LOCUS       sequence-202362-        1753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 upon induction with doxicycline..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, 
            Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, 
            Menendez P
  TITLE     Generation and characterization of a human iPSC cell line expressing
            inducible Cas9 in the ""safe harbor"" AAVS1 locus.
  JOURNAL   Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. 
            Epub 2017 Apr 22.
  PUBMED    28677529
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-05"; volume: "21:137-140. doi"; pages: " 
            10.1016/j.scr.2017.04.011. Epub 2017 Apr 22"
COMMENT     Sequence Label: pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
FEATURES             Location/Qualifiers
     source          1..1753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(106..723,724..729,730..765,766..768,769..804,805..807,
                     808..843,844..852)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    962..1043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(999..1018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tnnnnnngct cngngcacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
       61 ggcttgatcg aagctagtgg atcccccggg ctgcaggaat tcaccatgtc tagactggac
      121 aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg
      181 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
      241 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
      301 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
      361 ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
      421 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
      481 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
      541 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
      601 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
      661 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
      721 ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
      781 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
      841 ctccccgggt aaatcgatca taatcagcca taccacattt gtagaggttt tacttgcttt
      901 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      961 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1021 aaataaagca tttttttcac tgcctcgacc cataaagccc accgcatccc cagcatggct
     1081 ggctattgtc ttcccaatcc tcccccttgc tgtcctggcc canccccacc ccccagaann
     1141 agaatgacac cctactccan ncaatggcga tggcaatttc cctcattttt attaggaaaa
     1201 ggacannngg gaattggcac ctttccaggg tccaaggaaa ggcaccgggg gaaggggcca
     1261 annaacnaaa tggcttggca actaaaaagg ccacaaaccc gnnccnttga cctaaaggat
     1321 tccccggatn nacccccttt ggaaaaaaaa aagctggggc cnnnncnncc cacccntngg
     1381 aattctttaa ttttttcttt tttggccggg gcgggccttt tttannnggg cgcgggccct
     1441 tttttgcccc cccnccttnn aaaantggnn nntcccgggt nncccnaaag nnccgtggna
     1501 nnnnnnngnn naaacaaggn gagnnacccn ncnncccnnn nnnggnnggn nnnnncnnnc
     1561 tcccnncnna nannnnnggg ganaaaaaat tnnnannggg nncgngnnnn nccccnnnnn
     1621 nntnnnagnn nnnanctgtg nnnaannnnn cccnnanncc ncccccnnnn gnacnnnnna
     1681 actnnntgnn naaatnnaan nnncnaangn cnnnttannn ttattgnccc nggangnnta
     1741 cncntcttgn nna
//
