LOCUS       sequence-202476-       13239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALE4 under the control of a 1.9kb repo enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
  TITLE     A multiplexable TALE-based binary expression system for in vivo 
            cellular interaction studies.
  JOURNAL   Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3.
  PUBMED    29162808
REFERENCE   2  (bases 1 to 13239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-21"; volume: "8(1):1663. doi"; pages: " 
            10.1038/s41467-017-01592-3"
COMMENT     Sequence Label: pJC-repo1.9kb-TALE4-VP64-P10
FEATURES             Location/Qualifiers
     source          1..13239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8644..8665
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          8697..8763
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             8801..8821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8822..8848
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             12341..12490
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(13185..13203)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgagtc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggaaac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgagtca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttttcctgg gaagctgaag tttctttgct gtttttcatt cgatcaatgc
     6541 gggccggtct ttgaggacag aactttgcca ttggattgtg caactttgtc ttaatttaga
     6601 caccaaaaca ctttacttcc ttaacatctt ttaaaaaagg ttatggtttt tgtttgcttt
     6661 agtcgaattg gtattcaatt aacatcaggt attgatcttt tatttagtaa aagaataaat
     6721 aatgaagcta tcaattcttc aacacccaat atatttccaa atattatcac caactttgca
     6781 cttcaaattg gattgatgat gattggattt ggccatcgaa tttgttagat gtattttcat
     6841 attgatttta tagtatttaa taaatatcta aataagttac tcttaaagat caccgatccc
     6901 aatgtccatg tgaatgtata gctctaccca ctttcaccct cttcgattgc aatgcccaca
     6961 tgattcagca gccttcttcc cacttcccat tcaaatgctc cgtgaaatct ttggatctgt
     7021 ccaaatgagc cataacttag cccctgacta agtgcccacc tcgacgcgat cgaagtacca
     7081 gcatctggcc aaaaggtcac acgcaacagc ggactggagg aaaagggagc atgccaatcc
     7141 ttgagagggt gacacgcgct gtgggaagat cgagcgatca gggtgtggaa acgggctgaa
     7201 gtggaagaag aaggtcacaa gcgtgccacc aagtcgctgc cttacagggg ttgcaacctc
     7261 gaatggaacc accctcatga gtccaattgg aaccacgacc atcggaattc ctcggctaga
     7321 agttaattgt tcgtccaaca tgtgtgacga tgaaggtgca ttgttacgaa tagcgatgac
     7381 catgaggtgg acgcgaaata gatgggctct ctcaactttg ggcgaaacta atctgtatca
     7441 atggattgcc aacaagaagc cgctacataa gtgcatatgg aaatatagtg gatgttaaaa
     7501 accccccttt ttaggtttta tctgtgatac gatctttaga acgattagaa agcagcatag
     7561 taattttcat ctctccgcca acattgaatg ctttaatatg gctataacta ttctcactag
     7621 tttgataaac ggttctgaaa tcgaaaacat ttgaaaagat agtatagtat atataccaga
     7681 cataaactta atccttcaaa tcgttcaaca ttcttcttat ttcgatcccc aatcttatag
     7741 ataccttatc tccatatatc gaatcataga tcataccata gattatcttc tgtacaatta
     7801 ttattaatag gtatgcgata gtttgtaact gcatggtaac agaacacaat cagttcattt
     7861 atatgatatc tcataatatg actatgcttt catggttcta tatcctcatt ataaatgaaa
     7921 tctaaataaa acataattca atagtaggat aacaataaga tcagcgtgcc attagagtcc
     7981 ttaaatccgc tctaagaact gatagccagt agagatgcaa gtcccgttcc caggacccca
     8041 tcatcctgtc gtgctcttcc gttcccagaa catctcgatc tgctggtagc cgtgtgcccg
     8101 cgaagctgaa gaccaaaaac tggacccagg cagctagacc cagtcaaaaa taaaaaaaca
     8161 gaaaaaagaa gaaaaaatcg caaagtaggg gagaccacac cccgctgaat ggaaaatcgt
     8221 cgaatcgctg ggcaagcgga aaagttacac gcgccagcat gaaacgcaca gagcggcgtt
     8281 cgcatgcgcc gtcccgctcg cacggcgata gtgtcggccg atggagcgat gcgattggtc
     8341 gctgccagcg ctgctgctgc gctgccgcct gcgccggtat aaaaggacaa tcatcgccag
     8401 tagccgagtc aattaaggcc aaagactcga gcggtgacat agctagcgag acgatacgcc
     8461 ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg
     8521 aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat
     8581 ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca
     8641 actgcaacta ctgaaatctg ccaagaagta attattgaat acaagagatc taaaaggtag
     8701 gttcaaccac tgatgcctag gcacaccgaa acgactaacc ctaattctta tcctttactt
     8761 caggcggccg cggctcgagc tcgagatggc ttcctcccct ccaaagaaaa agagaaaggt
     8821 tagttggaag gacgcaagtg gttggtctag aatgcatgcg gatcccattc gtccgcgcag
     8881 gccaagtcct gcccgcgagc ttctgcccgg accccaaccg gatagggttc agccgactgc
     8941 agatcgtggg gtgtctgcgc ctgctggcag ccctctggat ggcttgcccg ctcggcggac
     9001 ggtgtcccgg acccggctgc catctccccc tgcgccctca cctgcgttct cggcgggcag
     9061 cttcagcgat ctgctccgtc cgttcgatcc gtcgcttctt gatacatcgc ttcttgattc
     9121 gatgcctgcc gtcggcacgc cgcatacagc ggctgcccca gcagagtggg atgaggcgca
     9181 atcggctctg cgtgcagccg atgacccgcc acccaccgtg cgtgtcgctg tcactgccgc
     9241 gcggccgccg cgcgccaagc cggccccgcg acggcgtgct gcgcaaccct ccgacgcttc
     9301 gccggccgcg caggtggatc tacgcacgct cggctacagt cagcagcagc aagagaagat
     9361 caaaccgaag gtgcgttcga cagtggcgca gcaccacgag gcactggtgg gccatgggtt
     9421 tacacacgcg cacatcgttg cgctcagcca acacccggca gcgttaggga ccgtcgctgt
     9481 cacgtatcag cacataatca cggcgttgcc agaggcgaca cacgaagaca tcgttggcgt
     9541 cggcaaacag tggtccggcg cacgcgccct ggaggccttg ctcacggatg cgggggagtt
     9601 gagaggtccg ccgttacagt tggacacagg ccaacttgtg aagattgcaa aacgtggcgg
     9661 cgtgaccgca atggaggcag tgcatgcatc gcgcaatgca ctgacgggtg cccccctgaa
     9721 cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga
     9781 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
     9841 ggtggctatc gccagcaaca atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     9901 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     9961 cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
    10021 ccatggcctg accccggacc aagtggtggc tatcgccagc aacaatggcg gcaagcaagc
    10081 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
    10141 ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg
    10201 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
    10261 cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
    10321 ccaggaccat ggcctgactc cggaccaagt ggtggctatc gccagccacg atggcggcaa
    10381 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
    10441 tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
    10501 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc aagtggtggc
    10561 tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
    10621 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg
    10681 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
    10741 cctgaccccg gaccaagtgg tggctatcgc cagcaacaat ggcggcaagc aagcgctcga
    10801 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
    10861 ggtggctatc gccagcaaca atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
    10921 gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca
    10981 cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
    11041 ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc
    11101 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
    11161 ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg
    11221 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
    11281 cagcaacaat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
    11341 ccaggaccat ggcctgactc cggaccaagt ggtggctatc gccagccacg atggcggcaa
    11401 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
    11461 tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
    11521 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
    11581 tatcgccagc aacggtggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg
    11641 gcctgatccg gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg
    11701 cggacgtcct gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag
    11761 aagagtcaat cgccgtattg gcgaacgcac gtcccatcgc gttgccgact acgcgcaagt
    11821 ggttcgcgtg ctggagtttt tccagtgcca ctcccaccca gcgtacgcat ttgatgaggc
    11881 catgacgcag ttcgggatga gcaggaacgg gttggtacag ctctttcgca gagtgggcgt
    11941 caccgaactc gaagcccgcg gtggaacgct ccccccagcc tcgcagcgtt gggaccgtat
    12001 cctccaggca tcagggatga aaagggccaa accgtcccct acttcagctc aaacaccgga
    12061 tcaggcgtct ttgcatgcat tcgccgattc gctggagcgt gaccttgatg cgcccagccc
    12121 aatgcacgag ggagatcaga cgcgggcaag cagccgtaaa cggtcccgat cggatcgtgc
    12181 tgtcaccggc ccctccgcac agcaggctgt cgaggtgcgc gttcccgaac agcgcgatgc
    12241 gctgcatttg cccctcagct ggagggtaaa acgcccgcgt accaggatct ggggcggcct
    12301 cccggatccg atatctagat cccagggttc cggacgggct gacgcattgg acgattttga
    12361 tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga
    12421 tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct
    12481 ggacatgctg atctagaaat gaatcgtttt taaaataaca aatcaattgt tttataatat
    12541 tcgtacgatt ctttgattat gtaataaaat gtgatcatta ggaagattac gaaaaatata
    12601 aaaaatatga gttctgtgtg tataacaaat gctgtaaacg ccacaattgt gtttgttgca
    12661 aataaaccca tgattatttg attaaaattg ttgttttctt tgttcataga caatagtgtg
    12721 ttttgcctaa acgtgtactg cataaactcc atgcgagtgt atagcgagct agtggctaac
    12781 gcttgcccca ccaaagtaga ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt
    12841 aacatttggc cgtcggaatt aacttctaaa gatgccacat aatctaataa atgaaataga
    12901 gattcaaacg tggcgtcatc gtccgtttcg accatttccg aaaagaactc gggcataaac
    12961 tctatgattt ctctggacgt ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg
    13021 cgcgacatgt cgtcgggaaa ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat
    13081 agcgccaaaa ccaaatctgc cagcgtcaat agaatgagca cgatgccgac aatggagctg
    13141 gcttggatag cgattcgagt taacgaattc ttgaagacga aagggcctcg tgatacgcct
    13201 atttttatag gttaatgtca tgataataat ggtttctta
//
