LOCUS       sequence-202417-        1497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the expression of the construct alpha-Synuclein-GFP, 
            which contains N-terminal tags, which can be cleaved later: His-tag 
            for purification and SUMO-tag for better solubility.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA
  TITLE     Modification of C Terminus Provides New Insights into the Mechanism 
            of alpha-Synuclein Aggregation.
  JOURNAL   Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 
            10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
  PUBMED    28939194
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2017.08.027"; journalName: "Biophys J"; date: 
            "2017-11-21- 21"; volume: "113"; issue: "10"; pages: "2182-2191"
COMMENT     Sequence Label: 1a. pETM11-SUMO-SNCA-GFP
FEATURES             Location/Qualifiers
     source          1..1497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(781..783,784..1497)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(781..783,784..1494)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(823..844)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1084..1103)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1431..1452
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgaaacatc accatcacca tcaccccatg agcgattacg acatccccac tactgagaat
       61 ctttattttc agggcgccat gggcaacgat cacattaacc tgaaagtggc cggtcaagac
      121 ggtagcgtag tccagtttaa aatcaaacgc cacacccctc tgtcgaaact gatgaaagcc
      181 tattgtgaac gccaaggtct gtctatgcgt cagatccgtt ttcgcttcga tggacagccg
      241 attaacgaaa ccgacactcc agcacagctg gaaatggaag atgaggacac cattgacgtg
      301 ttccagcaac agaccggtgg atccatggat gtattcatga aaggactttc aaaggccaag
      361 gagggagttg tggctgctgc tgagaaaacc aaacagggtg tggcagaagc agcaggaaag
      421 acaaaagagg gtgttctcta tgtaggctcc aaaaccaagg agggagtggt gcatggtgtg
      481 gcaacagtgg ctgagaagac caaagagcaa gtgacaaatg ttggaggagc agtggtgacg
      541 ggtgtgacag cagtagccca gaagacagtg gagggagcag ggagcattgc agcagccact
      601 ggctttgtca aaaaggacca gttgggcaag aatgaagaag gagccccaca ggaaggaatt
      661 ctggaagata tgcctgtgga tcctgacaat gaggcttatg aaatgccttc tgaggaaggg
      721 tatcaagact acgaacctga agcccatatg gctagcggcg gctctggtgg cggctctggt
      781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      841 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      901 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      961 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1021 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1081 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1141 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1201 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1261 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1321 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1381 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1441 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctcta caagtga
//
