LOCUS       sequence-202452-        5028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of Cre from hSyn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5028)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENN.AAV.hSyn.Cre.hGH
FEATURES             Location/Qualifiers
     source          1..5028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        171..618
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             633..1664
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    1721..2197
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1920..1939)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2237..2377
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2237..2366
                     /label=AAV2 ITR
     rep_origin      2452..2907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2539..2558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2749..2770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2924..2943)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3043..3065
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3103..3121)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3189..3293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3294..3362,3363..4154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3512..3531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4325..4913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4814..4833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(4975..5028,1..76)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
       61 atcactaggg gttcctgcgg ccgcacgcgt tttttaaaag aaaagggggg attggggggt
      121 acagtgcagg ggaaagaata gtaggatctc tgcagagggc cctgcgtatg agtgcaagtg
      181 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca
      241 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
      301 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
      361 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
      421 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
      481 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
      541 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
      601 tcgtgcctga gagcgcagtc gaattcgcca ccatgtccaa tttactgacc gtacaccaaa
      661 atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
      721 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
      781 ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag
      841 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
      901 aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca
      961 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
     1021 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
     1081 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca
     1141 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
     1201 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
     1261 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
     1321 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
     1381 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
     1441 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
     1501 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
     1561 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
     1621 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagccatta acgcggcgtg
     1681 gtacctctag agtcgacccg ggcggcctcg agagatctac gggtggcatc cctgtgaccc
     1741 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     1801 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     1861 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     1921 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     1981 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     2041 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     2101 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     2161 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     2221 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2281 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2341 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     2401 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     2461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2521 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2641 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     2701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2761 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     2821 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2881 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     2941 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3001 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3061 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3121 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3301 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3361 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3421 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3481 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3541 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3601 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3661 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3721 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3781 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3841 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3901 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3961 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4021 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4081 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4141 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4201 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca
     4981 ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcg
//
