LOCUS       sequence-202374-        9600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac plasmid encoding EYFP in an inverse orientation flanked by 
            loxP and lox2722 sites under the control of the EF1a promotor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, 
            Busskamp V
  TITLE     On-demand optogenetic activation of human stem-cell-derived neurons.
  JOURNAL   Sci Rep. 2017 Oct 31;7(1):14450. doi: 10.1038/s41598-017-14827-6.
  PUBMED    29089561
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-31"; volume: "7(1):14450. doi"; pages: " 
            10.1038/s41598-017-14827-6"
COMMENT     Sequence Label: PB-eDIO-EYFP
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..127)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     318..337
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     551..573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     565..582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     566..582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   712..774
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1275..2453
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1506..2444
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2401..2421
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(2484..2517)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2568..2601)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(2637..3347,3348..3350,3351..3353))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2679..2700)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3028..3047
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3287..3308
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3350..3359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3364..3397
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3448..3481
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3495..4083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3548..3568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3966..3977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4090..4314
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4326..5537
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5374..5393
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5567..5576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5573..5971
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYAWEG"
     polyA_signal    6010..6131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6047..6066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6101..6120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6892..6926
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7417..7433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7417..7433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7430..7452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7441..7457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7465..7471,7472..7489,7490..7495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7510..7531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7648..7665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7819..8407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7899..7918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8578..9369,9370..9438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9201..9220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9439..9543)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(9569..9600,1..424))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
       61 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      121 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
      181 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
      241 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      301 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      361 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      421 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      481 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      541 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt
      601 cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg
      661 atggagcaga tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa
      721 gataatcata ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt
      781 atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt
      841 tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc
      961 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     1021 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     1081 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     1141 gctctttgag cctgcagaca cctgggggga tacggggaaa aggcctgtaa gctttgcaaa
     1201 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
     1261 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     1321 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     1381 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     1441 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     1501 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     1561 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     1621 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     1681 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     1741 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     1801 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     1861 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     1921 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     1981 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     2041 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     2101 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     2161 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     2221 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     2281 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     2341 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     2401 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggtaccg
     2461 gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg aagttatgca
     2521 gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata
     2581 gcatacatta tacgaagtta tggcgcgccc tattacacct cgttctcgta gcagaacttg
     2641 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
     2701 tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag gtagtggttg
     2761 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
     2821 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
     2881 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
     2941 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     3001 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     3061 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     3121 tggtcggggt agcgggcgaa gcactgcagg ccgtagccga aggtggtcac gagggtgggc
     3181 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     3241 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     3301 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggct
     3361 agcataactt cgtataaagt atcctatacg aagttatttg ccttaaccca gaaattatca
     3421 ctgttattct ttagaatggt gcaaagaata acttcgtata atgtatgcta tacgaagtta
     3481 tgaattcgga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3541 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3601 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3661 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3721 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3781 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3841 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3901 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3961 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4021 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4081 cgcgaattcc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4141 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4201 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4261 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggatcgat
     4321 gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     4381 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
     4441 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
     4501 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
     4561 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
     4621 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
     4681 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
     4741 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
     4801 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
     4861 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
     4921 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
     4981 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
     5041 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
     5101 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
     5161 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
     5221 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
     5281 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     5341 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     5401 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     5461 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     5521 tggctttttt gttagacgaa gcttgggctg caggtcgact ctagatgcca ccatggccaa
     5581 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     5641 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     5701 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     5761 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     5821 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     5881 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     5941 gctgccctct ggttatgcgt gggagggcta agtcgacaat caacctctgg attacaaaat
     6001 ttgtgggtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6121 aatgtatctt atcatgtctg gaattgactc aaatgatgtc aattagtcta tcagaagcta
     6181 tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc
     6241 aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac
     6301 aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt
     6361 ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct
     6421 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
     6481 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
     6541 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
     6601 tggggggggc tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt
     6661 aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa
     6721 aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt
     6781 accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc
     6841 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
     6901 attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt
     6961 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
     7021 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
     7081 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
     7141 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
     7201 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
     7261 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
     7321 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
     7381 gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7501 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7561 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7621 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7681 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7741 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7801 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7861 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7981 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8041 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8161 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8221 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8281 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8341 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8401 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8461 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8641 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8701 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8881 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9421 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9481 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9541 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
//
