LOCUS       sequence-202468-       13142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALE1 under the control of a 1.8kb elav enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
  TITLE     A multiplexable TALE-based binary expression system for in vivo 
            cellular interaction studies.
  JOURNAL   Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3.
  PUBMED    29162808
REFERENCE   2  (bases 1 to 13142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-21"; volume: "8(1):1663. doi"; pages: " 
            10.1038/s41467-017-01592-3"
COMMENT     Sequence Label: pJC-elav1.8kb-TALE1-VP64-P10
FEATURES             Location/Qualifiers
     source          1..13142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8547..8568
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          8600..8666
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             8704..8724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8725..8751
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             12244..12393
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(13088..13106)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgagtc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggaaac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgagtca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttattcccc acttaagcat cgcatttgca tatttgtatg ctaaccaaac
     6541 tctctgcgac gtcgatctgt ctgaatctga atccgcatat ctttcgaggg cagcaatagc
     6601 gccaccccac cccatttccg ttgccactag cagcgcacca ccctttgaaa ggacatcaaa
     6661 aaccaccccc tgcccaaagt tgcgttacaa ttcccgaagt gcagtgcagc gtacataaaa
     6721 catacaatga aatccgataa aattttctat gtcctttata tcctcataac atgcccaact
     6781 ataaaccgag ccaaattctt ctcgtgaaaa agttgcacac aacgcgattt cactgtacct
     6841 tacattgttc tgtaaactgg aacagagcgt ccgactggaa gaggcgaagg aaaaaatcag
     6901 cacagggaac aggaagttca caaggattgg gggtgtccga gggtggctac atatatatgg
     6961 cgatatatgg catacagtat gtgcatacat atacatacat atacacatat atgtatgtat
     7021 gtaccatact atgtggcgtg tctagtggcg cgaataacga atacaggagc aatgaagcac
     7081 acgaacgaag cgaaacgaaa cgaagggaaa tattgaaaaa gccagaattg cattacttca
     7141 aaactatacc ggctcttgga tatgggtcct gggtctcggt tccttgcttc tggctttcgc
     7201 ttcgtagggg gtgtacgagc aatactgcca ggagaaacca ccctagcctt tggcgttggt
     7261 ggttgatagt gcaaaaataa taataacgga aacggaacca taaacccaaa acagccctta
     7321 caacgcgact gatttgtaag gataatggtc gcatgcatca taaaacggtt gacgacctcg
     7381 tcggaaagag ccaacatttt gcaatgattt ccaattgctc ctgctatcta ggcccaatgg
     7441 gccgctaagt acatatgaag catacatagc taaatacata tgaagcatac atacatcggg
     7501 ttaactataa aaccaatgtg ttctagatga gtttgttaat tttacaaagt aagttagggc
     7561 tgctccagtg gttttaagtt gcttaactgg acaagagaac ttggctctct cccccatcta
     7621 tctcttgtct tcgggaaagg taacctccat tttgatcggt tgaatttgta tcaaaaagca
     7681 tgaaaaactt tgaatacagc ataaaccact gagagagcag ccgaaagcca ggggtgtatc
     7741 aaaaggggtt cttagaaata ttccactttg caacgattat agactcgttt cttatcagac
     7801 aaaactttct ccacttctac gtggtattta cagaaacaaa acttaaggta aaatgttagg
     7861 tggctatata ttaaagctat taaaatatat taaaggtttc cggaataacc ccctatctcg
     7921 gcgcgcagat acgcccatgt cgccactctt tcgctctcta ccttccactc tcgctccgaa
     7981 caccattgca accccgagag agctgctcga gaggcaacta tgagatatga gaatgagagg
     8041 ttgctctatg tgaaaatgga aaccgaaacc gtcaatgcct caatttcgca tttcattatt
     8101 tcatttccac gccaaccatc gtgcgccgcg gtctgaacgc tcctgccaca gaaaaagaaa
     8161 ataaaagcaa ggaaaattct aatcgaatac caaatatcgt gcttgtgtgc tctttccgca
     8221 attgattttt tttaagtagt gcatgacaat aaccgttgag ttgactccaa ccgaagtaac
     8281 cataactgga agctgacaac agccattggc cggaatctca tctcacaccg agcgaaatgt
     8341 aagacatagg ccggccatac gccggagtat aaatagaggc gcttcgtcta cggagcgaca
     8401 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
     8461 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
     8521 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
     8581 aatacaagag atctaaaagg taggttcaac cactgatgcc taggcacacc gaaacgacta
     8641 accctaattc ttatccttta cttcaggcgg ccgcggctcg agctcgagat ggcttcctcc
     8701 cctccaaaga aaaagagaaa ggttagttgg aaggacgcaa gtggttggtc tagaatgcat
     8761 gcggatccca ttcgtccgcg caggccaagt cctgcccgcg agcttctgcc cggaccccaa
     8821 ccggataggg ttcagccgac tgcagatcgt ggggtgtctg cgcctgctgg cagccctctg
     8881 gatggcttgc ccgctcggcg gacggtgtcc cggacccggc tgccatctcc ccctgcgccc
     8941 tcacctgcgt tctcggcggg cagcttcagc gatctgctcc gtccgttcga tccgtcgctt
     9001 cttgatacat cgcttcttga ttcgatgcct gccgtcggca cgccgcatac agcggctgcc
     9061 ccagcagagt gggatgaggc gcaatcggct ctgcgtgcag ccgatgaccc gccacccacc
     9121 gtgcgtgtcg ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc gcgacggcgt
     9181 gctgcgcaac cctccgacgc ttcgccggcc gcgcaggtgg atctacgcac gctcggctac
     9241 agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac
     9301 gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg
     9361 gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg
     9421 acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc
     9481 ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt
     9541 gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat
     9601 gcactgacgg gtgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac
     9661 attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     9721 catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg
     9781 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     9841 caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg
     9901 ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc
     9961 agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
    10021 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag
    10081 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
    10141 ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg
    10201 cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca agtggtggct
    10261 atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
    10321 ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc
    10381 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
    10441 ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca agcgctcgaa
    10501 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg
    10561 gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg
    10621 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
    10681 aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
    10741 catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg
    10801 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
    10861 caagtggtgg ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg
    10921 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
    10981 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
    11041 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag
    11101 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
    11161 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
    11221 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
    11281 atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
    11341 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc
    11401 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
    11461 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
    11521 agcattgtgg cccagctgag ccggcctgat ccggcgttgg ccgcgttgac caacgaccac
    11581 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
    11641 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
    11701 cgcgttgccg actacgcgca agtggttcgc gtgctggagt ttttccagtg ccactcccac
    11761 ccagcgtacg catttgatga ggccatgacg cagttcggga tgagcaggaa cgggttggta
    11821 cagctctttc gcagagtggg cgtcaccgaa ctcgaagccc gcggtggaac gctcccccca
    11881 gcctcgcagc gttgggaccg tatcctccag gcatcaggga tgaaaagggc caaaccgtcc
    11941 cctacttcag ctcaaacacc ggatcaggcg tctttgcatg cattcgccga ttcgctggag
    12001 cgtgaccttg atgcgcccag cccaatgcac gagggagatc agacgcgggc aagcagccgt
    12061 aaacggtccc gatcggatcg tgctgtcacc ggcccctccg cacagcaggc tgtcgaggtg
    12121 cgcgttcccg aacagcgcga tgcgctgcat ttgcccctca gctggagggt aaaacgcccg
    12181 cgtaccagga tctggggcgg cctcccggat ccgatatcta gatcccaggg ttccggacgg
    12241 gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt
    12301 gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt
    12361 gacgcccttg atgatttcga cctggacatg ctgatctaga aatgaatcgt ttttaaaata
    12421 acaaatcaat tgttttataa tattcgtacg attctttgat tatgtaataa aatgtgatca
    12481 ttaggaagat tacgaaaaat ataaaaaata tgagttctgt gtgtataaca aatgctgtaa
    12541 acgccacaat tgtgtttgtt gcaaataaac ccatgattat ttgattaaaa ttgttgtttt
    12601 ctttgttcat agacaatagt gtgttttgcc taaacgtgta ctgcataaac tccatgcgag
    12661 tgtatagcga gctagtggct aacgcttgcc ccaccaaagt agattcgtca aaatcctcaa
    12721 tttcatcacc ctcctccaag tttaacattt ggccgtcgga attaacttct aaagatgcca
    12781 cataatctaa taaatgaaat agagattcaa acgtggcgtc atcgtccgtt tcgaccattt
    12841 ccgaaaagaa ctcgggcata aactctatga tttctctgga cgtggtgttg tcgaaactct
    12901 caaagtacgc agtcaggaac gtgcgcgaca tgtcgtcggg aaactcgcgc ggaaacatgt
    12961 tgttgtaacc gaacgggtcc catagcgcca aaaccaaatc tgccagcgtc aatagaatga
    13021 gcacgatgcc gacaatggag ctggcttgga tagcgattcg agttaacgaa ttcttgaaga
    13081 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
    13141 ta
//
