LOCUS       sequence-202453-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to 
            FKBP and TALE-VEGFA-TS3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Scot Wolfe
  TITLE     Inducible Cas9-DNA-binding-domain fusions for precise genome editing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TA...
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(109..111,112..432)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             1313..1324
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2842..2862
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2863..2952
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             2965..2991
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             5241..5306
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5340..5366
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             5412..5432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5477..5611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5501..5520)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5555..5574
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5728..5748)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5728..5746)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5767..5783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5767..5783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5780..5802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5791..5807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5815..5821,5822..5839,5840..5845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5860..5881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5998..6015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6169..6757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6249..6268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6928..7719,7720..7788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7551..7570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7789..7893)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7919..8374)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8056..8077)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8268..8287
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8448..9432
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atctgccacc atgggttcgg gccgtacgat gggagtgcag
      121 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
      181 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctctcg ggacagaaac
      241 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
      301 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
      361 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
      421 ctaaaactgg aaggcgccgg cggatgtttc gactctgttg aaatcagcgg agtggaggat
      481 cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac
      541 ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg
      601 tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac
      661 aaagtcatga aacagctcaa gagacgccga tatacaggat gggggcggct gtcaagaaaa
      721 ctgatcaatg gcatccgaga caagcagagt ggaaagacaa tcctggattt tcttaagtcc
      781 gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag
      841 gaggacatcc agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct
      901 aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat
      961 gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga
     1021 gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa
     1081 gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag
     1141 cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat
     1201 caggaactgg acatcaaccg gttgtccgac tacgacgtgg atcatatcgt gccccaaagc
     1261 tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg
     1321 aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag
     1381 ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga
     1441 ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc
     1501 cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa
     1561 aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat
     1621 ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat
     1681 gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagctggaa
     1741 tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct
     1801 gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt
     1861 ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca
     1921 aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccgcaag
     1981 gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc
     2041 tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat
     2101 tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt
     2161 gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc
     2221 atcacaatca tggagcgatc cagcttcgag aaaaacccca tcgactttct cgaagcgaaa
     2281 ggatataaag aggtcaaaaa agacctcatc attaagctgc ccaagtactc tctctttgag
     2341 cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag
     2401 ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc
     2461 aaagggtctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac
     2521 cttgatgaga tcatcgagca aataagcgag ttctccaaaa gagtgatcct cgccgacgct
     2581 aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag
     2641 gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag
     2701 tacttcgaca ccaccataga cagaaagaag tacacctcta caaaggaggt cctggacgcc
     2761 acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc
     2821 ggtggagacg gcaccggcgg gcccaagaag aagaggaagg tatacccata cgatgttcct
     2881 gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcgtatcc ttatgacgtt
     2941 ccagattacg ctggatccgc cgctccggca gctaagaaaa agaaactgga tttcgaatcc
     3001 ggagtaccta tggtggactt gaggacactc ggttattcgc aacagcaaca ggagaaaatc
     3061 aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg cgcttgtggg gcatggcttc
     3121 actcatgcgc atattgtcgc gctttcacag caccctgcgg cgcttgggac ggtggctgtc
     3181 aaataccaag atatgattgc ggccctgccc gaagccacgc acgaggcaat tgtaggggtc
     3241 ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc tgactgtggc gggtgagctt
     3301 agggggcctc cgctccagct cgacaccggg cagctgctga agatcgcgaa gagaggggga
     3361 gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc tcaccggggc ccccttgccc
     3421 ctgaacctga ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg
     3481 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     3541 caagtggtgg ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg
     3601 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     3661 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     3721 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag
     3781 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     3841 ccggaccaag tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg
     3901 cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca agtggtggct
     3961 atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     4021 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc
     4081 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     4141 ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa
     4201 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     4261 gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     4321 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     4381 aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     4441 catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg
     4501 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     4561 caagtggtgg ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg
     4621 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     4681 agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     4741 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag
     4801 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     4861 ccggaccaag tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg
     4921 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
     4981 atcgctaata ataacggagg acggccagcc ttggagtcca tcgtagccca attgtccagg
     5041 cccgatcccg cgttggctgc gttaacgaat gaccatctgg tggcgttggc atgtcttggt
     5101 ggacgacccg cgctcgatgc agtcaaaaag ggtctgcctc atgctcccgc attgatcaaa
     5161 agaaccaacc ggcggattcc cgagagaact tcccatcgag tcgcgggatc ttaatccgga
     5221 taagcggccg cctcgaggga gactacaaag accatgacgg tgattataaa gatcatgaca
     5281 tcgattacaa ggatgacgat gacaagcagg ggtccgagct cggatcgggt agagccgcgc
     5341 cggcagctaa gaaaaagaaa ctggatcaat tgggcgctag cggtagttct ggaggctcaa
     5401 gtggcgccgc tccaaagaag aagcgtaagg tatctgaatt ctgataatct agaactatag
     5461 tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     5521 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     5581 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     5641 tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc caatgcattg
     5701 ggcccggtac gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     5761 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5821 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5881 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5941 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6601 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     7921 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7981 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8041 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8101 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8161 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8221 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8281 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8341 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     8401 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagtcgac catagccaat
     8461 tcaatatggc gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat
     8521 tcaatatggc gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat
     8581 tcaatatggc ggacttggca ccatgccaat tcaatatggc ggacttggca ctgtgccaac
     8641 tggggagggg tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa
     8701 gtccgccata ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg
     8761 atcaatatat aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca
     8821 atactatgta ttggccctat gccatatagt attccatata tgggttttcc tattgacgta
     8881 gatagcccct cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca
     8941 tagccactcc cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata
     9001 tgggcggtcc tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc
     9061 ccgcctggct caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat
     9121 ggctcattgc ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg
     9181 gcccacttgg cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg
     9241 aaggacgcca gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc
     9301 gatggctgcc aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc
     9361 gttccattga cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct
     9421 cgtttaggga ac
//
