LOCUS       sequence-202381-        5725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5725)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..5725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1711..2391
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2513..2634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2550..2569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2604..2623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2641..3096)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2778..2799)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2990..3009
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3123..3227
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3229..3586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3229..3420
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3252..3272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3437..3572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3621..4415
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3621..4415
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3675..3694)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4285..4304
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4603..4622)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4647..4694
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5023..5611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5512..5531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggct gagccccgcc aggagttcga agtgatggaa
      661 gatcacgctg ggacgtacgg gttgggggac aggaaagatc aggggggcta caccatgcac
      721 caagaccaag agggtgacac ggacgctggc ctgaaagctg aagaagcagg cattggagac
      781 acccccagcc tggaagacga agctgctggt cacgtgaccc aagctcgcat ggtcagtaaa
      841 agcaaagacg ggactggaag cgatgacaaa aaagccaagg gggctgatgg taaaacgaag
      901 atcgccacac cgcggggagc agcccctcca ggccagaagg gccaggccaa cgccaccagg
      961 attccagcaa aaaccccgcc cgctccaaag acaccaccca gctctggtga acctccaaaa
     1021 tcaggggatc gcagcggcta cagcagcccc ggctccccag gcactcccgg cagccgctcc
     1081 cgcaccccgt cccttccaac cccacccacc cgggagccca agaaggtggc agtggtccgt
     1141 actccaccca agtcgccgtc ttccgccaag agccgcctgc agacagcccc cgtgcccatg
     1201 ccagacctga agaatgtcaa gtccaagatc ggctccactg agaacctgaa gcaccagccg
     1261 ggaggcggga aggtgcaaat agtctacaaa ccagttgacc tgagcaaggt gacctccaag
     1321 tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga agtaaaatct
     1381 gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga caatatcacc
     1441 cacgtccctg gcggaggaaa taaaaagatt gaaacccaca agctgacctt ccgcgagaac
     1501 gccaaagcca agacagacca cggggcggag atcgtgtaca agtcgccagt ggtgtctggg
     1561 gacacgtctc cacggcatct cagcaatgtc tcctccaccg gcagcatcga catggtagac
     1621 tcgccccagc tcgccacgct agctgacgag gtgtctgcct ccctggccaa gcagggtttg
     1681 agctccggag gggatccacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc
     1741 aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
     1801 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
     1861 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccaaa
     1921 tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     1981 cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat aacaatggaa
     2041 ggggacactt tctataataa agttcgattt tatggtacca actttcccgc caatggtcca
     2101 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     2161 ggagtgctga cgggtgatat tgagatggct ttgttgcttg aaggaaatgc ccattaccga
     2221 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggcgcccac
     2281 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     2341 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg
     2401 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2461 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2521 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2581 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     2641 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     2701 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2761 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2821 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2881 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2941 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3001 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3061 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3181 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     3241 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3301 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3361 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3421 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3481 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3541 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     3601 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3661 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3721 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3781 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     3841 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3901 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3961 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4021 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4081 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4141 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4201 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4261 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4321 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4381 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4441 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4501 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4561 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     4621 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     4681 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     4741 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     4801 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     4861 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5161 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5701 ggataaccgt attaccgcca tgcat
//
