LOCUS       sequence-202373-        4578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty AAV vector with TBG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4578)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV.TBG.PI.Null.bGH
FEATURES             Location/Qualifiers
     source          1..4578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    27..48
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(63..68,69..86,87..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    101..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     106..128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     125..141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     125..141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        648..1057
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin 
                     gene (Yan et al., 2012)"
     intron          1117..1249
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1232..1251
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(1505..1522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1511..1718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1806..1935
                     /label=AAV2 ITR
     primer_bind     complement(1954..1971)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1954..1970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1963..1985)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2112..2567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2199..2218)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2409..2430
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2593..2697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2698..2766,2767..3558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2916..2935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3729..4317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4218..4237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
       61 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      121 cacacaggaa acagctatga ccatgattac gccagattta attaaggcct taattaggct
      181 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
      241 ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
      301 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
      361 gaagatcgga attcgccctt aagctagcag gttaattttt aaaaagcagt caaaagtcca
      421 agtggccctt ggcagcattt actctctctg tttgctctgg ttaataatct caggagcaca
      481 aacattccag atccaggtta atttttaaaa agcagtcaaa agtccaagtg gcccttggca
      541 gcatttactc tctctgtttg ctctggttaa taatctcagg agcacaaaca ttccagatcc
      601 ggcgcgccag ggctggaagc tacctttgac atcatttcct ctgcgaatgc atgtataatt
      661 tctacagaac ctattagaaa ggatcaccca gcctctgctt ttgtacaact ttcccttaaa
      721 aaactgccaa ttccactgct gtttggccca atagtgagaa ctttttcctg ctgcctcttg
      781 gtgcttttgc ctatggcccc tattctgcct gctgaagaca ctcttgccag catggactta
      841 aacccctcca gctctgacaa tcctctttct cttttgtttt acatgaaggg tctggcagcc
      901 aaagcaatca ctcaaagttc aaaccttatc attttttgct ttgttcctct tggccttggt
      961 tttgtacatc agctttgaaa ataccatccc agggttaatg ctggggttaa tttataacta
     1021 agagtgctct agttttgcaa tacaggacat gctataaaaa tggaaagatg ttgctttctg
     1081 agagactgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
     1141 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
     1201 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccaggcg
     1261 gccaattcga atggccatgg gacgtcgacc tgaggtaatt ataacccggg ccctatatat
     1321 ggatccaatt gcaatgatca tcatgacaga tctgcgcgcg atcgattggc cacgtgggcc
     1381 gtgcacctta agcttatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
     1441 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataagga
     1501 tctgcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1561 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1621 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1681 ggggaggatt gggaagacaa tagcaggcat gctggggact cgagttaagg gcgaattccc
     1741 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
     1801 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     1861 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     1921 cgagcgagcg cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga
     1981 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2041 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2101 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2161 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2221 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2281 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2341 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2401 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2461 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2521 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     2581 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2761 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2821 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2881 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2941 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3001 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3061 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3121 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3181 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3241 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3301 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3361 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3421 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3481 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3541 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3601 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3661 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3721 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3781 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3841 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3901 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3961 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4081 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4141 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4261 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4321 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4381 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4441 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4501 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4561 gacaggtttc ccgactgg
//
