LOCUS       sequence-202471-       13689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALE3 under the control of a 2.4kb mhc enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
  TITLE     A multiplexable TALE-based binary expression system for in vivo 
            cellular interaction studies.
  JOURNAL   Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3.
  PUBMED    29162808
REFERENCE   2  (bases 1 to 13689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-21"; volume: "8(1):1663. doi"; pages: " 
            10.1038/s41467-017-01592-3"
COMMENT     Sequence Label: pJC-mhc2.4kb-TALE3-VP64-P10
FEATURES             Location/Qualifiers
     source          1..13689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9094..9115
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          9147..9213
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             9251..9271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9272..9298
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             12791..12940
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(13635..13653)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgagtc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggaaac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgagtca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttctagaaa tgatatgtga aataagtaaa aagaaagctc cccttggaaa
     6541 gagcgaggga ttccggcgga gaagaattgc ctcacggcgg aaaagttgtc attgaaatga
     6601 attttactat ttgactaatg ggagaattgc gagagagcct acgcaatggc aatcgtcgga
     6661 gaaggagctt tccgctctcg attccgcaac gatcctgatt gcatcatatg ctcacacata
     6721 tatcatatcc accatcccgc aatcccccat agaaatgccc acttgctaga gaagaggaat
     6781 aggaatcaga gcagccaagt gctagaccga aaaagggagc aagaaaaacc gaaacagaaa
     6841 cagcgccacg cacaccgatc gtcgaatcga aaagctttcg gggtcttacg ggatccatgg
     6901 gtatcaagtt gccccgtata aaaggcaagt ttaccggttg cacggttcag aacggctttc
     6961 gagcgcgtaa gtgcaacacc tttgatgtgc gatctctgat ttttggatat taccacccag
     7021 cagcagtagc ggtagcagca gcatcagcgg cagcagcaac aatttgacct tttggtcaaa
     7081 aagcataaaa ctaaaataat tattactacc tatatatatt tatatatgca acaaaatcga
     7141 gaaatttaaa aaagtgtgtg tgtggtgctt taactacgat ccgagcgaaa acggatctat
     7201 taaaactctg tgacaatcag aacagtgaac cggaatacct cggattatcg tatcgaattg
     7261 gattacaaat aacaatcttt tcttaggaag tacatcagtt acttaacata acctataggc
     7321 gttaatttgt tttctgagaa gtatgcaact atcttcaact tcttgtgtaa caaatctttg
     7381 actttcgatc atatcagcga aaaaatctga aagtgaagta agaaggatat aaggaaactt
     7441 ttaggattct cacgaaaaac tattttatcc tttctcgccc aatcattttg gattaaaagt
     7501 cttcaatcgt cctatttttc ggctacttaa aaaattctaa agcaaataga tcatttaaat
     7561 gtcagaaaga gacagccagc tgttacttca tcaaaagaaa gattcgtgtt tcgatgtgag
     7621 ttcgaaatgc tgtaatagcc atttcgaatt actggcgaaa tgatttcata cacaaatacc
     7681 tgtgtggccg agacatatgc gtatgcatac tatagaaaat agatttagaa tactcgaatt
     7741 cgttgtcggc tcatatacat gggcgaaata atttcgaata tgttttaaaa ataaccaaag
     7801 acattagaaa gagatcgcca atacttatac attatgtcta tgtgtgccat gtggtagcat
     7861 gagccaaaaa gcttctcgaa attacgaatt acatatagac gaatatgtat gtgactttag
     7921 tttcgaaata atttcgagaa ttttaaaaat aacgcattcg ttaaaagttc gcgtcaattc
     7981 gaatcgaatt ttcgattgtc gatttagtgt ggattgtcga aaatcgttcc gccttcgaag
     8041 tttactgaaa ggaatcattg cgatctcgta aattgcttgt atgagaacac gccaccatat
     8101 cgagatacct gtttaaaata tcgaaatcgc gttccaaacg gtagtagtta ccagttgagt
     8161 gagttgtggt gacagttgtt taacctctct acatttgtaa taagcattgt tttggcgtat
     8221 caatagtctg tagatttttt cacaaagaaa cctggagaaa agtacacaaa tatctaaatg
     8281 aaaattggcg ttacgtaaca aataccaacg ccccgaaaat ccgtatatat gcaaagcatt
     8341 ctgatatata tatatataag tggtatacat atacatattc gtatcggatc cgtgatctat
     8401 atttaggcga caacccacac aattcacttg ttgtctgttt ataacaaaaa caacaacaca
     8461 agcaccgacc aggcctaatt aactttgagg tgtgagataa aatcaaagcc acacataaat
     8521 aaatatccgg ttacggtaac gggaatttcg aaagcgcgaa acgggaaaaa tgttcatatt
     8581 tggaaatcga aattaaattt cgaaatccta tccacgcaca cacgcgtaga actgttatag
     8641 tgtccgctat tgctgctgct gtcaaaatgg gtcaaaagca taaaatccat aaaagaatca
     8701 gacttcgaaa caaaattcca aaaatagtat gcttttctga gaaccatata ataaatatta
     8761 tttttctttt gtatatttac agggaagttt tgggctcacg acgcgattaa caaagaaatt
     8821 ttgaaaagtc ccaatattat ttttcgagtt tttaagtttc taaacgaaat ttaagcaagg
     8881 ataatgccat agctagcgag acgatacgcc ggagtataaa tagaggcgct tcgtctacgg
     8941 agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat
     9001 aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca
     9061 attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta
     9121 attattgaat acaagagatc taaaaggtag gttcaaccac tgatgcctag gcacaccgaa
     9181 acgactaacc ctaattctta tcctttactt caggcggccg cggctcgagc tcgagatggc
     9241 ttcctcccct ccaaagaaaa agagaaaggt tagttggaag gacgcaagtg gttggtctag
     9301 aatgcatgcg gatcccattc gtccgcgcag gccaagtcct gcccgcgagc ttctgcccgg
     9361 accccaaccg gatagggttc agccgactgc agatcgtggg gtgtctgcgc ctgctggcag
     9421 ccctctggat ggcttgcccg ctcggcggac ggtgtcccgg acccggctgc catctccccc
     9481 tgcgccctca cctgcgttct cggcgggcag cttcagcgat ctgctccgtc cgttcgatcc
     9541 gtcgcttctt gatacatcgc ttcttgattc gatgcctgcc gtcggcacgc cgcatacagc
     9601 ggctgcccca gcagagtggg atgaggcgca atcggctctg cgtgcagccg atgacccgcc
     9661 acccaccgtg cgtgtcgctg tcactgccgc gcggccgccg cgcgccaagc cggccccgcg
     9721 acggcgtgct gcgcaaccct ccgacgcttc gccggccgcg caggtggatc tacgcacgct
     9781 cggctacagt cagcagcagc aagagaagat caaaccgaag gtgcgttcga cagtggcgca
     9841 gcaccacgag gcactggtgg gccatgggtt tacacacgcg cacatcgttg cgctcagcca
     9901 acacccggca gcgttaggga ccgtcgctgt cacgtatcag cacataatca cggcgttgcc
     9961 agaggcgaca cacgaagaca tcgttggcgt cggcaaacag tggtccggcg cacgcgccct
    10021 ggaggccttg ctcacggatg cgggggagtt gagaggtccg ccgttacagt tggacacagg
    10081 ccaacttgtg aagattgcaa aacgtggcgg cgtgaccgca atggaggcag tgcatgcatc
    10141 gcgcaatgca ctgacgggtg cccccctgaa cctgaccccg gaccaagtgg tggctatcgc
    10201 cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
    10261 ccaggaccat ggcctgactc cggaccaagt ggtggctatc gccagccacg atggcggcaa
    10321 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
    10381 tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
    10441 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
    10501 tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
    10561 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
    10621 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
    10681 cctgaccccg gaccaagtgg tggctatcgc cagcaacaat ggcggcaagc aagcgctcga
    10741 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc cggaccaagt
    10801 ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
    10861 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
    10921 cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
    10981 ccatggcctg accccggacc aagtggtggc tatcgccagc aacaatggcg gcaagcaagc
    11041 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
    11101 ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg
    11161 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
    11221 cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
    11281 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca atggcggcaa
    11341 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
    11401 tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
    11461 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
    11521 tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
    11581 gctgtgccag gaccatggcc tgactccgga ccaagtggtg gctatcgcca gccacgatgg
    11641 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
    11701 cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga
    11761 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
    11821 ggtggctatc gccagcaaca atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
    11881 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
    11941 cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
    12001 ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc
    12061 gctcgaaagc attgtggccc agctgagccg gcctgatccg gcgttggccg cgttgaccaa
    12121 cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa
    12181 gggattgccg cacgcgccgg aattgatcag aagagtcaat cgccgtattg gcgaacgcac
    12241 gtcccatcgc gttgccgact acgcgcaagt ggttcgcgtg ctggagtttt tccagtgcca
    12301 ctcccaccca gcgtacgcat ttgatgaggc catgacgcag ttcgggatga gcaggaacgg
    12361 gttggtacag ctctttcgca gagtgggcgt caccgaactc gaagcccgcg gtggaacgct
    12421 ccccccagcc tcgcagcgtt gggaccgtat cctccaggca tcagggatga aaagggccaa
    12481 accgtcccct acttcagctc aaacaccgga tcaggcgtct ttgcatgcat tcgccgattc
    12541 gctggagcgt gaccttgatg cgcccagccc aatgcacgag ggagatcaga cgcgggcaag
    12601 cagccgtaaa cggtcccgat cggatcgtgc tgtcaccggc ccctccgcac agcaggctgt
    12661 cgaggtgcgc gttcccgaac agcgcgatgc gctgcatttg cccctcagct ggagggtaaa
    12721 acgcccgcgt accaggatct ggggcggcct cccggatccg atatctagat cccagggttc
    12781 cggacgggct gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga
    12841 tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct
    12901 cggcagtgac gcccttgatg atttcgacct ggacatgctg atctagaaat gaatcgtttt
    12961 taaaataaca aatcaattgt tttataatat tcgtacgatt ctttgattat gtaataaaat
    13021 gtgatcatta ggaagattac gaaaaatata aaaaatatga gttctgtgtg tataacaaat
    13081 gctgtaaacg ccacaattgt gtttgttgca aataaaccca tgattatttg attaaaattg
    13141 ttgttttctt tgttcataga caatagtgtg ttttgcctaa acgtgtactg cataaactcc
    13201 atgcgagtgt atagcgagct agtggctaac gcttgcccca ccaaagtaga ttcgtcaaaa
    13261 tcctcaattt catcaccctc ctccaagttt aacatttggc cgtcggaatt aacttctaaa
    13321 gatgccacat aatctaataa atgaaataga gattcaaacg tggcgtcatc gtccgtttcg
    13381 accatttccg aaaagaactc gggcataaac tctatgattt ctctggacgt ggtgttgtcg
    13441 aaactctcaa agtacgcagt caggaacgtg cgcgacatgt cgtcgggaaa ctcgcgcgga
    13501 aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa ccaaatctgc cagcgtcaat
    13561 agaatgagca cgatgccgac aatggagctg gcttggatag cgattcgagt taacgaattc
    13621 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    13681 ggtttctta
//
