LOCUS       sequence-202459-        8760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Scot Wolfe
  TITLE     Inducible Cas9-DNA-binding-domain fusions for precise genome editing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             159..185
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             249..275
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             297..4397
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4410..4430
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4446..4448,4449..4769)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     polyA_signal    4811..4945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4835..4854)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4889..4908
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5058..5078)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5058..5076)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5097..5113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5097..5113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5110..5132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5121..5137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5145..5151,5152..5169,5170..5175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5190..5211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5328..5345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5499..6087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5579..5598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6258..7049,7050..7118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6881..6900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7119..7223)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7249..7704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7386..7407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7598..7617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(7778..8760,1..2)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcaa caagtttgta
      121 caaaaaagca ggctggcgcc accatgggta gagccgcgcc ggcagctaag aaaaagaaac
      181 tggatcaatt gggcgctagc ggtagtcccg ggtctggagg tagcggctca agtggccgta
      241 cggcagctcc tgcggcaaaa aagaaaaagt tggactctga attcggaagc ggaagcgaca
      301 agaagtactc cattgggctc gatatcggta ccaacagcgt cggctgggcc gtcattacgg
      361 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      421 taaagaagaa cctcattgga gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      481 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      541 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      601 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      661 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagctgg
      721 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg cacatgatca
      781 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      841 aactctttat ccaactggtt cagacttaca atcagctttt cgaggagaac ccgatcaacg
      901 catccggcgt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      961 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
     1021 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
     1081 agctgcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
     1141 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
     1201 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     1261 agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac
     1321 tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca
     1381 ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa
     1441 tggacggcac cgaggagctg ctggtaaagc tgaacagaga agatctgttg cgcaaacagc
     1501 gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc
     1561 tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa
     1621 tcctcacatt tcggataccc tactatgtag gccccctcgc tcggggaaat tccagattcg
     1681 cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag gaagtcgtgg
     1741 ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc
     1801 ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg
     1861 agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag
     1921 agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac
     1981 agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag
     2041 tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag
     2101 acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc
     2161 ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct
     2221 tcgacgacaa agtcatgaaa cagctcaaga gacgccgata tacaggatgg gggcggctgt
     2281 caagaaaact gatcaatggc atccgagaca agcagagtgg aaagacaatc ctggattttc
     2341 ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca
     2401 cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt cttcacgagc
     2461 acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg
     2521 tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga
     2581 tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga
     2641 ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa
     2701 acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt
     2761 acgtggatca ggaactggac atcaaccggt tgtccgacta cgacgtggat catatcgtgc
     2821 cccaaagctt tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaaa
     2881 atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt
     2941 ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg
     3001 ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg
     3061 agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt
     3121 acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg
     3181 tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc
     3241 atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca
     3301 agctggaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg
     3361 caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta
     3421 tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta
     3481 tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag
     3541 tccgcaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg
     3601 gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca
     3661 aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg
     3721 tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac
     3781 tgctgggcat cacaatcatg gagcgatcca gcttcgagaa aaaccccatc gactttctcg
     3841 aagcgaaagg atataaagag gtcaaaaaag acctcatcat taagctgccc aagtactctc
     3901 tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag
     3961 gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg
     4021 aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca
     4081 aacactacct tgatgagatc atcgagcaaa taagcgagtt ctccaaaaga gtgatcctcg
     4141 ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca
     4201 gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag
     4261 ccttcaagta cttcgacacc accatagaca gaaagaagta cacctctaca aaggaggtcc
     4321 tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct
     4381 ctcagctcgg tggagacggc accggcgggc ccaagaagaa gaggaaggta atcggatcct
     4441 ccggaatggg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc ttccccaagc
     4501 gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaatttgatt
     4561 cctctcggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag
     4621 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc
     4681 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg
     4741 tcttcgatgt ggagcttcta aaactggaac tcgagtaata atctagaact atagtgagtc
     4801 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4861 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4921 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     4981 tcagggggag gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg
     5041 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     5101 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5161 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5221 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5281 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5341 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5401 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5461 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     5941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     7261 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7321 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7381 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7441 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7501 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7561 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7621 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7681 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     7741 ggcgatcggt gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc
     7801 gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc
     7861 gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc
     7921 ggacttggca ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg
     7981 tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata
     8041 ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg atcaatatat
     8101 aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca atactatgta
     8161 ttggccctat gccatatagt attccatata tgggttttcc tattgacgta gatagcccct
     8221 cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc
     8281 cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc
     8341 tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct
     8401 caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc
     8461 ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg
     8521 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aaggacgcca
     8581 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
     8641 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
     8701 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
//
