LOCUS       sequence-202407-       12200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  APRT c.407T>C (APRT*J) donor vector with unilateral microhomology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 12200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: pbG-APRT-J-u32uni-PdTKmCh
FEATURES             Location/Qualifiers
     source          1..12200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(20..448)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(130..151)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     342..361
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     575..597
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     589..606
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     590..606
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1456..1509
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1519..2115
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1519..1538)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2015..2035
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2122..3117
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3155..3379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3398..3777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3779..4056
                     /label=chicken beta-actin promoter
     intron          4057..5072
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    5166..5190
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5196..5906
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5341..5359)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5609..5628
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5803..5826
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5908..5932)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6032..6051)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6097..6152
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6151..6170)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        7077..7456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7459..7734
                     /label=chicken beta-actin promoter
     intron          7735..8743
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    8837..8861
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8867..8869,8870..8872,8873..9583)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8867..8869,8870..8872,8873..9583)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8912..8933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9173..9192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(9645..9669)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9684..9700)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9685..9701)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    9722..9946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(10002..10020)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10041..10057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10041..10057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10054..10076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10065..10081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10089..10095,10096..10113,10114..10119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10134..10155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10272..10289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10443..11031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10523..10542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11202..11993,11994..12062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11825..11844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12063..12167)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
       61 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      121 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      181 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      241 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      301 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      361 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      421 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
      481 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
      541 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      601 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccactcc tgtcacttac
      661 cctgacaggc ctagactccc gaggcttcct ctttggcccc tccctggccc aggagcttgg
      721 actgggctgc gtgctcatcc gaaagcgggg gaagctgcca ggccccactc tgtgggcctc
      781 ctattccctg gagtacggga aggtaagagg gctggggtgg ccagaggaag ggcagggcca
      841 ggccaccgtg gccactctcc cccagttcta aaaggccttc ccaggcgtgt caagtggagc
      901 tgctgtggtt acagtggcct tgggagctca gagaggttga gacataggct gggctcacac
      961 agccaggtaa cagcaaggtg gggttggagt cagggtctag ggtggcagct gccaagctgt
     1021 gcaacaaagc tgttttctgc gggaggctga ggaccacaca ccacttccca ctccaggctg
     1081 agctggagat tcagaaagac gccctggagc caggacagag ggtggtcgtc gtggatgatc
     1141 tgctggccac tggtggtaag ggtctccccg cagccaactg ctgtggctcc aagggcctgg
     1201 tgggagtggg acaggacctc gctgtgtgac atgggatgca gcttactgtt gtccagaggg
     1261 tgcctggtgg ccaggccgac accttcctct ccccatgcct tcccctcccc aacccagggg
     1321 ctggcctgga gcacctgctc tctgcagccc aggccaactg gggacctcac cctcccatcc
     1381 ccaggtacca cgaacgctgc ctgtgagctg ctgggctcca ccggcaagct gcccgtgccc
     1441 agatctggca gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     1501 cccggcccta ggctcgagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     1561 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     1621 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     1681 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     1741 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     1801 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     1861 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     1921 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     1981 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     2041 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     2101 cgcaagcccg gtgccggatc catgcccacg ctactgcggg tttatataga cggtccccac
     2161 gggatgggga aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc
     2221 gtctacgtac ccgagccgat gacttactgg cgggtgctgg gggcttccga gacaatcgcg
     2281 aacatctaca ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg
     2341 gtggtaatga caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt
     2401 ctggctcctc atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc
     2461 ctcatcttcg accgccatcc catcgccgcc ctcctgtgct acccggccgc gcggtacctt
     2521 atgggcagca tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg
     2581 cccggcacca acatcgtgct tggggccctt ccggaggaca gacacatcga ccgcctggcc
     2641 aaacgccagc gccccggcga gcggctggac ctggctatgc tggctgcgat tcgccgcgtt
     2701 tacgggctac ttgccaatac ggtgcggtat ctgcagtgcg gcgggtcgtg gcgggaggac
     2761 tggggacagc tttcggggac ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg
     2821 ggcccacgac cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg
     2881 gcccccaacg gcgacctgta taacgtgttt gcctgggcct tggacgtctt ggccaaacgc
     2941 ctccgttcca tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac
     3001 gccctgctgc aacttacctc cgggatggtc cagacccacg tcaccacccc cggctccata
     3061 ccgacgatat gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgatct
     3121 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3361 gatgcggtgg gctctatggg ctagcggtgg cggcctcgac attgattatt gactagttat
     3421 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3481 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3541 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3601 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3661 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3721 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     3781 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     3841 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
     3901 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
     3961 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     4021 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
     4081 cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     4141 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     4201 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     4261 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     4321 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     4381 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     4441 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     4501 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     4561 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     4621 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     4681 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     4741 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     4801 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     4861 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     4921 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     4981 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     5041 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     5101 tgtctcatca ttttggcaaa gaattcgcgg ccgcggtacc ggcgcgccgg atcctgcagg
     5161 ttatcacaag tttgtacaaa aaagcaggct ccaccatggt gagcaagggc gaggaggaca
     5221 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     5281 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5341 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc
     5401 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     5461 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5521 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     5581 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     5641 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     5701 ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc tacaaggcca
     5761 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagctggac atcacctccc
     5821 acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg
     5881 gcggcatgga cgagctgtac aagtaagacc cagctttctt gtacaaagtg gtgataactc
     5941 tagagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     6001 tgccctggct cacaaatacc actgagatcg atctttttcc ctctgccaaa aattatgggg
     6061 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     6121 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     6181 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc atatgctggc
     6241 tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     6301 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     6361 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     6421 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     6481 gacctgcagc ccaagcttgg gcacgggcag cttgccggtg gagtaccatg aacgctgcct
     6541 gtgagctgct gggccgcctg caggctgagg tcctggagtg cgtgagcctg gtggagctga
     6601 cctcgcttaa gggcagggag aagctggcac ctgtaccctt cttctctctc ctgcagtatg
     6661 agtgaccaca gggcctccca gcccaacatc tccagctgga tcccagggaa atatcagcct
     6721 tgggcaactg cagtgaccag gggcaccggc tgcccacagg gaacacattc ctttgctggg
     6781 gttcagcgcc tctcctgggg ctggaagtgc caaagcctgg ggcaaagctg tgtttcagcc
     6841 acactgaacc caattacaca cagcgggaga acgcagtaaa cagctttccc acaagagccg
     6901 tctcctgtcc tcctgttccc cagggcaggg agccccagga caacaccaga cttcagctgt
     6961 actgtgggca tgtgctgctt ggcgtgatgc ccagcagaac ctgtcctggt tctcacctca
     7021 ggagctagaa ccctccaggg taccggccat gatggccgaa ttaattcaag ctagtcgaca
     7081 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     7141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     7201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     7261 ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt
     7321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     7381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     7441 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     7501 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     7561 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     7621 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     7681 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     7741 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     7801 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     7861 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     7921 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     7981 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     8041 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     8101 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     8161 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     8221 gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc
     8281 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     8341 gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg
     8401 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     8461 tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     8521 cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     8581 cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg gggacggctg
     8641 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     8701 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     8761 ggttattgtg ctgtctcatc attttggcaa agaattggat cctagtggat cccccgggct
     8821 gcaggaattc gatatcacaa gtttgtacaa aaaagcaggc tggaccatgg tgagcaaggg
     8881 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     8941 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     9001 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     9061 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     9121 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     9181 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     9241 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     9301 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     9361 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     9421 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     9481 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     9541 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggtg gaagatctgg
     9601 gaattcaagg cctctcgagc ctctagattc tgcagcccta tagcacccag ctttcttgta
     9661 caaagtggtg atatcaagct tatcgatacc gtcgacctcg agggccgctg atcagcctcg
     9721 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     9781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     9841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     9901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     9961 agaaccagct ggggcttaat taagagctcc agcttttgtt ccctttagtg agggttaatt
    10021 tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10081 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10141 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10201 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    10261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10621 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10861 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    10921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    11161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    11221 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    11281 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    11341 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    11401 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    11461 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    11521 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    11581 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    11641 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11701 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11761 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11821 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11881 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11941 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    12001 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    12061 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    12121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    12181 aaagtgccac ctaaattgta
//
