgaaggatc ccaacgagaa gcgtgatcat atggtcctct
     1981 tggagttcgt gactgcggca ggcattaccc tcgggatgga tgaattgtat aagtaggctc
     2041 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
     2101 cacaccgtgg aaacggatga aggcacgaac ccagttgaca taagcctgtt cggttcgtaa
     2161 actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2221 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
     2281 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2341 gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2401 caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag
     2461 tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct
     2521 actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca
     2581 tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc
     2641 ccaggtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc
     2701 accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc
     2761 ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct
     2821 atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca
     2881 cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattgt
     2941 cacaacgccg ctgtctccag accgccccag cctctttttc ctgcgcctat cctccgggtt
     3001 tgatgttcgg gggatttttt ctggcccatg accaaccgtc ccgtcccatc ggtgtgggat
     3061 agttctccct tcattggtat tctaggagtt agtttttcgc cattcttcct cccctttttt
     3121 caggagcgat gagcaactcc ccgtctgttc ttacagagct attgcaggct ttacccaatc
     3181 tgcgggccca aacctacttc aagtcttccc tcacggcctt gtcccacgcc atggaagacc
     3241 aggtactagc ggaaagtaca gcgggcaatc ccctgatcat tgcggctttt cagcgggaga
     3301 gattttatcg tcaagaagcc caccgttatc gacgtattgc cgataagagc agtcaggttt
     3361 acgtgctctc cgctccggag acggaattta aacacaactc cggcatttac gaaaccattg
     3421 cctttactcc tgaagacagc ctcgcccagg aatggcattt gattgtcatt ggcgatcgtt
     3481 atgccagttg tctaatttgt cgagaacgac cgacccagga taaatgggat agtaaccgcc
     3541 gttttgaagg tgtttggacc tttgatcgcc aggtgagtcg agttgcagcc caactattgc
     3601 tacaacggat tttagcctac cgtccagagt tacaagccaa aatcgaggag gcgacccaaa
     3661 cctggctcca gccttcctgc actgttcccc cccggggcat tgacgataac cctgatccct
     3721 ttgtacaacg gttaatcacc tatctccaag ctggacagta taagttaatc aaagccaatc
     3781 gtaatttgca ggacaaagaa gagcgggaga gactgcttaa ctccatcaca ggggcggtgc
     3841 gacgttccct tgatcccgag gaaattatgc aggtggcggt ggaaaaacta ggccaaggtt
     3901 tgggcatttg ccgttgtgtg atttaccgct gtagtgctga tgatacagtg gcgaccatta
     3961 accatgaatt cctcggtagc ggtgttggtt ccatcaaggg ttatccctgg cccctcaaag
     4021 ataatcccct ttttcaggag gtggtggtcc tcaaggaggc gatcgccatc gacaacatcg
     4081 accgagatcc aaggttaggg gaaccggagc cttctacggg ggaagtgaaa accctggtga
     4141 agagttgtgc cattctgtcc tggatgttag tgccaatttt ttatcgggaa aggttgctgg
     4201 gcatgatgga atttcaccac tgtggtcctg cccccacgga gtgggaggac gatgccctta
     4261 ccctagtgga aggggtggcc acccaaattg gggtagcact gatccaagca gaatcctatg
     4321 ccaacctaca ggaattgaat gagcaattag ctgccctaga ccgcactcgg tctaacctgg
     4381 tggccattac agggcatgaa ctacgcaccc ccctttccac tatccaagtg tgtttggaaa
     4441 gcctggccac ggagccggat atgccccagg aattgagaca ggtaatgttg aacactgccc
     4501 tggaagatgc ggagaggatg cgcaaactgg tgcaggactt tctcacgcta tctcaactgg
     4561 aaagtggacg agtggaatgg aacgcagagc caatttccct agaagaatgt gtggagcttt
     4621 ccttgagcca tatccgagcc cacaatcggg gccaggaagt acctgtcatt gtcaatcagg
     4681 tgaatggccg ctatatgcgt tgatgcaatt tctatg
//
