LOCUS       sequence-202448-        7454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of Cre from GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7454)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV.GFAP.Cre.WPRE.hGH
FEATURES             Location/Qualifiers
     source          1..7454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1842..2873
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    2887..3475
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2940..2960)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3358..3369)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3507..3983
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3706..3725)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4023..4163
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4023..4152
                     /label=AAV2 ITR
     rep_origin      4238..4693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4325..4344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4535..4556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4710..4729)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4829..4851
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4889..4907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4975..5079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5080..5148,5149..5940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6111..6699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6600..6619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6761..6890
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    6906..7023
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaaaaaaaa aaagaacata tcctggtgtg gagtagggga cgctgctctg acagaggctc
       61 gggggcctga gctggctctg tgagctgggg aggaggcaga cagccaggcc ttgtctgcaa
      121 gcagacctgg cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat
      181 gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac
      241 cccagccccc ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc
      301 cagcctgaca gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg
      361 ctgtgtggcg ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg
      421 gatcagggga tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc
      481 ttcccagaag tccaaggaca caaatgggtg aggggactgg gcagggttct gaccctgtgg
      541 gaccagagtg gagggcgtag atggacctga agtctccagg gacaacaggg cccaggtctc
      601 aggctcctag ttgggcccag tggctccagc gtttccaaac ccatccatcc ccagaggttc
      661 ttcccatctc tccaggctga tgtgtgggaa ctcgaggaaa taaatctcca gtgggagacg
      721 gaggggtggc cagggaaacg gggcgctgca ggaataaaga cgagccagca cagccagctc
      781 atgtgtaacg gctttgtgga gctgtcaagg cctggtctct gggagagagg cacagggagg
      841 ccagacaagg aaggggtgac ctggagggac agatccaggg gctaaagtcc tgataaggca
      901 agagagtgcc ggccccctct tgccctatca ggacctccac tgccacatag aggccatgat
      961 tgacccttag acaaagggct ggtgtccaat cccagccccc agccccagaa ctccagggaa
     1021 tgaatgggca gagagcagga atgtgggaca tctgtgttca agggaaggac tccaggagtc
     1081 tgctgggaat gaggcctagt aggaaatgag gtggcccttg agggtacaga acaggttcat
     1141 tcttcgccaa attcccagca ccttgcaggc acttacagct gagtgagata atgcctgggt
     1201 tatgaaatca aaaagttgga aagcaggtca gaggtcatct ggtacagccc ttccttccct
     1261 tttttttttt ttttttttgt gagacaaggt ctctctctgt tgcccaggct ggagtggcgc
     1321 aaacacagct cactgcagcc tcaacctact gggctcaagc aatcctccag cctcagcctc
     1381 ccaaagtgct gggattacaa gcatgagcca ccccactcag ccctttcctt cctttttaat
     1441 tgatgcataa taattgtaag tattcatcat ggtccaacca accctttctt gacccacctt
     1501 cctagagaga gggtcctctt gcttcagcgg tcagggcccc agacccatgg tctggctcca
     1561 ggtaccacct gcctcatgca ggagttggcg tgcccaggaa gctctgcctc tgggcacagt
     1621 gacctcagtg gggtgagggg agctctcccc atagctgggc tgcggcccaa ccccaccccc
     1681 tcaggctatg ccagggggtg ttgccagggg cacccgggca tcgccagtct agcccactcc
     1741 ttcataaagc cctcgcatcc caggagcgag cagagccaga gcaggttgga gaggagacgc
     1801 atcacctccg ctgctcgccg ggatcgcggg aattcgccac catgtccaat ttactgaccg
     1861 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
     1921 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
     1981 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
     2041 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
     2101 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
     2161 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
     2221 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
     2281 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
     2341 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
     2401 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
     2461 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
     2521 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
     2581 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
     2641 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
     2701 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
     2761 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
     2821 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagccagctt
     2881 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2941 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3001 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3061 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3121 cccactggtt ggggcattgc caccacctgt cagctccttt ccggaacttt cgctttcccc
     3181 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3241 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3301 ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3361 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3421 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     3481 taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc
     3541 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     3601 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     3661 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     3721 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     3781 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     3841 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     3901 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     3961 ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc
     4021 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4081 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4141 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     4201 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     4261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     4321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4441 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4501 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4561 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     4621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4681 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4741 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4801 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4861 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5761 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6721 ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca gctgcgcgct
     6781 cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
     6841 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgcac
     6901 gcgtttttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     6961 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     7021 tttcgggttt attacaggga cagcagagat ccagtttggt taattaaccc acctccctct
     7081 ctgtgctggg actcacagag ggagacctca ggaggcagtc tgtccatcac atgtccaaat
     7141 gcagagcata ccctgggctg ggcgcagtgg cgcacaactg taattccagc actttgggag
     7201 gctgatgtgg aaggatcact tgagcccaga agttctagac cagcctgggc aacatggcaa
     7261 gaccctatct ctacaaaaaa agttaaaaaa tcagccacgt gtggtgacac acacctgtag
     7321 tcccagctat tcaggaggct gaggtgaggg gatcacttaa ggctgggagg ttgaggctgc
     7381 agtgagtcgt ggttgcgcca ctgcactcca gcctgggcaa cagtgagacc ctgtctcaaa
     7441 agacaaaaaa aaaa
//
