LOCUS       sequence-202345-        4248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Copy number inducible plasmid. Contains mini-F origin for 
            maintenance and P1 lytic replication machinery under CopA promoter 
            for copy increase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Sheth RU, Yim SS, Wu FL, Wang HH
  TITLE     Multiplex recording of cellular events over time on CRISPR 
            biological tape.
  JOURNAL   Science. 2017 Dec 15;358(6369):1457-1461. doi: 
            10.1126/science.aao0958. Epub 2017 Nov 23.
  PUBMED    29170279
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aao0958"; journalName: "Science"; date: 
            "2017-12-15- 15"; volume: "358"; issue: "6369"; pages: "1457-1461"
COMMENT     Sequence Label: pTrig-CopA
FEATURES             Location/Qualifiers
     source          1..4248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        473..522
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             553..564
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(1704..1723)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1715..2530
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1715..2530
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1788..1807)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2618..2636
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2640..2683)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2688..2705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2842..2863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    2947..3197
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3200..3955)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      join(4046..4248,1..17)
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
ORIGIN
        1 aagtggataa ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg
       61 ctgttccctg gtgcttcctc gctcactcga aattcccggg gatagcttta tgcttgtaaa
      121 ccgttttgtg aaaaaatttt taaaataaaa aaggggacct ctagggtccc caattaatta
      181 gtaatataat ctattaaagg tcattcaaaa ggtcatccac cggatcaatt cccctgctcg
      241 cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc
      301 tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc
      361 tcactgatcc ggacttttac ccgcctggtt tattaatttc ttgaccttcc ccttgctgga
      421 aggtttaacc tttatcacag ccagtcaaaa ctgtcttaaa ggagtgtttt agctgtcacc
      481 ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg
      541 tttaatctag agaaagagga gaaatactag atgctggcta aagtcacttt cctgagctgt
      601 ataacgatga gcgattttac tttttctggc tatgaattgg cctgctttgt aacacactcc
      661 ggtctatccc gtagcgccgg gcatatcctg tcgcaatgtg caaatctcgc ggcaacaacc
      721 agtgaatact tcattcacaa gcctcaccgc ctgatcgcgg cagaaactgg ttatagccaa
      781 tcaaccgtcg ttcgtgcatt ccgtgaagct gtaaacaaag gtatcctgtc tgtagagatt
      841 gttatcggcg atcaccgtga acgtcgcgct aacctgtacc ggtttacacc atcctttttg
      901 gccttcgcac aacaagccaa aaatgcgctg atagaaagca aattaaagat ctcttcagcg
      961 gcaaccaagg ttaaagctgt tctcgctaag acattggctt tatttaattt tttatccaca
     1021 cccccatgtc aaaatgatac cccctccccc tgtcaggatg acgtggcaat aaagaataag
     1081 aagtcacaag ttaaaaaaac aaaaagatca gtttccggcg gtgccggaac aaccagcctc
     1141 aaaaaattga cttcatggat cgctaaggca aaagcaaagg ctgacaatct gcggttatcc
     1201 aaaaaacgca ctcaaaaaca tgagttcaag cagaaagtag aggcggctgc gcggaaatat
     1261 gcttacctga agaacaagcg ttcgcctgat attggcggga tatcaaactt cgataaccta
     1321 ccgcattgca tgacggtaaa cgaagctctt aatgcggttt tagccaaaaa taaagataac
     1381 gaacaatggg gtataccggc aggattcaga gggtaatgaa ttgctctaat tataaccatg
     1441 catactttca acacctctag tttgccatga ggcaaactca taggtgtcct ggtaagagga
     1501 cactgttgcc aaaactggac gccccattat tgcaattaat aaacaactaa cggacaattc
     1561 tacctaacaa taagtggagt tgcggccggc cgcgatccgc tagcaaagcc acgttgtgtc
     1621 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1681 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1741 ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1801 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
     1861 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1921 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1981 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
     2041 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2101 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2161 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2221 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaactgt tgccattctc
     2281 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2341 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2401 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2461 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2521 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cgagctctgc cacctgacgt
     2581 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     2641 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggcctc gctcactgac
     2701 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaatg
     2761 gcgcgccacg cgtacccata atacccataa tagctgtttg ccatcgcgta tgcatcgatc
     2821 acgtgtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcggatcga
     2881 tccggcgcgc accgtgggaa aaactccagg tagaggtaca cacgcggata gccaattcag
     2941 agtaataaac tgtgataatc aaccctcatc aatgatgacg aactaacccc cgatatcagg
     3001 tcacatgacg aagggaaaga gaaggaaatc aactgtgaca aactgccctc aaatttggct
     3061 tccttaaaaa ttacagttca aaaagtatga gaaaatccat gcaggctgaa ggaaacagca
     3121 aaactgtgac aaattaccct cagtaggtca gaacaaatgt gacgaaccac cctcaaatct
     3181 gtgacagata accctcagac tatcctgtcg tcatggaagt gatatcgcgg aaggaaaata
     3241 cgatatgagt cgtctggcgg cctttctttt tctcaatgta tgagaggcgc attggagttc
     3301 tgctgttgat ctcattaaca cagacttgca ggaagcggcg gcggaagtca ggcatacgct
     3361 ggtaactttg aggcagctgg taacgctcta tgatccagtc gattttcaga gagacgatgc
     3421 ctgagccatc cggcttacga tactgacaca gggattcgta taaacgcatg gcatacggat
     3481 tggtgatttc ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt aacccgataa
     3541 agaagggaat gagatatggg ttgatatgta cactgtaaag ccctctggat ggactgtgcg
     3601 cacgtttgat aaaccaagga aaagattcat agcctttttc atcgccggca tcctcttcag
     3661 ggcgataaaa aaccacttcc ttccccgcga aactcttcaa tgcctgccgt atatccttac
     3721 tggcttccgc agaggtcaat ccgaatattt cagcatattt agcaacatgg atctcgcaga
     3781 taccgtcatg ttcctgtagg gtgccatcag attttctgat ctggtcaacg aacagataca
     3841 gcatacgttt ttgatcccgg gagagactat atgccgcctc agtgaggtcg tttgactgga
     3901 cgattcgcgg gctattttta cgtttcttgt gattgataac cgctgtttcc gccatgacag
     3961 atccatgtga agtgtgacaa gtttttagat tgtcacacta aataaaaaag agtcaataag
     4021 cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca gggttaaggg
     4081 caatttgtca cagacaggac tgtcatttga gggtgatttg tcacactgaa agggcaattt
     4141 gtcacaacac cttctgtaga accagcatgg ataaaggcct acaaggcgct ctaaaaaaga
     4201 agatctaaaa actataaaaa aaataattat aaaaatatcc ccgtggat
//
