LOCUS       sequence-202416-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the expression of the construct alpha-Synuclein-GFP, 
            which contains N-terminal tags, which can be cleaved later: His-tag 
            for purification and SUMO-tag for better solubility.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA
  TITLE     Modification of C Terminus Provides New Insights into the Mechanism 
            of alpha-Synuclein Aggregation.
  JOURNAL   Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 
            10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
  PUBMED    28939194
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2017.08.027"; journalName: "Biophys J"; date: 
            "2017-11-21- 21"; volume: "113"; issue: "10"; pages: "2182-2191"
COMMENT     Sequence Label: 1a. pETM11-SUMO-SNCA-GFP
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(457..459,460..1173)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(457..459,460..1170)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(499..520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(760..779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1107..1128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggatgtat tcatgaaagg actttcaaag gccaaggagg gagttgtggc tgctgctgag
       61 aaaaccaaac agggtgtggc agaagcagca ggaaagacaa aagagggtgt tctctatgta
      121 ggctccaaaa ccaaggaggg agtggtgcat ggtgtggcaa cagtggctga gaagaccaaa
      181 gagcaagtga caaatgttgg aggagcagtg gtgacgggtg tgacagcagt agcccagaag
      241 acagtggagg gagcagggag cattgcagca gccactggct ttgtcaaaaa ggaccagttg
      301 ggcaagaatg aagaaggagc cccacaggaa ggaattctgg aagatatgcc tgtggatcct
      361 gacaatgagg cttatgaaat gccttctgag gaagggtatc aagactacga acctgaagcc
      421 catatggcta gcggcggctc tggtggcggc tctggtgtga gcaagggcga ggagctgttc
      481 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      541 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      601 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      661 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      721 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      781 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      841 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      901 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      961 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1141 atcactctcg gcatggacga gctctacaag tga
//
