LOCUS       sequence-202357-       11246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of unphosphorylatable variant of RpaA (D53A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::RpaA D53A
FEATURES             Location/Qualifiers
     source          1..11246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(59..64,65..81,82..88)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     101..123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(960..977)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1130..1216
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1308..1335
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        complement(join(3214..3219,3220..3236,3237..3242))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3269..3287)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3355..3459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3460..3528,3529..4320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3678..3697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4491..5079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4980..4999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5233..5250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5265..5405
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5331..5350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(5507..5698)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     7156..7175
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(8265..8798)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8987..8992,8993..9009,9010..9015))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      9389..9475
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9567..9594
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        9913..9990
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9991..11073
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(10010..10029)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt
       61 gacaattaat catccggctc gtataatgtg tggaattgtg agcggataac aatttcacac
      121 aggaaacaga ccatggaatt catgaaaccc cgcatcctcg tgatcgatga tgactcagcc
      181 atcttggagc tggtcgccgt caatctggag atgtctggct atgacgtacg caaagctgag
      241 gacggcatta aaggtcaggc tttagctgtt cagctagttc ccgacctgat catgctggct
      301 ctaatgctgc cgcgggttga tggctttacc gtctgtcagc gactgcggcg cgatgagcgt
      361 actgccgaaa ttccggtgct gatgctgacc gccctcggac agactcagga taaggttgaa
      421 ggcttcaacg cgggtgctga cgattatctg actaagccct tcgaagttga agagatgctg
      481 gcccgcgtgc gtgccttgct gcggcgcacc gatcgcattc cccatgcagc ccgccatagc
      541 gaaattctca gctacggtcc gctgaccctg attcccgagc ggtttgaggc catttggttc
      601 aaccgcacgg tcaagctgac tcacttggaa tttgagttgt tgcactgcct gttgcaacgc
      661 cacggccaaa cggttgcgcc gagcgaaatc ctcaaagaag tctggggcta tgatcccgac
      721 gatgacatcg agacgattcg cgtccacatc cgtcatctgc gcaccaagct cgagcccgat
      781 ccccggcacc cgcgctacat caaaacggtc tatggagcgg gctactgcct tgagctgccg
      841 gccgagacgg aactccacca acacgccgat cagtttcctt cggcgtcctg aggatcctct
      901 agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc
      961 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
     1021 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     1081 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     1141 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1201 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     1261 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     1321 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     1381 atatgtatcc gctcatggcg gcccagatcc gcggccgccg atcctctagt atgcttgtaa
     1441 accgttttgt gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt
     1501 agtaatataa tctattaaag gtcattcaaa aggtcatcca ccggatcagc ttagtaaagc
     1561 cctcgctaga ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa
     1621 aaagccagcc tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa
     1681 aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat
     1741 ctaacgcttg agttaagccg cgcgggtcta gaggcctgtc gacgatatcg gttacccgtg
     1801 acgagtcagc tatagccaga agcacaactt tgccaatgga gagaacaatc gggacgggac
     1861 caatgacaac tacagctgga actacggcat tgaaggagag accgatgacc ccacgattct
     1921 gagcttacgg gaacggcagc agcgcaattt gctcgccacg ttattcctcg cccagggcac
     1981 accgatgctg acgatgggcg atgaggtcaa acgcagtcag cagggtaaca ataacgccta
     2041 ctgccaagac aatgagatca gctggtttga ttggtcgctg tgcgatcgcc atgccgattt
     2101 cttggtgttc agtcgccgcc tgattgaact ttcccagtcg ctggtgatgt tccaacagaa
     2161 cgaactgctg cagaacgaac cccatccgcg tcgtccctat gccatctggc atggcgtcaa
     2221 actcaaacaa cccgattggg cgctgtggtc ccacagtctg gccgtcagtc tctgccatcc
     2281 tcgccagcag gaatggcttt acctagcctt taatgcttac tgggaagacc tgcgcttcca
     2341 gttgccgagg cctcctcgcg gccgcgtttg gtatcgcttg ctcgatactt cactgccgaa
     2401 tcttgaagct tgtcatctgc cggatgaggc aaaaccctgc ctacggcgcg attacatcgt
     2461 cccagcgcga tcgctcttac tgttgatggc tcgtgcttaa aaacaatgca aacttcaccg
     2521 tttcagctgg tgattttcga ctgtgatggt gtgcttgttg atagcgaacg catcactaat
     2581 cgcgtctttg cagacatgct caatgaactg ggtctgttgg tgactttgga tgacatgttt
     2641 gagcagtttg tgggtcattc catggctgac tgtctcaaac taattgagcg acggttaggc
     2701 aatcctccac cccctgactt tgttcagcac tatcaacgcc gtacccgtat cgcgttagaa
     2761 acgcatctac aagccgttcc tggggttgaa gaggctttgg atgctcttga attgccctac
     2821 tgtgttgcgt ccagtggtga tcatcaaaag atgcgaacca cactgagcct gggatccaca
     2881 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     2941 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
     3001 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
     3061 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     3121 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     3181 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
     3241 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3301 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3421 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3481 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3541 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3601 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3661 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     3721 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3781 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3841 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3901 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3961 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     4021 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4081 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4141 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4201 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4261 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4321 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4381 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4441 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4501 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4561 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4621 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4681 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4741 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4861 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5101 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5161 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5221 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     5281 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     5341 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     5401 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5461 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5521 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     5581 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     5641 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     5701 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     5761 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     5821 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     5881 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     5941 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     6001 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     6061 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
     6121 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc
     6181 tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc
     6241 aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag
     6301 tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca
     6361 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc
     6421 caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat
     6481 gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc
     6541 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
     6601 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
     6661 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
     6721 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
     6781 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
     6841 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     6901 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
     6961 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     7021 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     7081 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     7141 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     7201 ggtgatgccg gccacgatgc gtccggcgta gaggatccga tcgccatcca cgcacaccct
     7261 acgctcgcac agtaatctat gagctgcatg ttggcggctt caccaagcat cccaattccg
     7321 gcgtcgcccc tgaaaaacgt ggcacctacg ctggtctaat cgaaaaaatt ccctacctgc
     7381 aatccctcgg cgtcacggcc gttgagttgc tgccggtgca ccagttcgat cgccaagatg
     7441 cccccttagg acgcgagaac tactggggct acagcaccat ggcttttttt gcgccccacg
     7501 cagcctacag ctctcgccat gatccacttg gtccagttga tgagttccgc gacctcgtca
     7561 aggcgctcca ccaagcaggg attgaggtga ttctcgacgt ggtgttcaac cacactgctg
     7621 aagggaatga agacggtcca acgctgtctt tcaaaggtct agcgaattca acctactatc
     7681 tgctggatga acaggcgggc tatcgcaact acaccggctg cggcaacacc gtcaaagcta
     7741 acaattcgat cgtgcgatcg ctgattctcg attgcctgcg ttattgggtc tcggaaatgc
     7801 acgtcgatgg cttccgcttt gaccttgcgt cggtgctgag tcgtgatgcc aatggcaacc
     7861 ccctatcgga tccgcccttg ctttgggcga ttgattccga tccggttttg gccggtacga
     7921 agctcattgc tgaagcttgg gacgcagccg gcttatatca ggttggtacc tttattggcg
     7981 atcgctttgg gacttggaac ggtcccttcc gggacgatat tcggcgtttt tggcgtggag
     8041 atcagggctg tacttacgcc ctcagtcaac gcctgctggg tagccccgat gtctacagca
     8101 cagaccaatg gtatgccgga cgcaccatta acttcatcac ctgccatgac ggctttacgc
     8161 tgcgagatct ccgcgctggg gttaccagat ccactagtgc ggcgttgtga caatttaccg
     8221 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     8281 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     8341 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     8401 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     8461 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     8521 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     8581 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     8641 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     8701 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     8761 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
     8821 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
     8881 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     8941 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
     9001 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcactagtg
     9061 gatctaagct tgcttctttg ctgacgagtg gcggacgggt gagtaatgtc tgggaaactg
     9121 cctgatggag ggggataact actggaaacg gtagctaata ccgcataacg tcgcaagacc
     9181 aaagaggggg accttcgggc ctcttgccat cggatgtgcc cagatgggat tagctagtag
     9241 gtggggtaac ggctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     9301 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     9361 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     9421 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     9481 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     9541 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     9601 tctacaaact ccggatccgg ccggatccgg ccaagcttgc tagctcgagc ccgcgacatc
     9661 ttcctgctcc agaagctcga aattcgagct cggtacccgg ggatctgggc cgcgggtcat
     9721 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     9781 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     9841 accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca
     9901 tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
     9961 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
    10021 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
    10081 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
    10141 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
    10201 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
    10261 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
    10321 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
    10381 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
    10441 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
    10501 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
    10561 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
    10621 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
    10681 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
    10741 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
    10801 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
    10861 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    10921 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
    10981 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11041 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagcg
    11101 cgaattgatc tggtttgaca gcttatcatc gactgcacgg tgcaccaatg cttctggcgt
    11161 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
    11221 gtcgctcaag gcgcactccc gttctg
//
