LOCUS       sequence-202361-        1594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 upon induction with doxicycline..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, 
            Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, 
            Menendez P
  TITLE     Generation and characterization of a human iPSC cell line expressing
            inducible Cas9 in the ""safe harbor"" AAVS1 locus.
  JOURNAL   Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. 
            Epub 2017 Apr 22.
  PUBMED    28677529
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-05"; volume: "21:137-140. doi"; pages: " 
            10.1016/j.scr.2017.04.011. Epub 2017 Apr 22"
COMMENT     Sequence Label: pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
FEATURES             Location/Qualifiers
     source          1..1594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(128..136,137..172,173..175,176..211,
                     212..214,215..250,251..256,257..874))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(947..966)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 anngcngcat aaacagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcgattta cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca
      181 tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa
      241 gggcgtcggc cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta
      301 gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt
      361 gtctcagaag tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt
      421 gatgctcctg ttcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt
      481 acagtgcgtt ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg
      541 tttcgtactg tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg
      601 agaggagagc acagcggaat gacttggcgt tgttccgcag aaagtcttgc catgactcgc
      661 cttccagggg gcagaagtgg gtatgatgcc tgtccagcat ctcgattggc agggcatcga
      721 gcagggcccg cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt
      781 gagcgagttt ccttgtcgtc aggccttcga taccgactcc attgagtaat tccagagcgc
      841 cgtttatgac tttgctcttg tccagtctag acatggtgaa ttcctgcagc ccgggggatc
      901 cactagcttc gatcaagccg aattctttgc caaaatgatg agacagcaca ataaccagca
      961 cgttgcccag aagctgtaag aaaaagaaaa aggcatgaac atggtnagca aaaggctcta
     1021 gaaccgcccg gtcacacgcc aaaaagccga aaccccgccc tggccccgtc ccccccgaaa
     1081 ggcagccgtc ccccccgcgg aacngccccc aaggctggaa aagggaaaaa gggnaacggc
     1141 cgccccggcc aacccacgaa aggcccttcc ccgcccattt tctttcctgc nggggccnna
     1201 accgctttcn cccccgggcc ccgctaaaag ggggtgccgg ccggcccctc cccaaaattt
     1261 ccggctccnc nacaaaattt gggaacaaaa ggaaattccc nnncccccct cctcnnanna
     1321 attacnaaaa aaagggcaaa tggctgccgg nnnnccccnc cntccaaaan cccgccgggc
     1381 cttccggggg ggccnggngn cccccctccc ccgnaaagnn nnnccngggg cccagnngnn
     1441 nnccccnntc ccnnnccggg naaaccccna antttgnncc cnnaccccct ngccgggggn
     1501 nggnnnnanc ctnnnnnnnc cncnngccct ttaaagnaaa nnnncnnncc ngaaaaacgc
     1561 gcgccnnnnc ncnannannn ncngggnnnn gggg
//
